查询词典 blotting

Using the obtained antiserum as the first antibody, and the monoclonal antibody 4C11 as the collator, the specificity of the antiserum was tested by Western blotting and indirect-ELISA with the Yak recombinant mature PrP, GST fusion protein, the extract of brain and the bacterium protein.

以自制抗血清为一抗，单抗4C11为对照，对牦牛重组成熟朊蛋白、GST蛋白、脑组织提取物和菌体蛋白进行Western blotting检测和间接ELISA试验，验证自制抗血清的特异性。

Western blotting showed less expression of cytochrome c in Hsp90 overexpression system than in control.

Western blotting检测Hsp90细胞中细胞色素c的表达与对照组相比明显减少。

Methods: Expression levels of MMP10 gene and protein in human spontaneous abortion decidua were determined by RT-PCR, Western blotting and distribution by immunohistochemistry.

利用RT-PCR和Western blotting技术分别检测MMP10基因和蛋白在人正常蜕膜和自然流产蜕膜的表达水平、利用免疫组织化学技术检测MMP10在人正常蜕膜和自然流产蜕膜组织中的分布。

It was a new mousegene in GenBank and named as SRG-L(spermatogenesis related gene expressed in the latestages of spermatogenic cells). The sequence of this cDNA has been deposited in GenBankwith the Accession No. AY352586. 2. Analyses of expression characteristics of SRG-L during mouse spermatogenesis.The results of RT-PCR and Northern Blotting indicated that no expression signal of thisgene could be observed in primitive type A spermatogonia and type B spermatogonia. Asignal first appeared in diplotene/pachytene spermatocytes, and was significantlyintensified in round spermatids and elongating spermatids, findly went down to beundetectable in mature spermatozoa.

RT-PCR和Northern Blotting分析表明：该基因在早期A型和B型精原细胞中不表达，从双线期初级精母细胞阶段开始表达并逐渐上调，在粗线期初级精母细胞、圆形精子细胞及长形精子细胞中均检测到高水平的mRNA，但在成熟精子中消失，具有明显的阶段特异性表达特征，并且组织特异性表达分析显示该基因在睾丸组织中的呈高表达。

The results of Western blotting and immunohistochemical method showed that HO-1 protein expression increased in DOX group and DOX -IR group,and was negative in normal group.

Western blotting法和免疫组织化学S-P法检测显示DOX 组和DOX-IR组 HO-1蛋白表达增加，HO-1在正常肝脏内的表达呈阴性。

Western-blotting confirms, but the shirt-sleeve albumen that dissolve conveys can have good immune reactivity with CCK-8 electropositive serum.

Western-blotting证实，可溶表达的融合蛋白能和CCK-8阳性血清具有良好的免疫反应性。

The expression of TGF-β in glomerulus was examined by immunohistochemistry. Smad2 phosphatase activity was detected by Western blotting.

光镜下观察肾组织的病理改变情况，免疫组化检测肾小球转化生长因子－β1，(TGF-β1)的表达，Western blotting检测Smad 2磷酸化水平。

Methods: Atrial tissue was obtained from the right appendage during open surgery in 25 patients with RHD. The mRNA of ACE and ERK2 and AT1R was semi-qualified by reverse transcription-ploymerase chain reaction and normalized to the glyceraldehyde 3-phosphate dehydrogenase. Western blotting analysis was employed to examine the expression of ACE and ERK and AT1R. Atrial collagen volume fraction was detected by Masson's stain.

25例风心病二尖瓣狭窄接受外科手术者，手术时取右心耳处心房组织500mg，通过逆转录—聚合酶链反应技术，以GAPDH为内参照测量ACE、AT1R、ERK2的 mRNA变化，用Western Blotting了解ACE、AT1R、ERK在蛋白水平表达的差异，经免疫组织化学了解胶原纤维容积分数改变情况。

In the paper, a preliminary study was made to analyze the interaction between hemocyanin, which was purified from shrimp P. vannamei obtained from Shantou, and anti -human antibody. The methods of Sephadex G-75 column chromatography, PAGE, Dot-ELISA, SDS-PAGE and Western-blotting were used.

以采自汕头的南美白对虾血清为研究对象，运用Sephdex-75柱层析、PAGE、Dot-ELISA、SDS-PAGE和Western-blotting等方法，探索血蓝蛋白与抗人IgG之间的相互作用。

The expression products were tested by Wester-blotting, it demonstrated the fusion proteins can be recognized by positive serum of hypoderma sp.

通过Westem·blotting分析表达产物，表明表达产物具有免疫活性，能被制备的阳性血清所识别。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐