查询词典 blotting

The recombinant proteins were purified according to the protocol of Ni-NTA Agarose and detected by SDS-PAGE, and TLC. Western blotting was used to identify their immunoreactivities with the serum of patient with clonorchiasis.

寄生性蠕虫以胞质GSTs为主，参与对由虫体代谢和宿主免疫系统所产生的脂质过氧化物和细胞毒性的羰基的解毒，对虫体的生存起重要的作用，是免疫疗法、化学疗法和免疫诊断潜在的靶标。

The confirmative tests of weak-reactive samples included neutralization assay for specific antigen and immune-blotting or immuno-fluorescence antibody assay for specific antibody.

确认方法可分为特异抗原确认的中和试验和特异抗体确认的免疫印迹或荧光抗体试验。

The main results are illustrated as following: 1. With the probe of the argB gene from Corynebacterium glutacium ATCC13032, it was made sure that the argB genes between C. crenatum A. S 1. 542 and C.

主要研究结果如下： 1 通过Southern Blotting实验，以谷氨酸棒杆菌ATCC 13032的argB基因为探针，确定钝齿棒杆菌A.S.1.542与谷氨酸棒杆菌ATCC 13032有较高的同源性。

ThenWestern blotting were applied to detect nuclear EGFR and cytoplastic EGFR in Caski cellswhich showed hight level expression of EGFR after radiation.

第四部分辐射对宫颈癌细胞EGFR核转运的影响EGFR家族成员直接转运入核调节靶蛋白和靶基因的表达和活性是新近发现的EGFR家族传递信号的新途径。

FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多；RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少，而Bax表达增加。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

The purified cTnⅠ was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

纯化的cTnⅠ采用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳和免疫印迹法进行鉴定。

LX2 cells were transfected at the adenovirus tite pfu100, and were harvested at different times (24,48,72h), then western blotting was executed to detected the expression of ectogenous ampk.

对凋亡信号通路的进一步研究发现，Bcl-2在LX2中是低表达的，而Bax在AMPK过表达之后，表达增加，而用RNAi方法敲低Bax之后，细胞再用携带AMPK基因的腺病毒感染，LX2细胞不再发生凋亡，说明Bax参与了AMPK诱导LX2凋亡的信号通路。

Western Blotting showed the gene was expressed specificly in the late stage of erythrocytic stage.

在恶性疟原虫体外抑制实验中，纯化的抗Pfpcp IgG不能有效的抑制两种虫株的生长。

These shoots were examined by PCR,PCR-Southem hybridization and DNA dot blotting.Results indicated that the geneencoding A1aB1b subunit of glycinin has been actually introduced into mulberry.

用PCR、PCR-Southem及DNA斑点杂交等方法对这些Km抗性苗进行了鉴定，结果证实大豆球蛋白基因确已被导入桑树体内。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。