查询词典 blot

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

The activation of signaling cascades was tested by Western blot.

Western印迹法检测信号通路的激活。

RT-PCR and Western blot were used to detect the gene/protein expression of cathepsin K.

用不同浓度的cathepsin K抑制剂E-64进行干预，油红染色评估效果。

This character is used in this study to develop three Western blot protein markers.

在本论文中，这种特点被用于制备三种western blot蛋白分子量标准。

The expressed product was purified by metal chelate chromatography, identified by Western blot and determined for activity.

表达产物经金属螯合层析柱纯化，并进行Western blot 分析及活性检测。

The expressed products were purified by Glutathion-Sepnarose chelation affinity chromatography and tested by western blot.

对表达的蛋白进行Western blot分析和谷胱甘肽琼脂糖亲和层析纯化，得到纯化的VP4融合蛋白。

Results: Only one blot is shown on each chemiluminescence map of the three proteins with low background.

结果：三种蛋白质的双向电泳化学发光图谱上均只出现了1个特异蛋白质斑点，信号强，背景低。

PCR, RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange.

PCR、RT-PCR和PCR Southern杂交结果表明：phyB基因已整合到枳橙的基因组，并得到了转录；转phyB基因枳橙表现出植株矮化、分枝角度加大、叶片变小、比叶干重增加，其叶绿素含量比对照高29.1％。

The results of PCR and western blot analysis demonstrated that cIX is broadly existing in various tissue of carp, when the tissue specificity is not distinct.

根据PCR以及西方墨点的实验结果，显示cIX是一个广泛存在於各个鲤鱼组织的蛋白质，并没有组织特异性。

OBJECTIVE: Western-blot technique is utilized to further validate FHL1 and HOXD13 gene expression in protein levels in muscle tissue of patients with congenital clubfoot, the in vivo HOXD13 and predicted binding sites in embryonic foot development was verified using chromatin immunoprecipitation technology.

目的：应用Western-blot技术进一步验证先天性马蹄内翻足患者足部肌肉组织中FHL1，HOXD13基因蛋白质水平的表达，应用染色质免疫沉淀技术验证在胚胎足部发育时在体内HOXD13和预测结合位点的结合作用。

According to our present broadcasting situation,the thesis proposes using new technology to update and reform the present HFC. It also suggests broadcasting using self-advantage and arranging EPON in order to win the supermarket.

论文从我国广电的现状出发，结合实际，积极倡导采用新技术更新、改造我国现有的HFC网络；倡导广电现阶段利用自身优势，部署EPON网络来赢取市场。

A new general kinetic equation was derived in which the effect of the removal of condensation water was taken into account completely, and was applied to the polyesterification kinetic study of AA/HPHP and AA/NPG. It was found that the reaction order of self-catalyzed polyesterification was not constant. For these polyesterifications, the reaction order was 2.5 at a low temperature, while 3.0 at a high temperature.

考虑缩合水的排除对反应体系的影响，用体积浓度单位推导出一个新的聚酯反应总包动力学方程，并应用到AA/HPHP和AA/NPG两个聚酯化反应的动力学研究中，研究发现，自催化聚酯反应的反应级数并不是固定值，对于AA/HPHP和AA/NPG两个聚酯化反应而言，低温时反应级数为2.5，高温时反应级数为3.0。