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Methods Three experimental groups of ROS17/2.8 cells were cultured in rotating clinostat for 24 h, 48 h and 72 h respectively, 1 h before the end of culture, BMP-2 (500 ng/ml) was added into the medium, and the expression of total ERK1/2 and phosphated-ERK1/2 (p-ERK1/2) were detected by Western Blotting method at th...

方法在地面1G和回转器模拟失重条件下培养ROS17/2·8细胞，培养时间分别为24h、48h和72h。

Molecular analysis by PCR and Southern blotting confirmed that the color patterns as expressed by cell colonies could represent the actual molecular events.

最后对部分克隆进行了PCR和DNA印迹鉴定，分子鉴定结果与发光的表型状况一致。

Results:Western blotting analysis showed that the product had apparent cross reaction with huMCP-1 monoclonal antibody.

结果：Western blot证明与huMCP-1单克隆抗体有明显交叉反应。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

The growth curve of cellular clone was determined by trypan blue exclusion test.

RT-PCR和Western Blotting检测PNP-TK基因在HepG2细胞中的表达。

Changes of P53, Caspase 3 and cytochrome C were detected by Western blotting.

药物对P53、Caspase 3，Cyto C等表达的影响检测采用免疫印迹法。

Key words:Western Blotting;13C urea breath test;Helicobacter pylori;Cytotoxin associated gene protein;Children

关键词］免疫印迹试验；13C 尿素呼气试验；幽门螺旋杆菌；细胞毒性基因相关蛋白；儿童

Flow cytometry was used to measure the cells cycle kinetics of the treated cells. Hoechst33258/PI double staining was performed to evaluate the cell morphology. Western blotting was conducted to analyze the expressions of p53, Bcl 2 and Bax. Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells.

以胎盼蓝活细胞拒染法测定药物对细胞生长、增殖的抑制及IC50 ；以流式细胞术观察药物对细胞周期的影响；以荧光双染法（Hoechst33258/PI）观察药物对细胞形态学的影响；以Western blot法观察药物对细胞P53、Bcl 2、Bax蛋白表达的影响。

Differential expression of the protein was demonstrated by Western blotting. The data indicated that the antibody was specific.

本研究制备的SOX4单克隆抗体具有良好的特异性，为进一步研究SOX4在肿瘤发生中的作用提供了重要的工具。

The differential expression level of nm23-H1 in the 2 groups of 5-8F cells were confirmed by Western blotting.

Western 印迹分析证实了nm23-H1在药物处理与未处理5-8F细胞中的差异表达水平。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。