查询词典 blotting

The protein and mRNA expressions of Bcl-2 and Bax were detected by Western blotting and RT-PCR, respectively.

Western blotting和RT-PCR检测各组细胞不同时点Bcl-2和Bax蛋白、mRNA的表达。

Moreover, the protein expressions of all kinds of integrin subunits were also detected with Western blotting.

同时用Western blotting检测各种整合素亚单位的蛋白表达变化。

The expressing of P65 was analyzed by RT-PCR and Western blotting.

RT-PCR和Western blotting检测基因转染对P65表达的影响。

The mRNA and protein expression of Cyclin D1 and p27 were detected by RT-PCR and Western Blotting respectively.

RT-PCR 和Western Blotting法分别检测细胞周期调节蛋白D1(Cyclin D1)和P27 mRNA和蛋白水平的表达变化。

The mRNA and protein of CD14 and TLR4 in U937 cells were detected by semi-quantitative RT-PCR and Western blotting.

RT-PCR 和Western blotting检测U937 细胞膜受体CD_(14)和跨膜受体钟

Ion-exchange chromatography with DE-52 was used for the purification of the antigens. The purified products were characterized by Western-blotting. Result For the pollen extract of Caryota ochlandra, 30 protein bands were detected in SDS-PAGE.

收集过敏病人血清，采用免疫印迹(Western-blotting)法鉴定其变应原成分，通过离子交换层析对花粉变应原进行初步纯化和免疫印迹鉴定。

Select chorioallantoic membrane vascular extract protein of different time points , Detect FGF2 and TGF-β2 protein in chorioallantoic membrane of this three-stage by Western blotting, judge whether to establish a model of dynamic vascular development successful.

选取不同时间点的尿囊膜血管，提取蛋白，用Western blotting技术检测FGF2和TGF-β2蛋白在血管发育的这三个阶段的表达量的变化，判断动态血管发育模型是否建立成功。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

Methods MTT assay for detection of single use of Adriamycin and combination of Adriamycin with gleditsia saponin on the Role of the SMMC-7721 cell survival rate; detection of mRNA expression of bcl-2 by PCR method; detection of protein expression of bcl-2 by Western blotting method.

MTT法检测单独应用阿霉素或阿霉素与皂角皂苷联合作用的SMMC-7721细胞的生存率；RT-PCR检测bcl-2 mRNA表达；Western blotting检测bcl-2蛋白的表达。

The specificity of polyclonal antibody was detected with Western blotting, which applied rabbit antiserum and sera of known Humulus pollen allergic patients.

同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。