查询词典 blotting

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to create diabetes. At 2 week after the models were created, BDNF protein was administered into the vitreous cavities of rats. At 4 week after the models were created, the rats were killed and the retina was removed for Western blotting and Whole-mount immunohistochemistry for TH to observe the changes of TH and dopaminergic amacrine cells in retina.

9周龄Wistar大鼠，链脲佐菌素（streptozotocin，STZ）腹腔注射，制成糖尿病模型，于模型建立2wk后，大鼠眼玻璃体内注射BDNF溶液，于模型建立4wk后，处死大鼠，取出眼球，取视网膜组织进行Western blotting及视网膜铺片免疫组织化学染色检测，观察视网膜中TH水平的变化，从而反映糖尿病大鼠视网膜中多巴胺能无长突细胞水平的变化。

Using immunohistochemistry and western blotting, to detect the expression of glucocorticoid receptor in the central amygdaloid nucleus of respective rat groups, and image analysis and statistical analysis were performed.

应用免疫组化、Western Blotting定位定量检测各组CeA的GR表达，进行图像分析和统计学处理。

ELISA and Western blotting showed that the recombinant protein has good antigenic.

ELISA和Western blotting实验证实，重组蛋白具有良好的抗原性。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

The carboxyhemoglobin levels in artery blood were detected with CO-oximeter and the expression of intercellular adhesion molecule-1 (ICAM-1) in the lung was detected by Western blotting.

应用一氧化碳血氧分析仪监测动脉血中碳氧血红蛋白水平的变化；应用Western blotting检测肺组织中细胞间粘附分子-1（ICAM-1）表达的变化。

The allergens with molecular mass of 21kD and 11kD were identified by Western-blotting. The 21kD and 11kD allergens of cashew nut were purified by ion exchange chromatography.

结果 腰果粗提液SDS-PAGE显示有10条蛋白条带，Western-blotting显示对腰果过敏患者的阳性混合血清能与其中2个蛋白条带起反应，分子量分别为21kD和11kD。

Methods The total protein of the cashew nut was isolated by SDS-PAGE. The allergens of cashew nut were identified by Western-blotting. To purify the allergens, ion exchange chromatography was employed. Results There were ten protein bands visible on the SDS-PAGE gel.

通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳电泳分离腰果的蛋白质组份，采用免疫印迹(Western-blotting)方法鉴定过敏原，通过离子交换层析对腰果主要过敏原进行初步纯化。

Western Blotting experiments shown that the CspA protein of Bacillus stearothermophilus can be expressed under the condition of not only cold shock but also the induction of Chloromycetin.

用纯化的蛋白质免疫兔子获得抗血清，Western Blotting表明CspA蛋白于嗜热脂肪芽孢杆菌中不仅冷激时表达，而且在受到氯霉素诱导时也会表达。

In Western blotting, Ig and Ig heavy chain of the sturgeons have the same antigenicity and can be detected by antibody of rabbit anti the sturgeon Ig respectively in colloxylin membrane while light chain cannot be detected.

Western blotting的检测结果表明，3种鲟鱼Ig的重链与其Ig具有同样的抗原性，在硝酸纤维素膜上可被兔抗鲟Ig多克隆抗体所识别，而轻链的Western blotting检测结果则呈阴性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐