查询词典 blotting

Recombinant bovine prion protein was treated by chemical solution, and the treated Recombinant bovine prion protein was intracerebrally inoculated in the brain. The prion gene expression was quantified and the proteinase resistant protein was detected by western-blotting.

对重组牛朊蛋白进行了化学处理后脑内接种金黄地鼠，200天后对朊蛋白抗性进行了western-blotting检测，对脑组织朊蛋白基因的表达进行了分析。

Western blotting shows that CTGF protein was over-expressed in neurofibroma tissue.

Western blotting结果显示神经纤维瘤组织有明显的CTGF蛋白表达条带。

Methods Specimens of neurofibroma tissue, keloid and normal skin were collected. The distribution and expression of CTGF was measured by immunohistochemistry. The mRNA expression of CTGF, TGF-β1,ColⅠ and ColⅢ was detected by reverse transcription- polymerase chain reaction. Expression of CTGF protein was measured by western blotting.

收集神经纤维瘤组织、瘢痕疙瘩组织及正常皮肤组织标本，免疫组化检测CTGF的表达，逆转录-聚合酶链式反应方法检测组织CTGF、TGF-β1、Ⅰ型胶原及Ⅲ型胶原 mRNA表达，Western blotting检测组织CTGF蛋白的表达。

In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection.

本研究采用RT-PCR和Western Blotting技术，检测正常培养和感染HCMV的神经胶质瘤（U251）细胞NGF的表达，探讨人巨细胞病毒对神经胶质瘤细胞内源性神经生长因子表达的影响。

Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

The effect of bavachin and corylin, two active principles from Psoralea corylifolia L., on UMR106 cyclin E expression by Western blotting was examined. The result showed that the two flavonoid compounds had no significant effect on cyclin E expression after various exposure time.

对于中药补骨脂中分离的两个促成骨样细胞增殖的活性成分补骨脂二氢黄酮和补骨脂异黄酮，采用Western blotting的方法考察不同时间对UMR106细胞周期蛋白cyclin E的表达，结果它们对cyclinE的表达没有显著影响。

Change its BL21 bacterium, with IPTG revulsive its convey corresponding protein, use Western Blotting test and verify.

将其转化BL21细菌，用IPTG诱导其表达相应的蛋白质，并用Western blotting验证。

Methods Mouse models of tibia fracture healing were established, and callus samples were collected 1, 3, 7, 14, 21 and 28 days after fracture. The development of callus and new bone formation were evaluated with roentgenology, Micro-CT and tetracycline double labeling method, and the expression of HIF-1α, vascular endothelial growth factor, Runx2 and ALP in callus were detected with RT-PCR, Western blotting and immunohistochemistry. The relationship between HIF-1α and fracture healing was analysed.

建立小鼠胫骨骨折模型，骨折后1、3、7、14、21、28 d取材，应用X线摄片、Micro-CT和四环素荧光双标记技术，观测骨痂组织的演变和新骨形成状况；采用RT-PCR、Western blotting和免疫组织化学方法，检测骨痂组织细胞HIF-1α、血管内皮生长因子和成骨性标志物（Runx2和ALP）的表达状况，分析HIF-1α与骨折愈合进程的关系。

At passage 3, cells were induced by 3 mmol/L sodium butyrate and 0.1% dimethyl sulfoxide for 5 days. Cells were harvested for Western blotting assay.

细胞培养到第3代时，分别用3 mmol/L丁酸钠盐与0.1% DMSO进行诱导，5 d后收集细胞进行Western blotting实验。

Methods SP-A and SP-D were determined in the pleural effusions from 36 patients with lung adenocarcinoma and 28 with tubercular pleurisy by western blotting.

方法收集36例原发性肺腺癌性和28例结核性胸腔积液患者的胸水，采用 western blotting检测胸水中肺表面活性物质相关蛋白-A、D。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐