查询词典 blotting

Ansochromogenes was transformed by pUC1169 from itself. 8 mutants lost ability to produce nikkomycin were screened from 4068 colonies of Vio〓 Thio〓 with Tn4560 transposition. Southern blotting suggested that Tn4560 was inserted in at least 4 positions of chromosomal DNA in S.

于39℃，MM／Vio条件下培养携带有pUC1169的7100孢子，Tn4560发生转座；筛选到4068个转座菌落，并从中得到8株尼可霉素阻断突变株；对这8株突变株的总DNA进行Southern杂交分析表明，Tn4560至少在4个不同的位点插入到7100的染色体上。

Methods: Blood samples were taken from 40 RRTI children (26 males and 14 females, mean age of 3.68±1.47 years) and 35 normal children. Enzyme linked immunosorbent assay method was used to detect the levels of serum tIgE and Western blotting method was used to detect positive percentage of sIgE.

RRTI患儿40例，对照组健康儿童35例，采用酶联免疫吸附法检测血清tIgE水平，欧蒙印迹法检测血清吸入与食物组抗原sIgE抗体阳性率。

Rats were sacrificed at 0, 6, 12, 24 and 48 hours after reproduction of pancreatitis in both groups. The intestinal mucosal permeability was assessed with 99mTechnetium Diethlene Triamine Pentacetic Acid method, and the expression of substance P was evaluated with reverse transcriptase polymerase chain reaction and Western blotting analysis.

分别于制模成功后0、6、12、24和48 h处死两组大鼠，并留取标本；利用同位素法测定肠黏膜通透性，分别用逆转录-聚合酶链反应和蛋白质免疫印迹法检测P物质在肠组织中的mRNA和蛋白表达水平。

Methods The ES antigens of Trichinella spiralis muscle larvae cultured in vitro at 18 h and 30 h were analyzed by SDS-PAGE and Western blotting.

应用SDSPAGE和Western印迹对旋毛虫肌幼虫体外培养18、30h后的ES抗原中的蛋白组分进行研究。

Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress. There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedly raised for 24 hours, 4 days,7 days,14 days and 28 days respectively after SPS stimulation.The expressions of glucocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry,Western blotting and PCR, and image analysis and statistical analysis were performed.

成年健康雄性Wistar大鼠60只，使用无连续单一应激方法建立PTSD大鼠模型，分别取SPS处理后24h、4d、7d、14d和28d大鼠蓝斑组织，同时取正常蓝斑组织作为对照，应用尼氏染色观察蓝斑形态，采用免疫组织化学、免疫印迹和RT-PCR方法分别观察及检测各组蓝斑神经元GR表达的变化，并进行图像分析和统计学处理。

Methods Western blotting and immunohistochemical staining were used to detect CD147 expression in cervical cancer or normal cervix uteri tissues.

应用蛋白印迹及免疫组织化学方法检测CD147在宫颈癌和正常宫颈组织中的表达。

Methods Western blotting and immunohistochemical staining were used to detect CD147 expression in cervical cancer or normal cervix uteri tissues.

目的 探讨CD147在宫颈癌中的表达及其意义。

Key words: vernonia anthelmintica flavone; melanocyte; melanogenesis; western blotting

驱虫斑鸠菊黄酮；表皮黑素细胞；黑素合成；免疫印迹

We chose the gene, vomeronasal type 1 receptor(V1rc), as the first candidate gene and western blotting was employed to quantify the V1rc protein level in 0hr, 3.5hr, 7hr, 12hr after training.

我们挑选 vomeronasal type1 receptor (V1rc)做为第一个候选基因，以西方点墨法定量V1rc基因的蛋白质表达，分别记录在训练后立即、3.5小时、7小时以及12小时，四个牺牲时间点的V1rc表达量。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。