查询词典 blotting

Asked for an explanation of the words on the blotting book Jones gave one readily enough.

被问及那个吸墨纸上的字迹的解释时，琼斯给了个非常肯定的答复。

The chair into which he allowed himself to fall was placed in front of that mirror, so fatal for him, so providential for Marius, in which he had read Cosette's reversed writing on the blotting book.

他倒在镜子前面的一把椅子上，这镜子对他是种不幸，但对马吕斯却是一种天赐，在镜中他见到了珂赛特吸墨纸上的反面字迹。

Some dESTs were labeled as probes to confirm mRNA in transformed and control cells by Northern Blotting assay.

我们将其中的部分dEST标记为探针，用Northern杂交法检测了相关基因在转化及对照细胞中的表达，证实了它们在差异显示凝胶中的差异表达结果。

Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

The bacteria containing pProEX-VP2-3-l was induced with Isopropyl-D-galactoside and samples of bacteria culture fluid were collected at different time respectively.

重组阳性菌用Isopropyl-D-galactoside诱导表达，收集不同培养时间的菌液进行SDS-PAGE电泳和Western blotting分析。

The bacteria containing the recombinant plasmids were induced with IPTG and samples of bacteria culture fluid were collected at different time respectively.

重组阳性菌用IPTG诱导表达，收集不同培养时间的菌液进行SDS-PAGE和Western blotting。

Objective To isolate,identify and purify the allergens in egg.Methods The albumen was extracted from the egg and the albumen from the vitellus.The total protein of the egg was isolated by SDS-PAGE.The allergens of egg were identified by Westernblotting.

分别提取鸡蛋的蛋清与蛋黄的蛋白，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离鸡蛋的蛋白质组份，采用免疫印迹(Western-blotting，WB)方法鉴定过敏原，通过离子交换层析对鸡蛋主要过敏原进行初步纯化。

Western blotting was performed.③Contractility: Carbachol (10-5 mol/L) treatment was conducted in selected SMCs for 30 minutes.

体外成血管功能：将等量的内皮细胞和筛选到的平滑肌细胞混合，在Matrigel上培养。

One hour later, the nuclear protein of the monocytes was extracted for electrophoretic mobility shift assay and the monocyte coverslips were collected for immunofluorescence staining for nf κb p65 subunit.

提取核蛋白进行nf κb的电泳移动迁移率改变试验；提取rna进行inos的rt pcr；提取总蛋白进行inos的western blotting；并留细胞爬片进行p65亚基的免疫荧光检测和inos的免疫酶学检测。

Methods MTT assay was used to detect the inhibition of the proliferation of K562 cell in different time and different dose. The effect of curcumine ramification with different dose in different time on P210(superscript bcr/abl) initiated signal pathway was detected by western blotting analysis.

四甲基偶氮唑蓝法检测姜黄素衍生物不同时间点和不同的浓度对K562细胞增殖的抑制作用。western blot的方法分析姜黄素衍生物FM17不同浓度、不同时间点对P210激活的信号的影响。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。