查询词典 blotting

ATP content was detected by high performance liquid chromatogram. Mitochondrial membrane potential was measured by rhodamine123 (Rh123) staining and photofluorography. The activity of superoxide dismutase was observed using a SOD kit. The expression of heme oxygenase-1 (HO-1) was evaluated by Western blotting.

应用高效液相色谱法检测细胞内ATP的含量；罗丹明123(Rh123)染色荧光显微镜照相检测线粒体膜电位；超氧化物歧化酶检测试剂盒检测SOD活性；免疫印迹法检测血红素氧合酶－1(HO-1)的表达。

It can be used as a versatile instrument for techniques like gel staining, western blotting, in site hybridisation on fixed tissue as well as for approaches on embyos(mouse, frog, zebrafish and many others).

它作为凝胶的技术用途广泛的器具使用，西方在固定的薄纱织品上的位置杂交和为在胚胎上的方法(老鼠、青蛙、 zebrafish 和许多其他)。

The protein expressions of MRP3 and Lrh-1 were determined with Western blotting. Results Total bilirubin and direct bilirubin increased, but albumin decreased. In BDL rat liver, the membrane protein MRP3 and Lrh-1 expression were all significantly up-regulated.

结果 梗阻性黄疸大鼠总胆红素、直接胆红素显著升高，白蛋白显著降低；与假手术对照组相比，梗阻性黄疸组大鼠肝细胞MRP3和Lrh-1蛋白表达明显升高(P值分别为0.027和0.046)，差异有统计学意义。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

Golden Delicious using a combination of carboxyfluorescein transport, immunogold electron microscopy, and immunoblotting assay.

利用 Western Blotting 和胶体金免疫电镜定位技术，对苹果果实中参与卸载的己糖转运蛋白和酸性转化酶在韧皮部及其周围薄壁组织中的定位进行了研究，并初步研究了脱落酸对己糖转运蛋白表达量的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐