查询词典 blood test

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

We collect the subjects' case history and use the detectors of vaseratm vs-1000 angiosclerosis to measure cavi,then deal with the data using statistical methods such as t test, partial correlation analysis and so on, to observe the effect of age, gender, disease and blood pressure on the cavi measure value.results: there are 167 cases male subjects and 374 cases female subjects.

收集病史，使用vaseratm vs-1000动脉硬化检测仪测量心踝指数（cardio ankle vascular index； cavi），采用t检验、偏相关分析等统计学方法进行数据处理，观察年龄、性别、疾病、血压对cavi测量值的影响。

The same operations were done on group D,but nothing were placed in the pocket and skin wound were then sutured.After an implantation period of six months,capsuler histological examination and blood silicone level test were performed.

术前测出血液中有机硅的浓度。6个月后取出假体观察，进行假体包膜组织学检查及大鼠血液中术后有机硅的浓度测定。

A 2-year-old male who had suffered from Hirschsprung's disease sine 2 months of age developed during 6 weeks bilateral fulminant exophthalmos with severe ecchymosis of eyelid and prominent congestion and chemosis of conjuntiva on the right eye and mild a degree on the left. A peripheral blood examination and liver function test showed mild abnormalities. A skull X-ray revealed increased bony density on the sphenoid ridge.

一位两岁男性病患，在2个月大时发现有Hirschsprung's disease，经过手术治疗，情形已改善，在2岁开始有右眼轻微凸出，经过6个星期，过程急速进展，演变成右眼严重凸出，眼睑严重瘀血，结膜充血水肿很厉害，角膜乾燥，左眼与右眼变化类似只是程度较轻微。

METHODS : Blood was drawn from six healthy volunteers into citrated test tubes.

从6个健康的志愿者采血，保存在柠檬酸盐试管中。

The test of white blood cell count was finished by the different number of bifidobacterium suspension coloclysis and general irradiation with 5.0 Gy γ ray to establish acute radiation damage pattern.The 30 survival rate was tested and reported after the bifidobacterium suspension coloclysis of 1×109CFU/ml and 5.0 Gy ray to establish acute radiation damage pattern number in every day.

WBC计数：用不同数量级浓度的双歧杆菌菌悬液对小鼠灌肠28 d后，5.0 Gy照射后3 d、6 d和10 d采取小鼠外周血检测WBC数。30 d小鼠生存率：用1×109CFU/m l双歧杆菌菌悬液灌肠28 d后，用一次性8.0 Gy60Coγ射线全身照射，建立急性放射损伤模型，每日上下午各观察小鼠1次，记录死亡动物数和死亡日期。

Element of acerbity kinase of flesh of the protein in fluid of lactic acid of the heartbeat that the author treated a swimmer in detail, blood, make water, serum, haemoglobin, hematuria and hematic Gao ketone / the test time of the 7 monitoring index such as coriaceous mellow ratio, method, note and its are opposite control its.

作者具体地论述了游泳运动员的心率、血乳酸、尿液中蛋白质、血清肌酸激酶、血红蛋白、血尿素以及血睾酮/皮质醇比值等7项监控指标的测试时间、方法、注重事项及其对控制其运动负荷的意义。

The micronucleus test has shown that cystamine has evident radioprotective effect at chromosome damage of blood lymphocyties and nucleated bone-marrow cells in irradiation rats. The Dose-Reduction Factor is 1.89 and 1.81 respectively.

微核测定法证明，胱胺对受照射大鼠的血液淋巴细胞和骨髓有核细胞染色体的辐射损伤有明显的保护作用，其剂量减低系数分别为1.89和1.81。

METHODS:Forty cases of traumatic optic neuropathy were analyzed retrospectively. Univariate analysis (Chi-square test) and logistic regression were performed to filter the risk factors to the visual prognosis of traumatic optic neuropathy. Eight variables were tested, including corticosteriod treatment in 2 days after trauma,no light perception,frontal or temple trauma,orbital hemorrhage and/or fracture,disturbance of consciousness,age ,other ocular trauma and presence of blood within the sphenoidal or ethmoidal sinus.

回顾分析40例外伤性视神经病变患者的临床资料，将伤后2d内是否接受激素治疗，伤后有无光感，有无意识障碍，是否有额颞部受伤，有无眶内积血和或眼眶骨折，患者年龄，是否合并其它眼外伤及是否有蝶、筛窦积血等8个变量纳入研究，通过单因素分析（χ 2检验）和多因素分析（Logistic回归分析），筛选出影响外伤性视神经病变视力预后的危险因素。

The hypercholesterolemic rats were selected to imitate early stage of human atherosclerosis The effects and mechanism of n-3 PUFA on endothelium were investigated with the following parameters: the morphological appearance and arrangement of endothelium stained by Ag, in vitro endothelium-dependentrelaxation of aortic ring, endothelial permeability test using Even′s Blue, blood lipid and body weight in one-week intervals, excitative and emissive spectrum of RBC membrane labeled by DPH (1, 6-Diphenyl-1, 3, 5-hexatriene), fluidity of RBC membrane, plasma and RBC membrane LPO

在整体和离体水平，选用大鼠高脂血症模型模拟动脉粥样硬化早期状况。用114只大鼠多指标着重研究n-3多不饱和脂肪酸在高脂血症状态下对内皮的保护作用，以探讨其预防动脉粥样硬化的机理。所采用指标包括：浸银法观察内皮细胞排列与形态；离体血管环观察内皮依赖性舒张；伊文氏蓝染料法观察内皮通透性。为探讨作用机理，进行了血脂和体重的动态监测、DPH(1，6-二苯基-1，3，5，乙三烯)标记红细胞膜的荧光光谱测定、红细胞膜流动性测定、血浆和红细胞膜脂质过氧化物测定。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。