查询词典 blot out

By using the method of Western blot on fractions of nuclear and cytosol of primary cultured mantle cells and immunogold electron microscopy, we found out the completely different localization of these two proteins: CaM was localized both in cytoplasm and nuclear but CaLP was localized only in the cytoplasm.

通过对体外培养的珍珠贝外套膜上皮细胞核质分离后进行的Western blot分析和免疫电镜研究发现，CaM和CaLP的亚细胞定位是截然不同的：CaM分布于外套膜上皮细胞的细胞核与细胞质中，而CaLP却仅分布于外套膜上皮细胞的细胞质中。

The gene expression were obvious difference between lung cancer tissues and the para-neoplastic tissues,36 genes differential expression of lung squamous cell carcinoma were screened out,comprising 23 known genes, 13 unknown genes, among these genes, up and down regulated genes were 22 and Irrespectively.

Northern blot分析结果：对7条目的基因进行Northern blot分析后，我们首次发现金属硫蛋白一3基因(MT一3)魔在肺腺癌中明显高表达，MT一3在肺腺癌中的表达较在正常肺组织中的表达高5倍以上。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

TNFR1/RANK chimera cloning of cDNA, with its plat E-retroviral package by retroviral infection such transfer to TNFR1/R2 gene knock-out mice (TNFR1-/R2-) Bone marrow macrophages in; TNFɑ used to stimulate, respectively, to observe the OC and differentiation of bone re-absorption capacity; confirmed by Western Blot method TNFR1/RANK through NF-?

克隆TNFR1/RANK嵌合体的cDNA，用plat E将其包装成逆转录病毒，通过感染将这种逆转录病毒转染到敲除TNFR1/R2基因的小鼠(TNFR1-/R2-)骨髓巨噬细胞中；用TNFɑ刺激，分别观察OC的分化及骨的重吸收能力；用Western Blot方法证实TNFR1/RANK是通过NF-？

Last-minute, support searchs engine and live, the search engine regards as the stationmaster of parents of him back and belly, it is bubble is in free marsh cannot the person of extricate oneself, discharge wants free, the program wants free, the domain name also had better be free, should talk only receive fund, the fall out on the horse, but more acid is, want to search engine to change a bit a little only, or advertisement alliance money gives little, the clapperclaw that blot out the sky and cover up the earth began, as if the whole world is right only he is one.

归根究底，依靠搜索引擎而活，将搜索引擎当作自己衣食父母的站长，都是泡在免费沼泽里不能自拔的人，流量要免费的，程序要免费的，域名最好也是免费的，只要谈收钱，马上翻脸，但是更加讽刺的是，只要搜索引擎稍微有点变化，或者广告联盟钱给少了，铺天盖地的痛骂就开始了，仿佛全世界对的只有他一个。

Blood and CSF of patients were collected for syphilis,toxoplasma gondii and routine,cytological, glycose,protein,chloride, virology examination and 14-3-3 protein Western Blot of CSF were were carried out too.

采集患者血液和CSF，进行CSF常规及细胞学、糖、蛋白质和氯化物；血液和CSF病毒学、弓形体、梅毒检测；CSF 14-3-3蛋白Western Blot检测。2例死亡患者脑组织匀浆进行Wistar大鼠脑内接种，观察期6个月。

By southern blot and immunohistochemistry we find that Smad4 were efficiently knocked out in periotic vestibular cartilage capsule but not in vestibular sensory epithelium.

结果发现：1.Southern Blot及免疫组化显示小鼠内耳前庭软骨囊里Smad4被有效剔除，而前庭感觉上皮里Smad4表达没有受到明显影响。2。

In vivo, CXCR4-A549 and A549 cells were injected subcutaneously into nude mice(each group in 6).Four weeks later the mice were scarified and the tumors in situ and the lungs were taken out to be examined histologically.(3) Th cell cycle distribution was measured by flow cytometry to analyse the effect of SDF-1/CXCR4 in the cell proliferation and atoposis. The changes of the intracellular calcium concentration after SDF-1 activation were aslo measured by flow cytometry. Western blot was used to anlayse the phosphorylation of AKT and ERK, and the expression of MMP-2 and VEGF-C.

3为探讨SDF-1/CXCR4在肺癌侵袭和转移中的作用机制：①利用流式细胞仪检测CXCR4-A549与A549两组细胞的增殖和凋亡，分析上调CXCR4的表达后，对细胞生长周期的影响；②利用流式细胞仪检测两组细胞钙离子内流强度，分析SDF-1/CXCR4对钙离子通道活性的调控；③采用Western blot实验检测两组细胞PI3K/AKT、MAPK/ERK相关信号通路的活性，分析SDF-1/CXCR4可能激活的下游信号分子；④采用Western blot和RT-PCR检测两组细胞肿瘤转移相关基因VEGF-C、MMP-2的表达，探讨SDF-1/CXCR4与VEGF-C、MMP-2的相互作用关系。

Methods According to the examination result of vagina smears, 12 female rats were killed in proestrus, estrus, metaestrus and diestrus respectively, 3 for each, and their uterus and ovary were taken out, from which the total RNA and total protein were extracted for determination of transcription of SLC26A3 mRNA by RT-PCR, and expression of SLC26A3 protein by Western blot.

根据阴道涂片检测结果，将12只雌性Wister大鼠分别在动情前期、动情期、动情后期和动情间期断头处死，取出卵巢及子宫，提取总RNA及总蛋白，用RT-PCR及Western blot方法检测卵巢及子宫内SLC26A3在动情周期中各时期的表达。

Apoc., iii, 5:"and I will not blot out his name out of the book of life" cf.

载脂蛋白C ，三，五说：&我不会印迹出他的名字出书的生活&（见出埃及记32：33 ）。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失