查询词典 biotin

Biotin is not saturated fatty acids required for the synthesis of the coenzyme acetyl CoA.

生物素是不饱和脂肪酸合成过程中所需的乙酰CoA的辅酶。

Methods The mouse embryo heads of different developmental stages of E10.5, E13.5, E14.5, E16.5, E18.5 and P0, P3, P6 after birth were acquired fixed with 4% paraformaldehyde for 48 hours, embeded with Paraffin and examined using LsAB （labelled streptavidin-biotin） method to observe the β-TrCP expression pattern in tooth germs, ameloblast and edontoblast.

方法取不同发育时期的鼠胚、乳鼠鼠头标本，用标记生物素链亲和素LsAB法观察β-TrCP蛋白在不同发育时期牙胚组织的表达情况。

Gonorrhoeae was amplified with the one set of primer for the 16S rRNA gene. The reverse primers were labeled with biotin. The products were identified by hybridization with its specific oligonucleotide probes labeled with amidogen in a nylon membrane.

选择NG 16S rRNA基因设计一对PCR引物，生物素标记下游引物扩增NG DNA，然后与固定在尼龙膜上的特异性寡核苷核探针杂交。

The chromosome composition of Yuan Zhong 2, a common wheat-Ag.intermedium partial amphiploid (2n=54), was analyzed with the genomic in situ hybridization,in which the biotinylated total DNA of Ag.intermedium as the probe and the total DNA of common wheat cv.Chinese Spring as blocking DNA were used to hybridize with the chromosome DNA of Yuan Zhong 2 at mitotic metaphase in root tip cells.

为分析普通小麦-天兰冰草部分双二倍体-远中2号(2n=54)的染色体构成，用生物素(biotin-16-dUTP)标记天兰冰草染色体组DNA作为探针，以普通小麦品种中国春染色体组DNA为封闭DNA，与远中2号的有丝分裂中期染色体DNA进行了分子原位杂交。

These patients, due to their medical condition, did not manufacture biotin in their gut and consumed no biotin orally or parenterally.

这些患者中，由于他们的医疗条件，没有生产生物在其肠道和消费没有生物口头或肠外。

Latest research indicates that,if eating a great deal of undone eggs for a long time,antibiotin protein in undone egg white can integrate with biotin internal the body as a sort of steady compound,making biotin can't be absorbed by intestinal wall and leading to energy ennui,muscle ache,hair falling off,skin inflamation,appetite letdown,weight droping and so on.When eggs pass through the intestinal tract,it's easy to zymolysis and transformation,sometimes it may appear nitroso compound,which has the function of causing cancer.

最新研究表明，若长期大量吃生鸡蛋，生鸡蛋清内的抗生物素蛋白与体内生物素结合成一种稳定的化合物，使生物素不能被肠壁吸收，则可导致精神倦怠、肌肉酸痛、毛发脱落、皮肤发炎、食欲减退、体重下降等；生鸡蛋经过肠道时，容易发酵变质，有时可能产生亚硝基化合物。

Different aspects associating the DMAE-NHS-based CLIA were studied in this thesis,including synthesis of acridinium ester,chemiluminescent characteristics of DMAE-NHS,labelling antibody or streptavidin with DMAE-NHS,two-site sandwich chemiluminescent immunoassay for TSH,two-site sandwich chemiluminescent immunoassay involved biotin-streptavidin system for TSH,and competitive chemiluminescent immunoassay using biotin-streptavidin system for TLVThe desired acridinium ester,DMAE-NHS,was synthesized according to the reference method with some modifications.

目前国内尚未见有关DMAE·NHS的合成及其应用于CLIA的研究报导，本论文研究了吖啶酯DMAE·NHS的有机合成方法，DMAE·NHS的发光性能，DMAE·NHS及其与抗体偶联物的热稳定性，DMAE·NHS标记TSH单克隆抗体和链亲和素，TSH CLIA，TSH BAS-CLIA和TT_4 BAS-CLIA。

BQJ-34 was cultured on the biotin fermentation media for 48 hour ,a 10ml fluid medium was boiled for an hour and was centrifuged (10,000r/min , 30 min), the supernatant is filleted with the 0.22μm filters and the biotin content of filter liquid is detected by RP-HPLC based on the biological samples regression analysis, recovery, accuracy testing.

利用生物素培养基对菌株BQJ-34进行振荡培养，取48h的细菌培养液10ml置100℃条件下水浴1 h，随后在超速离心机上以10 000 r/min，30 min离心，取上清液用0.22μm滤膜过滤，用高效液相检测滤液中的生物素含量，并通过对生物素样品的回归分析和回收率、精密度试验，最终确定利用反相高效液相色谱法可用于培养液中生物素测定，其最佳检测条件是：用安捷伦Zorbax SB-C18(4.6~※250cm，5μm)色谱柱，以K_2HPO_4(0.04mol，pH2.5)和CH_3CN(二者体积比为91.5：8.5)为流动相，紫外检测波长为215nm。

In the LAB, the solid phase first, and non-biotin labeled avidin reaction, and then coupled with biotin-labeled enzymes to further enhance the sensitivity.

在LAB中，固相生物素先与不标记的亲和素反应，然后再加酶标记的生物素以进一步提高敏感度。

In this paper ,the anodic differential pulse voltammetry has been applied to the research of the electrochemical behaviour of biotin,with gold electrode as working electrode.In solution of HAc NaAc(pH 3.59),the peak potential of biotin is 0.46V.The calibration curve is linear over the concentration range of 1.0×10 -6 ～2 0×10 -5 mol/L.

用微分脉冲阳极伏安法研究了生物素的电化学行为，用金电极作工作电极，在pH3.59的HAc－NaAc介质中，生物素的峰电位为0.46V，生物素浓度在1.0×10－6～2.0×10－5mol／L范围内与峰高呈线性关系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。