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Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10% heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法,分离32例乳腺癌患者外周血中的单个核细胞,在6孔培养板上(106/ml,2ml/孔),用含10%热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

NT-3-ir cells belonged to the stroma cells of spleen and scattered mainly in the periarterial lymphoid sheath, lymphatic follicles and the inner wall of the blood vessels.

NT-3-ir 细胞疾病属于网状细胞疾病,主要散布于动脉周围淋巴鞘、淋巴小结和血管内壁。

Outcome:(1) Acute ischemic damage may induce the expression of c-fos protein in Cerebral Cortex quickly. Also, the expression increases by the ischemia time delay. It may hint that ischemia injury can rise the ability of nerve cells to tolerate ischemia and anoxia. And the expression level of c-fos can be up-regulated by putting blood of 12 hand Jing Points. So we think that treated by this therapy can rise the irritability of brain to resist the development of ischemia and enhance the ability to plerosis.(2) Acute ischemic damage may induce the expression of HspVO in Cerebral Cortex quickly. Also, the expression increases by the ischemia time delay. It may hint that ischemia injury can rise the ability of nerve cells to tolerate ischemia and anoxia.

三 结果(1)急性缺血性脑损伤可快速诱导c-fos蛋白在皮层缺血区脑组织的表达,其表达有随缺血时间延长而逐渐增多的趋势,提示脑的缺血性损伤可应激性地增强神经细胞对缺血、缺氧的耐受和适应能力,而应用井穴放血的干预治疗可明显上调缺血区皮层脑组织c-fos的表达水平,进一步增强脑组织对抗缺血性损伤的应激能力,抵抗缺血性脑损害的进一步发展。

Study on human and rabbit blood pressure, heart rate, myocardial enzyme, ESR and pathology index after ultrashort wave radiating pointed out that ultrashort wave of nothermogenesis and microthermogenesis dose had no injury on myocardial cells of human and rabbits, warmthermogenesis dose also had same effect to human, but not to rabbits. Ultrashort wave of thermogenesis dose radiating precordium for long time could seriously damage myocardial cells of human and rabbits, even bring out malignant arrhythmia and cardiac arrest.

超短波照射对新西兰白兔和人体血压、心率、心肌酶、血沉、病理组织学等指标影响的研究,说明无热量和微热量的超短波对兔和人体的心肌细胞无损伤作用;温热量超短波对兔心肌细胞有损伤作用,但对人体心肌细胞无损伤作用;长时间的热量超短波照射对心肌细胞有显著的损伤作用,甚至能导致严重的心律失常或心跳骤停。

Background— The Doppler Substudy of the randomized, double-blind, placebo-controlled Reinfusion of Enriched Progenitor Cells and Infarct Remodeling in Acute Myocardial Infarction trial aimed to inestigate the effects of intracoronary infusion of bone marrow–deried progenitor cells on coronary blood flow regulation in patients with reperfused acute myocardial infarction.

背景:应用多普勒,采用随机、双盲、安慰剂对照的方法研究输注浓缩祖细胞及梗死区结构的重塑,以明确经冠状动脉输注骨髓祖细胞对急性心梗患者冠状动脉血流恢复的影响。

Because of their shape, sickled cells clump together, especially in the smallest blood vessels, the blood becomes more viscous.

因为他们的形状, sickled细胞丛一起,特别是在最小的血管,血液变得更加粘稠。

Several sources may serve as reservoir of pluripotential stem cells.including bone marrow from a monozygotic twin(syngenetic trans-plant).a family member or unrelated donor with an identical human leukocyte antigentype.autologousbone marrow or peripheral blood.and more recently HAL-matched umbilical cord blood.

包括骨髓。从单双。家庭成员或无关供者与一个相同的人类白细胞抗原型。自体骨髓或外周血。以及最近的HAL匹配的脐血。单一的HLA不相合骨髓doner也造成了成功的移植。在较为有限sitiations 。

Methods: Mononuclear cells in umbilical blood and peripheral blood in normal pregnant women and unpregnant women were labled by erythrocyte specific antibody CD71 and fetal specific antibody HbF. Furthermore, NRBC were sorted by flow cytometry.

选用红细胞特异性抗体CD71、胎儿特异性抗体HbF对正常孕妇外周血、未孕妇女和胎儿脐血单个核细胞进行标记,并结合流式细胞仪对孕妇外周血中胎儿有核红细胞进行分选。

Freshly isolated blood mononuclear cells were reversely penetrated by unstimulated human umbilical vein endothelial cells and cultured in medium containing GM-CSF, IL-4 and TNF- a .

此外,本实验还用重组人CEA痘苗病毒转染DC,构建DC疫苗,以求在CEA阳性肿瘤患者的生物治疗上有所突破。

In peripheral blood,〓increases, so cause 〓 balance is broken, and 〓 which can induce inflammatory reaction increases obviously too; meanwhile, there are immuno- function change in the red cells, the RBC-〓 receptor rosette promoting rate of serum decrease, indicate this uveitic animal model have both cellular immunity dysfunction induced by T cells and the changes of RBC immuno-function. The analysis of the local and systemic immuno-function changes of uveitic animal model offers objective theoretical bases for combining the local treatment and the systemic treatment.

葡萄膜炎兔房水中房水中〓及IL-2均升高,病变葡萄膜组织中T细胞表面分化抗原的免疫组化检查显示大量阳性细胞,表明兔葡萄膜组织本身存在着免疫功能亢进所导致的病理改变,而外周血中〓增高,导致〓平衡失调,介导炎症反应的〓亦明显增高,同时伴有红细胞免疫功能改变,外周血中RBC-〓受体花环率及血清中RBC-〓受体花环促进率均降低,显示葡萄膜炎兔本身既存在T细胞介导的细胞免疫反应功能紊乱,也存在红细胞免疫功能改变,这种局部和全身的免疫功能改变分析为葡萄膜炎的治疗局部和全身相结合提供了客观的理论依据。

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