查询词典 blotting

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

Dot and Southern blotting analysis of transgenic individuals resistant to herbicide Basta indicated that all of these individuals contained T-DNA insertion.

转基因植株分子杂交检测结果与Basta抗性筛选的结果一致，确定了转基因植株的Basta筛选浓度与时期，提供了有效、方便、经济的大规模筛选水稻转化后代的方法。

No, you know, they're blotting papers.

不，你知道，那是吸水纸。

Western blotting was used to detect protein expression of VEGF.

用免疫蛋白印迹法检测细胞VEGF蛋白表达。

The expression of p27kip1 protein was detected by Western blotting.

Western印迹检测p27kip1蛋白的表达。

The gene expression of c-myc was detected by RT-PCR and Western blotting respectively.

原癌基因c myc参与调控细胞的增殖、分化和凋亡过程，在多种恶性肿瘤细胞中都有很高的表达[1，2]。

Methods The expression of HRG-1 was analyzed with RT-PCR and Northern blotting.

方法用RT-PCR和Northern blot分析HRG-1的表达。

Western blotting analysis showed that most of biotinylated membrane proteins are belong to those of low abundance.

免疫印迹结果表明，生物素标记的膜蛋白基本上都是低丰度蛋白。

Once we detected a planet, we would have to find a way of blotting out the light from its star

一旦我们找到这样一颗行星，我们就要想办法将它的恒星射过来的光线遮暗

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。