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A novel fast recursive V-BLAST detection algorithm is proposed in this paper.

摘要该文提出了一种新型的快速递归V-BLAST算法。

Because many of these sequences are not in GenBank, a BLAST serer has been added; another new feature is an abbreiated alignment for the tRNA-like domain only.

由于其中的许多序列没有被GenBank收录,所以添加了一个Blast服务;新添加了许多来源于质粒的tmRNA序列,其中的5个是从公共数据库中收集而来,还有十条是直接测序得到的。

Methods The phage displayed antigens of paragonimus were used to examine the sera of patients with paragonimiasis,schistosomiasis,trichinosis and healthy people,and their sensitivity and specificity were acquired;The templates of the mimic antigen were purified to determine the sequence and the sequence homology was analyzed by Blast.

方法建立肺吸虫噬菌体模拟抗原的ELISA,对肺吸虫病人、日本血吸虫病人、旋毛虫病人及健康者血清进行检测,分析其敏感性和特异性;提纯噬菌体模拟肽的DNA模板进行序列测定,以Blast软件分析其序列同源性。

Methods the 32 variants of bartonella isolated in yunnan province and published in genbank from 1999 to today were taken as the target strains. the most closely related typical strains searched by blast in genbank with sequencing program were taken as comparative stains and their glta gene sequences were compared each other.

用云南省1999年至今分离到的并已在genbank中公布的32个巴尔通体变异体为目标株,利用网上核酸序列比对程序blast进行核酸序列同源性搜索,调出相似性最高且有效发表种典型菌株为对比株,进行比对和系统发育分析。

The results were the basic information to further study SNPs of MC1R gene associated with plumage color on mule duck. By using the program of Blast on GenBank, the results indicated that duck had homology with 98.4% homology with branta bernicla at nucleotide level of MC1R gene, and 92.8%~98.2% homology with other birds.

为进一步研究半番鸭羽色MC1R基因单核苷酸多态性奠定了基础;通过BLAST进行相似性搜索,结果表明,鸭与黑雁MC1R基因的核苷酸序列同源性最高为98.4%,与其他家禽的同源性也保持在92.8%~98.2%之间。

BLAST analysis showed that they have high homology with RGAs from other plants such as melon (Cucumis melo L.).

经BLAST分析表明,分离的南瓜RGAs与已报道的甜瓜等植物的RGAs有较高的同源性。

Afterward, the thesis introduces MIMO system primarily, then, based on the forenamed introduction, V-BLAST and STBC schemes are described systematically. Meanwhile, some correlated system block figures are provided to make understanding more convenient.

之后,对MIMO系统进行了初步的介绍,并在此基础上对V-BLAST和STBC两种方案进行了系统的介绍,同时给出相关的系统框图,更方便于读者的理解。

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料,采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库,初始文库滴度为3×10^6,插入片段平均长度大于1kb;随机挑取2004个阳性克隆进行5'EST测序,获得高质量序列1758条,经拼接获得1365条单基因簇,其中跨叠群175个和单条序列1195个;经ORF寻找共获得有效ORF1283条;经COG分析EST序列编码的蛋白质被分为22类;经Blast分析后,获得一批花色、花器官发育、花期调控以及其它育种价值基因(包括cDNA全长或片段)。

BLAST analysis indicated that it shared a high homological identity with other lipase from other plants.

BLAST分析表明,它与已报道的其他植物的脂肪酶基因具有较高的氨基酸序列同源性。

Sequences of modified genes GOX and CP4-EPSPS in GM canola and that of modified gene Cryla in GM cotton were decoded and the conservative sequences of exogenous resistant genes: PLRVrep, PVYcp and CryⅢA in GM potato: New leaf〓 PLUS and New leaf〓 Y were confirmed using the modified exogenous gene decoding technique and isogenous sequence similarity BLAST analysis. Bases on DNA sequences studied above, conventional PCR primers were designed and selected in an optimized way and the conventional PCR detection protocol for exogenous resistant genes in 19 GM crops was established.

本研究采用反向PCR克隆—测序测定未知序列技术,针对转基因玉米(MON810、BT11、BT176、GA21、T25、CBH-351)六个品系、转基因大豆GTS 40-3-2品系、转基因油菜RT73和MS8两个品系、转基因棉花MON531和MON1445两个品系测定出品系鉴定的边界序列;采用外源修饰基因序列的破译方法和BLAST同源性序列分析技术,针对转基因作物中修饰的外源抗性基因进行破译研究,破译出转基因油菜中修饰的GOX基因、修饰的CP4-EPSPS基因和转基因棉花中修饰的Cry1A基因的序列,并确定了转基因马铃署New leaf〓PLUS和New leaf〓Y两个品系中PLRVrep、PVYcp和CryⅢA外源抗性基因保守序列。

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