查询词典 blastocyst

The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 ms, two) to reconstruct bovine cloned embryos.

以核移植胚胎的卵裂率、囊胚发育率作为检测指标，对不同的方法所获得的克隆胚胎的卵分裂率与囊胚发育率进行比较，最后筛选获得1个优化的牛体细胞核移植操作程序，即采用Spindle view系统对牛卵母细胞进行去核操作，将供核体细胞注射到卵周隙，然后通过电融合法将供体核引入去核卵细胞质(电融合参数为1.9 kV/cm，脉冲时程10 ms，方波2次间隔2 s)。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

The full developmental stages from preovulatory oocyte to expanded blastocyst of mouse embryos were observed by inverted DIC microscope, and photos were taken by digital microphotography.

在这些研究中，使用微分干涉差显微镜(differential interference contrast microscope，DIC)技术，对小鼠的早期胚进行细致的观察与操作是不可或缺的关键步骤［1］。

The full developmental stages from preovulatory oocyte to expanded blastocyst of mouse embryos were observed by inverted DIC microscope, and photos were taken by digital microphotography.

在这些研究中，使用微分干涉差显微镜(differential interference contrast microscope，DIC)技术，对小鼠的早期胚进行细致的观察和操作是不可或缺的关键步骤［1］。

Analysis of mitochondrial DNA composition of the panda-rabit cloned embryos found that mitochondria from both panda somatic cells and rabbit ooplasm co-existed in early blastocyst, but mitochondria from rabbit ooplasm decreased and those from panda donor cells dominated in early fetuses after implantation.

结果显示，应用卵裂球电融合方法可以制作一系列的多倍体胚胎；小鼠囊胚的形成与胚胎中的细胞数目无直接关系，卵裂球的电融合和染色体数目的增加不会改变胚胎的发育进程；而胚胎细胞中的核／质比可能是控制胚胎囊胚化的一个重要因素。

These ES cells were introduced into host embryos from outbred KMW though blastocyst injection. Using GFP, the attractive genetic marker, we have been able to follow the fate of ES cells in living embryos and to observe directly the contribution of these cells to mouse embryos. Totally, four GFP-expressing 'green' chimeric mice expressing GFP were obtained.

为研究嵌合体动物中供体胚胎干细胞在宿主胚胎发育中的走向和定位，同时探讨绿色荧光蛋白基因作为报告基因在转基因动物制作中的应用价值，本研究将pEGFP-N1基因导入小鼠ES-D3细胞系，得到稳定表达GFP的胚胎干细胞亚系ES-D3-GFP，通过对昆明小鼠的囊胚腔注射，获得了4只表达绿色荧光蛋白的嵌合体小鼠。

Methods PESC lines were derived from parthenogenetically activated blastocyst inner cell mass of different mice strains and cultured in different systems.

将小鼠MⅡ期卵子孤雌激活发育至囊胚，然后从囊胚内细胞团分离孤雌胚胎干细胞。

Penetration of sperm, pronuclear and blastocyst formation were observed.

结果 经改造的培养液HTFplus和IVF-30，体外受精率分别从13%、15%提升至70%、67%。

Even though the older oocytes were easier to activate and developoed more rapidly to the pronuclear stage than younger ones after artificial stimulation, the percentage of blastocyst development was lower than that in younger oocytes.

大龄卵比小龄卵易于激活而且形成原核的速度比小龄卵快，但卵激活后发育至囊胚的比例较小龄卵低。

After being transplanted into uterus of synchronously pseudopregnant mouse on pregnant D4, the embryo at blastocyst stage successfully implanted and normally developed onto term in the recipient uterus.

这些胚胎被移植到同步假孕小鼠子宫内后可以着床并发育到分娩。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。