查询词典 blotting

RT-PCR was a sensitive and stable method. It would be useful for large-scale diagnosis.A length cDNA of CEVd had been cloned into plasmid vector. Biotinylated probes were prepared from these plasmid DNAs. CEVd were examined from the positive and netative materials by Dot-blotting hybridization and imprint-hybridization. The result was exposed to x-ray film. Singles were detected for positive materials spots probed with probe, indicating that under the reaction conditions the probe hybridized with the target viroid specifically.

核酸杂交法检测：利用已经克隆的美国柑桔裂皮类病毒cDNA序列，制备生物素标记的核苷酸探针，采用斑点杂交的方法和组织印迹的方法分别对阳性材料进行杂交检测，通过胶片曝光显示杂交信号，发现在两种方法的检测结果中阳性材料都有强的反应，而阴性材料无反应或较弱，该方法安全可靠、快速准确，适合大量样品的检测。

Methods After feeding a high-cholesterol diet to guinea pigs, Oddi′s sphincters were disseced on day 30 and day 60 respectively. We used RT-PCR, western-blotting to evaluate expression level of calponin gene.

饲予豚鼠高胆固醇饮食，分别在30天和60天取出Oddi括约肌，应用逆转录聚合酶链反应和蛋白质免疫印迹法检测动物Oddi括约肌调宁蛋白基因的表达水平。

Behavioral changes in rats after stress were observed by open-field test,and neurogranin level of hippocampus and forebrain were determined by Western blotting.

以旷场行为任务来评定大鼠应激后的行为变化，W estern b lotting方法测定海马和前脑皮层中的NG含量。

The expressions of thedifferentially expressed genes (gamma-A-crystallin and neural tropomodulin) werefurther analyzed in the retinae of rds, rd and C3B mice using real-time quantitativeRT-PCR assay. Based on the sequence of ESTs from the differential display, thefull-length genes were amplified using the RACE method.

实验方法：以生后第12天、第25天和第37天为研究时段，应用mRNA差异显示与cDNA表达阵列等方法分析rds小鼠发病过程中视网膜差别表达的基因，然后用Northern blotting和实时荧光定量PCR等方法，分析差别基因在rds小鼠和rd小鼠视网膜的表达，所分析的时间为小鼠整个生命周期。

Blotting out the sun along the shade of the Longhu Shinto temple, two tall with a thick wooden door with sound, the pleasant-sounding, just like the animal sound Jinji Phoenix, which is a well-known Rock King.

南岩宫 沿绿荫蔽日的神道进入龙虎殿，两扇高大厚实的木门开合有声，悦耳悦耳，正像金鸡凤凰的鸣叫声，这是南岩著名的一景。

Blotting out the sun along the shade of the Longhu Shinto temple, two tall with a thick wooden door with sound, the pleasant-sounding, just like the animal sound Jinji Phoenix, which is a well-known Rock King.

沿绿荫蔽日的神道进入龙虎殿，两扇高大厚实的木门开合有声，悦耳动听，宛如金鸡凤凰的鸣叫声，这是南岩闻名的一景。

Oxygen evolution , thermoluminescence , polyphasic rise of fluorescence transients and Western-blotting were measured, in order to study the effects of

结果显示：随盐浓度的增加，螺旋藻细胞光合放氧活性和PS II电子传递活性下降；TL B-band和Q-band强度降低，在0-0.6M NaCl下，B-band的周期性振荡清楚，最大值出现在第二次和第六次闪光，而在0.8M NaCl时，S态振荡基本上消失，S态氧化还原循环受阻；Fm， J、I和P相荧光水平降低。

The expression of apoptotic antibody Caspase-3 and PARP were increased by Western blotting.

经转染的DcR3-RNAi-SW480结肠癌细胞其DcR3 mRNA表达降低。

Clean air days" are counted in Beijing, and the official air-quality goal is "only three days of sun-blotting pollution each week.

北京的空气洁净日屈指可数，官方的空气质量目标是可怜的每周3天，其余的日子污染空气阻隔了阳光。

The sensitivity of chemiluminescent detection is very high, and it can be used to detect single copy gene in genomic Southern blotting hybridization.

化学发光技术检测敏感性高，可用于基因组Southern印迹杂交检测单拷贝基因。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。