查询词典 blot up

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

The gene expression were obvious difference between lung cancer tissues and the para-neoplastic tissues,36 genes differential expression of lung squamous cell carcinoma were screened out,comprising 23 known genes, 13 unknown genes, among these genes, up and down regulated genes were 22 and Irrespectively.

Northern blot分析结果：对7条目的基因进行Northern blot分析后，我们首次发现金属硫蛋白一3基因(MT一3)魔在肺腺癌中明显高表达，MT一3在肺腺癌中的表达较在正常肺组织中的表达高5倍以上。

The expression of MDK was detected by real-time PCR and Western Blot. Results: The retroviral vector pLXSN-MDK was successfully constructed and infected SGC7901 cells,and the MDK gene expression of SGC7901/pLXSN-MDK was up-regulated.

病毒上清感染SGC7901细胞，G418筛选出阳性克隆，经实时定量PCR及Western Blot检测发现SGC7901细胞中MDK mRNA及蛋白表达水平明显上调。

The sponges can also be used not only for washing but also to blot up leftover water from the body.

该海绵也可以使用，不仅为洗衣机，但也污点了遗留下来的水从体内。

Western Blot analysis also demonstrated that level of ANXA1 in the villus was up-regulated.

Western Blot分析ANXA1蛋白质的表达，结果与RT-PCR一致。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated. Northern blot hybridization indicates that false ratio is high with the cDNA array experiment. Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated.

Custom cDNA array 筛选的8000个cDNA 克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调； Northern blot 实验表明cDNA array 的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST 序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

Through the guidance of professional, we knew to get Xian Jie area before evaporate, lest bilge hampers vapour permeates the skin; Want after clean face thin use currents and other characteristics of a river moist frost, lest heat up steam burnable skin; The hot water of 90 ℃ above is put in washbasin again after all these is finished, wrap up good hair, shut eye Fu body; Face and washbasin want apart 10 centimeters or so, if feel stivy, can look up with dry down towel gently blot is facial drip, rest moment changes hot water to continue again fuming or steaming-treating diseases with fumes as in moxibustion or with steam generated by boiling medicine herbs, relapse so 2-3 second, need about 15 minutes in all; After steam is fumed rinse in time with Wenshui, take a side with cold water, frost of nutrition of the Tu Xie after wiping.

经过专业人员的指导，我们知道了在蒸面前得先洁面，以免污垢妨碍蒸汽渗透皮肤；洁面后要薄施水性滋润霜，以免热汽灼伤皮肤；这一切完成后再在脸盆中放入90℃以上的热水，包扎好头发，闭目俯身；脸与脸盆要相距10厘米左右，如感到气闷，可抬头用干软毛巾轻轻吸干面部水珠，休息片刻再换热水继续熏蒸，如此反复2-3次，共需要大约15分钟；汽熏后及时用温水冲洗，以凉水拍面，擦干后涂些营养霜。

IHC, western-blot and immunofluorescence staning revealed that the levels of ILK and ICAM-1 up-regulated 6 h after RPE cells cultured with high glucose (30 mmol/L). Real time RT-PCR analysis showed that the levels of ILK mRNA increased at 2 h, and then decreased at 6 h.

免疫组化、western-blot和免疫荧光染色都显示RPE细胞在高糖培养6 h高表达ILK、ICAM-1蛋白，而实时荧光PCR检测显示在2 h其mRNA表达量开始上调，在6 h下降。

Western blot analysis with the antiserum ATP95 revealed a significant increase of protein amounts of the V-H〓-ATPase subunits B and c of S. slasa under 100 and 400 mmol/L NaCl treatment, which gave another evidence for a salt-induced coordinate up-regulation of V-H〓-ATPase subunits at transcription and translation levels. The coordinated salt-induced increase of subunit B, H and c of V-H〓-ATPase from S. salsa at transcription and translation levels indicated an increase of V-H〓-ATPase holoenzyme amounts, which might be the reason for the increase of V-H〓-ATPase activity of S. salsa under salt stress.

400 mmol／L NaCl处理盐地碱蓬植株分离其叶片液泡膜微囊进行Western-blot分析发现盐胁迫明显诱导了盐地碱蓬液泡膜H〓-ATPase B、c亚基蛋白的表达，证明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平存在协同表达。100、400mmol／L NaCl处理亦明显增加了盐地碱蓬叶片液泡膜微囊H〓-ATPase活性，表明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平的协同表达增加了H〓-ATPase全酶的数量，进而增大了H〓-ATPase的活性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐