查询词典 blotting

ATP content was detected by high performance liquid chromatogram. Mitochondrial membrane potential was measured by rhodamine123 (Rh123) staining and photofluorography. The activity of superoxide dismutase was observed using a SOD kit. The expression of heme oxygenase-1 (HO-1) was evaluated by Western blotting.

应用高效液相色谱法检测细胞内ATP的含量；罗丹明123(Rh123)染色荧光显微镜照相检测线粒体膜电位；超氧化物歧化酶检测试剂盒检测SOD活性；免疫印迹法检测血红素氧合酶－1(HO-1)的表达。

It can be used as a versatile instrument for techniques like gel staining, western blotting, in site hybridisation on fixed tissue as well as for approaches on embyos(mouse, frog, zebrafish and many others).

它作为凝胶的技术用途广泛的器具使用，西方在固定的薄纱织品上的位置杂交和为在胚胎上的方法(老鼠、青蛙、 zebrafish 和许多其他)。

The protein expressions of MRP3 and Lrh-1 were determined with Western blotting. Results Total bilirubin and direct bilirubin increased, but albumin decreased. In BDL rat liver, the membrane protein MRP3 and Lrh-1 expression were all significantly up-regulated.

结果 梗阻性黄疸大鼠总胆红素、直接胆红素显著升高，白蛋白显著降低；与假手术对照组相比，梗阻性黄疸组大鼠肝细胞MRP3和Lrh-1蛋白表达明显升高(P值分别为0.027和0.046)，差异有统计学意义。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

Golden Delicious using a combination of carboxyfluorescein transport, immunogold electron microscopy, and immunoblotting assay.

利用 Western Blotting 和胶体金免疫电镜定位技术，对苹果果实中参与卸载的己糖转运蛋白和酸性转化酶在韧皮部及其周围薄壁组织中的定位进行了研究，并初步研究了脱落酸对己糖转运蛋白表达量的影响。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。