查询词典 blot out

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

Trans-Blot Plus Cell 170-3990 Trans-Blot Plus Cell With Plate Electrodes and Super Cooling Coil, includes 3 gel holder cassettes, buffer tank, lid with power cables, 6 fiber pads, 1 pack blot absorbent filter paper (26.5 x 28 cm, 30 sheets), roller, stirbar 170-3991 Trans-Blot Plus Cell and PowerPac HC Power Supply Accessories 170-3994 Trans-Blot Plus Gel/Cassette Assembly Tray 170-3995 Fiber Pads, 27 x 28.5 cm, 2 170-3996 Blot Absorbent Filter Paper, 26.5 x 28 cm, 60 sheets 170-3997 Stirbar 170-3998 Trans-Blot Plus Roller, 6 wide 170-39 Trans-Blot Plus Gel Holder Cassette With Clamps 170-4990 Trans-Blot Plus Super Cooling Coil 170-4991 Trans-Blot Plus Platinum Anode Plate Electrode 170-4992 Trans-Blot Plus Stainless-Steel Cathode Plate Electrode 170-4995 Trans-Blot Plus Cell Buffer Tank 170-4996 Trans-Blot Plus Cell Lid With Cables 170-4997 Gel Holder Cassette Clamps, for Trans-Blot Plus cell, set of 3 9 规格:说明:运用Mini-PROTEAN II 多道筛选仪,可以快速有效地筛选 40 种不同的抗体或血清,无需把western

转印条件可调，能在很大的分子量跨度上获得最佳转印效果*耐用的板式电极能产生强大而均一的电场，而且电极在槽内的位置灵活可换；不论运行1、2 或3 个转印夹，电极间距可调节到最小以获得最大的场强和转印效率*凝胶支架转印夹保证整个凝胶与印迹膜表面的均衡接触*转印夹的铰链设计能防止凝胶滑动，便于转印夹组装*颜色标记的转印夹及电极板确保在转印槽内的正确定向*特级冷却芯和冷却水循环器进行温度调节―可理想地用于天然酶或高强度转印，或用于延长转印时间时减少缓冲液的损耗*可选择的组装盘是凝胶三明治和转印夹组装的理想选择 Trans-Blot Plus 转印槽能从单向和双向大格式凝胶上均一、高效地转移蛋白―配合使用新推出的 PowerPac HC 电源，多数蛋白都可在1530 分钟内完成转印。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Said you're going with your boys today and "Don't worry cause I'll be home late" But I know you're creepin' out there, cheatin', You've been freakin' babe So let me tell you how it's going to be And you can run game but not on me boy So who the hell are you trying to kid You know this is what I did I called your pager and your two-way boy but no response Start looking and I found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Baby boy I know you'll never find No stuff as good as mine She can't work you like I did You can look but you know I got that good shit I know that you're hating it But you better stay with the one you're with You made your bed and now you have to sleep in it baby I called your pager and your two-way boy but no response Start looking and found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door I ain't gonna take this no more I ain't gonna take this no more Bounce baby out the door Bounce baby out the door Get up and move Get up and move Don't make me act a fool Don't make me act a fool Just bounce baby out the door Wait a minute Sarah, give me a chance to explain I was just talking to shorty, I don't even know her name I ain't looking for no one to come and take your place Ain't no one out there baby that can fill your space No need to find a nickel got a dime at home Plus I love every night the freaky way we bone I ain't trying to get gone, ain't trying to roll I'd rather stay and be a part of your green eyed soul Just bounce baby out the door Bounce baby out the door Just bounce I ain't gonna take this no more Just bounce Bounce baby out the door Just bounce Get up and move Just bounce Don't make me act a fool Just bounce

我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待出去门弹起当作婴儿般对待出去门我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑假定你是去用你的男孩今天和"做不担心原因我希望在家迟"但是我知道你是creepin'出去那里cheatin'你已经是freakin 婴儿那样让我说出你，它怎样正将是和你能变得勇敢了但是不是在我上那样地狱对是你试一试的男孩哄骗你的知道这个是我所做的我呼吁你的寻呼和你的双向的男孩但是没有看和我反应出发发现你在情妇手臂不努力尝试的一些中我有我的自己眼睛的仅仅把厌烦你的我被的材料男孩原因带到那里听你所有的谎言处理前面的男孩原因你我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑小男孩我知道你希望从不裁决不材料实际已经开采矿她不能工作你相似我是做你罐看但是你知道我得到那个好排粪我知道那个你是讨厌它但是你更好些留下用一你是用你制做你的床和现在你富人向睡觉在中它当作婴儿般对待我打电话你的寻呼和你的双向地男孩但是没有反应开始看和发现你在中一些情妇's手臂做不请尝试向前男孩原因我拉来拉去你那里用我自己的眼睛仅仅到达你的材料男孩原因我被使厌烦的听见你所有的谎言弹起当作婴儿般对待从里面门我 ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门弹起当作婴儿般对待从里面门到达沿着和移动到达沿着和移动做不制造我行动a开玩笑做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起等候a分钟萨拉让步我a机会向说明我是仅仅说话向矮子我做不平稳知道她名字我ain't寻找没有任何人向来和发生作用你的地方Ain't没有任何人从里面那里当作婴儿般对待那个罐充满你的是一部分你的绿色是从从门公正弹起里面门公正弹起弹起小里面盯着精神公正弹起小我ain't去把这没有更公正弹起弹起小带到从门里面仅仅弹起组织和移动公正弹起不使我装出一傻瓜公正弹起的样子

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

Bounce [verse 1] I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Said you're going with your boys today and "Don't worry cause I'll be home late" But I know you're creepin' out there, cheatin', You've been freakin' babe So let me tell you how it's going to be And you can run game but not on me boy So who the hell are you trying to kid You know this is what I did I called your pager and your two-way boy but no response Start looking and I found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Baby boy I know you'll never find No stuff as good as mine She can't work you like I did You can look but you know I got that good shit I know that you're hating it But you better stay with the one you're with You made your bed and now you have to sleep in it baby I called your pager and your two-way boy but no response Start looking and found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door I ain't gonna take this no more I ain't gonna take this no more Bounce baby out the door Bounce baby out the door Get up and move Get up

弹跳 [韵文1 ] I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜刚刚反弹的婴儿出了门弹跳宝宝出了门 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜说你去你的男孩今天"不要担心，造成我会回家太晚"不过，我知道你在外面creepin '在那里， cheatin '，你已经freakin '贝比因此，让我告诉你如何这将是你可以运行游戏，而不是对我的男孩那么，谁该下地狱，你要多Kid 你知道这是什么，我我呼吁你的传呼机和你的双程男孩，但不作回应开始寻找，我发现你在其他一些妇女的怀里不要试图到前面的男孩引起我看到你，有我自己的眼睛刚刚得到你的东西男孩引起我已经厌倦了听你的谎言 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜刚刚反弹的婴儿出了门弹跳宝宝出了门 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜男婴我知道你永远也找不到没有任何东西一样好矿山她不能工作，你喜欢我你可以看看，但你知道，我还没好为Shit 我知道你恨它不过，你最好留一个您与你了你的床，现在你必须睡在它婴儿我呼吁你的传呼机和你的双程男孩，但不作回应开始寻找和发现，你在其他一些妇女的怀里不要试图到前面的男孩引起我看到你，有我自己的眼睛刚刚得到你的东西男孩引起我已经厌倦了听你的谎言弹跳宝宝出了门 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜刚刚反弹的婴儿出了门 I是在哪里也不会采取这种不 I是在哪里也不会采取这种不弹跳宝宝出了门弹跳宝宝出了门起床及动议起床

Methods The immunohistochemical method and Western blot method were used to determine expression of COX-2 protein in specimens of 47 cases (33 cases by IHC and 14 by Western Blot ) of nasopharyngeal carcinoma and 24 cases of normal nasopharyngitis tissues.

分别应用免疫组化方法和蛋白印迹方法检测47例鼻咽癌组织（其中用IHC33例，Western Blot14例）及24例鼻咽部炎症组织中COX-2蛋白的表达。

As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。