查询词典 blood cells

There was an obvious increase of insulin receptor gene and protein in the cerebral ischemia reperfusion tissues in rats. The positive cells such as neuron, neuroglia cells, vascular endothelium cells possibly participated in regulating the processes of energy metabolism of cells and neovascularization. It implied that the high expressions of insulin receptors in vascular endothelium cells in ischemic zones possibly participated in regulating the pathophysiological changes of the blood vessels in ischemic zones; The higher expressions of insulin receptors in different cells were possibly one of the self-protective machanisms in which the ischemic tissues confront the anoxemia injury.

脑缺血再灌注大鼠脑组织胰岛素受体基因和蛋白水平表达增加；半暗带区神经元、部分神经胶质细胞及血管内皮细胞等阳染细胞可能参与脑缺血后组织细胞的能量代谢、促新生细胞形成等病理生理调节过程；胰岛素受体在缺血区血管内皮细胞高表达提示其可能在缺血区血管的病理生理变化中起某种调节作用；胰岛素受体在不同细胞中表达增高可能是缺血脑组织对抗缺血缺氧损伤的自身保护机制之一。

Now it is generally accepted that CD34〓 cells represent stem cells and progenitor cells. CD34〓 cells are better target cells for studying regulation of hemopoiesis, as they are relatively homogenous, compared with mononuclear cells seperated from bone marrow or blood.

目前普遍认为CD34〓细胞代表造血干细胞和祖细胞，因为与骨髓或血中分离的单个核细胞比较，具有相对的均一性，在造血调控的研究中是一个较好的靶细胞。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

The book provides reviews on many of the areas of stem cell, such as organ repair and regeneration; transcriptional regulation of blood stem cells; stem cells and tissue engineering; human fetal mesenchymal stem cells; gene-modified mesenchymal stem cells as cytoreagents for regenerative therapy ; pharmacological characterization of embryonic stem cell-derived cardiomyoctye cultures; adult stem cells in cardiac cellular transplantation; pancreas and liver regeneration and neogenesis; and stem cells and clinical application so on.

该书提供了干细胞许多领域的评论，例如器官修复和再生；血液干细胞的转录调控；干细胞和组织工程；人类胎儿间质干细胞；基因修饰的间质干细胞作为再生治疗的细胞试剂；胚胎干细胞来源的心脏肌细胞培养物的药理学特征；成熟干细胞在心脏细胞移植中的作用；胰腺和肝脏再生；以及干细胞及期临床应用等。

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达，16Hz、130dB次声作用1d组：两者在海马〓区和齿状回轻度表达，海马内血管轻度扩张；7d组：两者表达在上述区域较1d增多，微血管和小血管扩张较显，血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA；14d组：海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多，呈深兰色，在部分血管内皮细胞内两者强阳性表达，微小血管扩张状态较7d减轻；21d组：锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱；28d组：上述表达及分布与21d组相比无明显改变，海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达，血管扩张状态已明显减轻。90dB次声作用1d-7d后，较对照组无明显变化，14d-21d后轻度表达，28d后，Bcl-2mRNA和P53mRNA表达较强。

Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.

用粒－巨噬细胞集落刺激因子加白介素－4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡，将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养，同时设计不同类型肿瘤细胞（U937及培养胶质瘤细胞）作对照，分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e／macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡，将DCs、T淋巴细胞和凋亡胆管癌细胞共培养，同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照，7d后，分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Methods Hepatoma carcinoma cells and pulmonary carcinoma cells were cultured in vitro, Mononuclear cells in healthy person's peripheral blood were collected. PBMCs were mixed with liver cancer and lung cancer cells by proportion, and enriched tumor cells by superparamagnetic nanoparticle antibody complex, lung cancer specificness was identified by fluorescence nanocrystals bioprobe. We smeared tumor cells on glass slide and identified pulmonary carcinoma cells specfficity by fluorescence nanocrystals bioprobe.

体外培养人肝癌细胞和肺癌细胞，采集健康人外周血单个核细胞，将PBMC与肝癌细胞和肺癌细胞按比例混合，通过磁粒抗体复合物富集癌细胞，用荧光纳米晶探针进行肺癌特异性鉴定。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy（32/120; 26.7%）. The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders（22/120; 18.3%）. Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found（12/120; 10%）. Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters（8.3%）. Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters（4.2%）had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现，在总共搜集的120个病例中，其中因年老所导致的产精力不佳为最多，占38例（31.7%），於镜下可见大量精虫黏附於Sertoli cell的表面，并可见Sertoli cell数量明显增多而Leydig cell明显减少，且其细胞核呈现浓染的现象，而在其副睪中仍可见到成熟精虫蓄留於管腔中，但在其输精管内却只有少量精虫存在；其次为睪丸萎缩，占32例（26.7%），镜下可见中度至重度无造精作用，其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在，但Leydig cell数量明显减少且其细胞质内的脂质也明显减少；睪丸发育不全，占22例（18.3%），於生精小管内只见到精母细胞及少量Sertoli cell存在，不见造精细胞分化，於生精小管间质可见Leydig cell减少并伴随结缔组织增生；副睪结石，占12例（10%），肉眼下可在副睪及输精管前段见到黄白色大小不一的结节，触感坚硬，於镜下可见副睪管扩张并有大量成熟精虫蓄积，严重时可见钙化现象，以Von Kossa及茜素红染色均呈阳性反应；类淀粉沉著症，占10例（8.3%），镜下在睪丸间质及血管周围可见粉红均质样的物质沉积，以刚果红染色成阳性反应；马立克病，占5例（4.2%），镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润；睪丸坏死，占1例（0.8%），镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

Sichuan cord blood stem cells Centre Ltd is mainly engaged in cord blood collection, preparation, cryopreservation, genetic testing, clinical applications and related biotechnology development and utilization of the high-tech enterprises, under the "Sichuan cord blood stem cell bank" by the Ministry of Health Approved setting, which includes the functions of the public library and the library in two parts, the public library for the vast number of patients as a source of hematopoietic stem cell cord blood, cord blood stem cell transplantation since the body can be frozen cord blood for the needs of individuals and Family members provide services.

四川省脐血干细胞中心有限公司是主要从事脐血采集、制备、冻存，基因检测、临床应用及相关生物技术开发利用的高新技术企业，下属的&四川省脐带血造血干细胞库&由卫生部批准设置，该库的功能包括公共库和自体库两部分，公共库可为广大患者提供作为造血干细胞来源的脐血，进行脐血造血干细胞移植；自体库可为需要冻存脐血的个人及家庭成员提供服务。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。