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Observe the influence of combination therapy with microwave ablationand peritumoral injection of immature dendritic cells on survival and tumor growth in mice.Observe clinical safety of combination therapy with MWA and adoptive immunotherapy of dendritic cells and eJector cells.Research the change of percentage of lymphocyte subsets and concentration of cytokine in peripheral blood after combination therapy.Initially observe the short-term result of combination therapy.

目的摸索微波消融治疗时有效灭活肿瘤细胞并释放肿瘤抗原的温控和能量条件;观察肝癌消融后局部注射未成熟树突状细胞对荷瘤小鼠生存期及肿瘤生长的影响;观察肝癌微波消融联合树突状细胞疫苗及效应细胞综合过继免疫治疗的临床安全性,研究微波联合细胞免疫治疗后近期外周血淋巴亚群比例及细胞因子浓度的变化,初步探讨细胞免疫治疗对肝癌微波消融后近期复发率的影响。

Methods: Nonadherent cells from bone marrow or peripheral blood mononuclear cells of 8 patients with chronic myeloid leukemia were cultured with rhGM- CSF and rhIL- 4. The cytokines were supplemented every three days. RhTNF-α was used at the ninth day. All the cells were harvested at the twelfth day.

从8例慢性髓性白血病患者的骨髓或外周血中获取非贴壁细胞,利用细胞因子(rhGM蛳CSF、rhIL蛳4、rhTNF蛳α)联合培养,每3 d补充新的细胞因子,其中rhTNF蛳α为第9天添加,第12天收获细胞。

Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient centrifugation. EGM-2 culture fluid was added, and then cells were plated on dishes coated with human fibronectin. After 48 h, the nonadherent cells were collected and replated on fibronectin-coated dishes.

采用Ficoll密度梯度离心法从人脐血中分离单个核细胞,加入EGM-2培养液,接种于包被人纤连蛋白的培养皿中贴壁培养,收集48h后的悬浮细胞重新贴壁培养至第7天,利用免疫荧光和流式细胞术鉴定。

On day40 after infection,donor derived Thy-1.1~+ CD8Tm cells were detectable in various organs including peripheral blood,spleen,lymph nodes,and liver.These cells were CD44~,CD62L~/CD62L~ in phenotype and more importantly,these cells were readily detectable for intracellular IFN-γsecretion several hours after ex vivo restimulation with OVA_(257-264) peptide.

感染40天后,可以在包括外周血、脾脏、淋巴结和肝脏在内的脏器中检测到一定比例的Thy-1.1~+ CD8 T细胞,这群细胞具有记忆性CD8T细胞的表型特征,即CD44~,CD62L~/CD62L~,更重要的是,这群细胞在体外OVA_(257-264)肽再刺激数小时后即可检测到细胞内IFN-γ分泌。

In nude mice, large and invasive tumors were found after injection of HuH7 cells subcutanesously. While mice injected with SeLT cells demonstrated highly vacuolated cells-filled tissue masses, severe necrosis, 4 inflammation as well as degenerated blood vessels.

利用皮下注射肝癌细胞株到裸鼠之后,实验发现HuH7 形成较大的肿瘤、有大量的血管形成且有转移的现象,而SeLT 虽然能够形成tissue mass 但其中大部分的细胞都有严重坏死及发炎的现象产生,伴随著新血管也呈现有萎缩的现象。

E. (P.001), which verified that after acupunctured head acu-points, the elas- ticity of the brain artery has been greatly improved and the tense brain artery is relaxed, the blood vessel dilatated, blood flow increased thus the condition of blood cireculation of patients has been improved. The brain tissue is well oxygenated, the brain cells around the focusis well nutriented, and finally speeds up the recovering of brain's function and the brain cell's metabolism.

验证了针刺头部腧穴治疗后能改善〓动脉的弹性,使其紧张度下降、血管扩张、血流量增加,从而改善了中风患者的脑部血液循环,提高了脑组织的氧分压,增加了病灶周围的脑细胞的营养,加速了中风患者大脑功能和脑细胞代谢的恢复。

Dopamine cannot be given directly because it doesn't cross the blood-brain barrier, the elaborate meshwork of fine blood vessels and cells that filters blood reaching the brain.

多巴胺不能因为它没有直接越过血脑屏障,细血管网的阐述和过滤血液,到达脑细胞。

Dopamine cannot be given directly because it doesn't cross the blood-brain barrier, the elaborate meshwork of fine blood vessels and cells that filters blood reaching the brain.

多巴胺不能给予直接,因为它并没有穿越血-脑屏障,阐述网状细血管和细胞过滤血液到达脑部。

Results We found that in all individuals'blood there are an amount of leucocytes parasitized by some moving-ac-tively tiny organisms,which even were found in some of the individuals'blood platelets.After dying we had observed the extracellular fluid of leucocytes and blood platelets.Analogically,in some plant juice and tissue cells we had also found the similar tiny organism,which had an ability to survive under the heat of90℃for30minutes.

结果 观察发现所有人血液中都能找到不同百分比的白细胞内有运动活泼的微小生物寄生,部分人血小板内也有,染色后还能观察到白细胞和血小板的外浆,在部分植物的汁液和细胞中也能看到类似的微小生物,这种微小生物还能耐受90℃高温30min以上。

Biochemical indicator such as TG and SF were unregulated while 1 system or 2 system reduced in peripheral blood; Bone marrow hyperplasia showed active or active obviously. Complete morphological nucleated cells mature erythrocytes or platelet hemophagocytic cells were found in 100% marrow slides. Marrow hemophagocytic cells were quantified as 0.5 to 11 per micro liter Median 4.2 per micro liter. Phagocytes showed smaller size single nucleus on one side. Submitting as rings cell plasm showed gray or salmon pink and cell showed disorder fringes.

结果: 符合HPS者30例诊断为HPS早期21例;HPS早期有持续高热峰值≥38.5 ℃、发热期≥4天发病与感染相关;以病毒感染最多见,生化指标TG增高、SF增高;外周血细胞一系或二系减低;骨髓增生活跃或明显活跃,骨髓片(100%)找到吞噬了完整形态的有核细胞、成熟红细胞或血小板的噬血细胞骨髓噬血细胞0.5-11个/ul中位数4.2个/ul吞噬细胞体积偏小多为单个核核偏一侧,可呈印戒样,胞浆灰兰或淡红色,细胞边缘不整齐。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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