查询词典 blotting

Methods Twenty-six NZW rabbits were randomly divided into three groups: a normal control group (fed on normal commercial rabbit diet, n =6), a hyperlipidemic group fed on high-fat ma total cholesterol (TC concentration at the beginning and the 12-week end point of the experiment determined,and all the experimental rabbits were sacrificed, and endothelial function test were performed on thoracic aortic rings prepared from the isolated aorta using the vascular endothelium-dependent relaxation parameter in response to acetylcholine The aortic atherosclerotic lesion expression of LOX-1 mRNA and protein were examined by RTPCR and Western blotting respectively.

方法新西兰大白兔随机分为正常对照组(普通饮食，6只)，高脂饮食组(高脂饮食，10只)及普罗布考组(高脂饮食+普罗布考200mg/kg·d，10只)。实验开始前及第12周分别耳缘静脉采血测定血清总胆固醇。第12周处死动物，取胸主动脉，制备离体胸主动脉环对乙酰胆碱的反应以检查内皮功能，RT-PCR与免疫印迹检测凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因与蛋白质表达水平。

The cloning, sequencing, homological blasting and Northern blotting results of 5 desiccation-induced cDNAs and 3 phosphate induced cDNAs implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as G protein, protein kinase, VP3and MAD3-like genes might be involved in dehydration in the resurrection plant Boea hygrometrica.

对其中5个脱水特异诱导表达的cDNA（包括可能与复苏能力有关的cDNA）和3个磷酸盐处理诱导表达的cDNA进行克隆测序、同源性探测和Northern杂交检测表明，牛耳草脱水过程中诱导表达的基因可能涉及到脱水胁迫的信号转导、调节基因的级联作用（VP3，MAD3样基因等）、结构基因产物调节细胞结构在脱水胁迫中的稳定性等。

Methods: The experimental g roup consisted of 24 patients with PE and 24 cases of normal placentae women respectively. Group of 24 patients with PE consisted of 12 mild cases and 12 severe cases. RT-PCR and Western Blotting were used to test the difference content of HLA-G between two groups.

采用半定量逆转录-聚合酶链技术及Western印迹技术分别检测了24例子痫前期患者(实验组，轻度子痫前期12例，重度子痫前期12例)，24例年龄相仿的正常晚期妊娠者胎盘组织中HLA-G的表达差异。

Tricine-SDS-PAGE and Western Blotting demonstrated that they could express ESAT-6 after heat induction either fused to BCG HSP60 or secretively into the culture filtrate, respectively.

Tricine-SDS-PAGE和免疫印迹证实这两株重组卡介苗经热诱导后分别能融合表达或分泌表达ESAT-6。

Eleven differentially exp ressed fragmentswere confirmed by bulked segregant analysis and reverse Northern blotting, further cloned and sequenced.

经过BSA 分析和反向Northern杂交鉴定，获得了11个差异表达的cDNA片段，并进行了克隆、测序和序列同源性检索分析。

The extinction of self is salvation; the annihilation of self is the condition of enlightenment; the blotting out of self is Nirvana.

灭绝的自我救赎；的自我毁灭的条件是启蒙；的印迹的自我涅磐。

METHODS: The expression level of HIF-1α and VEGF protein and mRNA were also assayed by western blotting and semiquantitative RT-PCR.

半定量RT-PCR检测HIF-1α、VEGF mRNA的表达，蛋白质印迹法检测HIF-1α和VEGF蛋白的表达。

Methods The different expression of CK in SHEE and SHEEmt cell lines,was observed by the immunocytochemistry and Western blotting.

采用免疫细胞化学染色和免疫印迹的方法，观察SHEE细胞和SHEEmt细胞中CK的表达。

Smirch of parts in a dry cleaning methods can be taken partial treatment: Using cotton ball or soft brush dip cleaning agent and gently scrub the stain to keep siphoning off the liquid with blotting paper, and then remove smirch dip the same way with water, washed off the excess reagent.

部件污点的干洗方法可采取局部治疗：使用棉球或浸软刷清洗剂，轻轻擦洗的污渍继续吸走了吸水纸液体，然后删除污点浸了水一样，水洗关闭多余的试剂。

For days on end, for the first two years of the famine, great clouds of these bugs swarmed over the four provinces, blotting out the sun and consuming almost all plant life in their path.

在饥荒的头两年，蝗群一来，有如乌云压境，遮天蔽日，席卷边区四省，数日不散，经过之地，庄稼片叶无存。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。