查询词典 blotting

Firstly,the presence and location of AAT in amphioxus was studied by Westernblotting and immunohistochemical methods.The results first revealed that AATpresent in amphioxus,a cephalochordate.Also it was demonstrated that AATdistributed in cellular plasma in hepatic diverticulum,the pouch that protrudesforward as an outpocketing of the digestive tube and extends along the right side ofthe posterior part of the pharynx,which has long been considered to be the precursorof vertebrate liver.In vertebrate,AAT is primary synthesized in liver.

我们首先利用免疫组化和Western blotting方法，研究了AAT在文昌鱼的组织表达和定位，首次在头索动物文昌鱼中证明了AAT的存在，结果显示，AAT定位于文昌鱼肝盲囊的细胞浆内，从AAT在文昌鱼中的合成部位来看，在功能上文昌鱼的肝盲囊等同于脊椎动物肝脏，肝盲囊是在发育分化过程中消化道组织向右前方突出沿着咽后部向前扩展和延伸形成的一个囊袋状结构。

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

Locations of transgenes in the recovered plasmids were determined by southern blotting. And then adjacent sequences of transgenes integration sites in three recovered aberrant classes were subcloned and analyzed. Transgene of A-2 was inserted into common carp DNA sequences homologous to the mouse phosphoglycerate kinase-1 gene and those of A-3 and A-6 were inserted into common carp DNA sequences homologous to the human epidermal keratin 14 gene and sequence of common carp β-actin gene, respectively.

通过Southern杂交对转植基因在回收质粒上的位置进行了定位分析，并对三种回收到的变异型转植基因旁侧顺序进行了亚克隆和顺序测定。A-2的转植基因整合到了与小鼠磷酸甘油酸盐激酶-1基因同源的鲤鱼基因组顺序旁侧，A-3的转植基因整合到了与人表皮角蛋白14基因同源的鲤鱼基因组顺序旁侧，A-6的转植基因整合到了普通鲤鱼β-actin基因顺序中。

Western blotting was applied to the phosphorylation of PKGⅡ substrate vasodilator-stimulated phosphoprotein to represent the activity of the kinase.

有报道指出，PKGⅠ和PKGⅡ在亚细胞定位、组织分化和功能上有所不同， PKGⅡ是膜结合酶而PKGⅠ是细胞溶质性酶［2，3］。

Determination of phosphorylation of c-Jun was performed by Western blotting and immunohistochemistry.

再灌注6 h的大鼠进行磷酸化c-Jun的免疫印迹和免疫组化检测。

The recombinant protein was purified with the different pH. Western blotting testing of the porcine expression of pGEMX POB in E.coli BL21 was positive.

利用非融合表达产品制备抗血清，检测融合表达的重组蛋白，Western-blot为阳性。

Objective:(1) To clone and characterize the Silent information regulator2 (SIR2) homolog from the parasitic protist Trichomonas vaginalis cDNA expression library, to express the fusion protein of TvSIR2 and TvSIR2-like/GST(isolated previously in our laboratory).

构建TvSIR2和TvSIR2-like基因原核表达重组载体并表达其融合蛋白，制备抗体，Western blotting鉴定后免疫荧光法进行细胞定位。

There was significantly difference between the expression of GR-α mRNA in pterygium and the normal conjunctivae.

Western blotting分析显示GR-α蛋白在翼状胬肉表达显著高于正常人的球结膜组织(P.01)。

Western blotting analysis revealed that the recombinant protein could react with rabbit anti-African swine fever virus VP73 polyclonal antibody. Fusion expression of African swine fever virus VP73L is helpful to to prepare ASFVserological diagnostic reagent in next work.

所得结果为进一步研究：分离纯化该重组融合蛋白；确证目标抗原蛋白VP73L免疫原性及其特异性，进而达到建立非洲猪瘟病毒的免疫检测方法奠定了必要的材料与技术基础。

Result: In total, 3264 fertilizated eggs were injected,1779 of which developedinto 2-cell embryos after in vitro cultured.The survival rate was 54.50%.1397survived embryos were reimplanted into the oviducts of psudopregnant kunbaifemale mice.After reimplantation,16 female mice were pregnant and 85 pups wereborn. PCR analysis indicated 11 out of 85 pups weretransgenic.SouthernBlotting detection showed 1 out of 11 was transgenic.

结果：共注射了3264个受精卵，继续培养，有1779个胚胎发育至2-细胞，存活率为54.50%，将1397个存活的胚胎移植到71只假孕昆白母鼠的输卵管内，共有16只移植母鼠怀孕，得到85只仔鼠，PCR检测表明11只为转基因阳性小鼠，对其中的部分小鼠进一步进行Southern Blotting检测显示，1只为转基因阳性鼠。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。