查询词典 blotting

Western blotting confirmed that the low activity of b-lactamase in E. coli Top10 pcs+ resulted from a lower amount of b-lactamase in its periplasm.

由此可见，周质内低含量的b-内酰胺酶是导致E.coli Top10 pcs+细菌氨苄青霉素抗性降低的原因。

Western blotting show that benzyl isothiocyanate and phenethyl isothiocyanate treatment gradually decreased the level of anti-apoptotic proteins, but increased the level of pro-apoptotic proteins.

接著，由西方点墨法的结果发现苯甲基异硫氰酸酯和苯乙基异硫氰酸酯会造成人类前列腺癌细胞株DU-145凋亡蛋白的增加和Caspase活性的上升。

Cosette on her arrival had placed her blotting-book on the sideboard in front of the mirror, and, utterly absorbed in her agony of grief, had forgotten it and left it there, without even observing that she had left it wide open, and open at precisely the page on which she had laid to dry the four lines which she had penned, and which she had given in charge of the young workman in the Rue Plumet.

珂赛特昨晚一到家，便把她的吸墨纸簿子放在碗橱上的镜子跟前，她当时正愁苦欲绝，也就把它丢在那里忘了，甚至没有注意到是她让它开着摊在那里的，并且摊开的那页，又恰巧是她在卜吕梅街写完那几行字以后用来吸干纸上墨汁的那一页。

Western blotting was used to determine the ex- pression of PTEN and relative proteins.

免疫印迹法检测 PTEN及相关蛋白在细胞株中的表达。

Three hundreds of five hundred fertilized eggs microinjected were transplanted into twenty nine pseudopregnant mice.

采取鼠尾，提取基因组。经PCR 检测，阳性鼠 22 只； Southern blotting 检测，阳性鼠 16 只，其中9 只母鼠，7 只公鼠。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

In chronic stress group, comparing with the control group body weight, weigh of testis and epididymis and number of spermatozoon of the rats were remarkable decreased (P.05), and the structure of somniferous tubule became thin, the cells in lumina for spermatogenesis became less and 3β-HSD expressed in rat testis reduced 19.8%.

HE染色显示睾丸内生精小管细小，管腔内生精细胞稀少，免疫组化显示3β-HSD表达降低，western blotting分析3β-HSD降低了19.8%。

Methods Twenty-four patients who underwent shoulder arthroscopy were included, and were divided into frozen shoulder group (n=12) and control group (n=12; n=2 for shoulder instability, n=5 for rotator cuff tear and n=5 for subacromial impingement).

以12例接受肩关节镜手术的冻结肩患者为研究对象，术中取肩袖间隙部位关节囊组织，Real-time PCR和Western blotting分别检测TFG-β、MMP1、MMP2、MMP3、MMP9、MMP12 mRNA和蛋白表达。

METHODS: The breast cancer cell lines Bcap37 were treated with different concentrations of vinblatine dissolved in dimethyl sulphoxide or caspase-3 inhibitor (DEVD-CHO, 100 μmol/L) for 3 h. The changes of the proliferation were detected by MTT methods. The apoptosis was determined by observing the internucleosomal DNA cleavage and PI staining, and the proteins of pro-caspase-3 and IκΒ-α were detected by Western blotting methods.

用二甲基亚砜对照、100μmol/L caspase-3抑制剂预处理乳腺癌Bcap37细胞3h后，加入不同浓度的长春碱，以MTT法检测肿瘤细胞增殖能力，以细胞DNA片段分析及PI染色法检测肿瘤细胞凋亡，以蛋白免疫印迹法检测pro-caspase-3和IκΒ-α蛋白的变化。

In vitro transcripts from RNA5 wild type clone were co-inoculated into Tetragonia expansa with the RNAs of Hu3 isolate containing RNA1, 2 and 3. Following RT-PCR and Northern blotting results shows RNA5 could be biologically replicated in the leaves. The virus concentration of the isolate containing RNA5 in T. expanxa was higher than that of the isolate Hu3 evaluated by ELISA detection.

野生型RNA5的体外转录物与含Hu3(RNA1+2+3)分离物的总RNA混合接种番杏叶片的RT-PCR和Northern blot检测结果都表明，野生型RNA5能够在接种寄主体内复制，并且ELISA检测证明含有RNA5的番杏病叶中病毒浓度高于不含RNA5病叶的病毒浓度。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。