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By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

The molecular weights of the 11 major bands were 82, 79, 60, 51, 46, 38, 32, 30, 28, 24 and 18kD, respectively. The results of Western blotting with the positive serum of anaphylactic patients showed that the 1st-4th instar larvae all had the specific allergens whose molecular weights were 82 and 79kD, respectively; only the 5th instar larvae all had the specific allergen of 30kD. We purified the protein of 30kD by ion exchange chromatography and gel isolation and identified it as ectoblast protein by MALDI-TOF-MS.

选用家蚕过敏患者阳性血清进行免疫印迹，1~4龄家蚕均显示出82和79kD的特异性变应原；但只有5龄家蚕的30kD蛋白为特异性变应原，通过离子交换层析和经切胶纯化出30kD蛋白，再经MALDI-TOF-MS鉴定该蛋白为外膜蛋白。

Northern analysis indicated that expression efficiency of the bar gene driven by DCA1 promoter was regulated by the gradient concentration of sodium chloride, and the positive blotting signal intensity of the bar mRNA was highest in the medium containing 2 mol/L of sodium chloride.

Northern印迹分析表明：DCA1基因启动子驱动bar基因在杜氏盐藻细胞中的表达效率受氯化钠浓度梯度调控。

The transient correct expression of oleosin-hirudin fusion gene was identified by SDS/PAGE, western blotting and enterokinase treatment.

介绍oleosin的分类、分布、基本组成、结构与功能、oleosin基因及其表达，讨论oleosin在植物基因工程中的应用。

We found that rat Eppin was specifically expressed in the testis and epididymis.

为研究大鼠附睾蛋白酶抑制剂基因的表达，我们使用RT-PCR、Western blotting及免疫组织化学法检测了该基因在多种组织中的转录和蛋白翻译。

By immunohistochemical staining and western blotting,the expression of acetylcholine esterase was observed in the in mouse footpad sweat gland.

TPM是如何引起在儿童的泌汗障碍的，是否与对胆碱能系统的作用有关系？实验以此着手展开研究。

Flow cytometric also used for analysis the levels of reactive oxygen species, Ca2+ and mitochondrial membrane potential in both cell lines. The results showed Gyp promoted ROS and Ca2+ production and decreased the MMP level. Western blotting show that Gyp treatment gradually decreased the levels of anti-apoptotic protein (Bcl-2), but increased the levels of the pro-apoptotic protein.

除此之外，也藉由流式细胞仪分析测得经绞股蓝皂甘作用后的人类血癌细胞（HL-60）和小鼠血癌细胞（WEHI-3）里的活性氧化物浓度上升、钙离子释放以及粒线体膜电位下降等细胞凋亡重要指标，此结果可经由西方墨点法指出绞股蓝皂甘能使癌细胞中的抗凋亡蛋白Bcl-2表现量下降；促凋亡蛋白Bax表现量上升，同时也藉由共轭焦显微镜观察到致死蛋白AIF、Endo-G从粒线体释放至细胞核。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

Secretion of sAPPα and expression of PKCα were found to increase after cells were incubated with HupA, suggesting a possible correlation between these two changes.

Western blotting结果显示sAPPα和PKCα蛋白水平均随着与石杉碱甲孵育的时间增加而增高。sAPPα的显著上升出现于PKCα上升之前，提示石杉碱甲对sAPPα分泌的调节可能与提高PKCα表达水平有关。

Increases of APP transcript and expression by HupA also contribute to the up-regulation of sAPPα secretion. Meanwhile, Aβ secretion was observed to decrease 2 h after HupA treatment, and after 24 h incubation, HupA can significantly inhibit the secretion of Aβ.

Real-time PCR和Western blotting结果提示石杉碱甲能增加APP的转录和表达，该作用也将有助于对sAPPα分泌的上调；同时Aβ的生成并未相应增加，ELISA检测结果显示石杉碱甲孵育2 h后即能对Aβ分泌产生抑制作用，孵育24 h后能显著降低Aβ水平。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。