查询词典 biological method

In the present study, the new sensitive and specific LC/MS/MS method was developed for the simultaneous determination of magnesium lithospermate B, rosmarinic acid and lithospermic acid and their major metabolites in biological samples. The pharmacokinetics of S. miltiorrhiza depside salts in dogs, rats, and humans conducted the developed LC/MS/MS method, and they would be useful for new drug development and design.

在此基础上，系统研究了创新中药丹参多酚酸盐多个成分在Beagle犬及大鼠体内的药物代谢动力学特征，包括多剂量、多种给药方式的影响，及吸收、分布、代谢、排泄特征；同时进行人体药物代谢动力学研究，为设计合理给药方案和临床安全有效用药提供了理论依据，对传统中药的现代化研究具有显著的示范作用。

To study the cytopathogenic effect of mycoplasma membrane.the pro- toplasmic membrane of the mycoplasma causing swine enzootic pneumo- nia was prepared by the repeated Freeze-thaw method or the osmotic shock method,and was then inoculated into the rabbit kidney primary tissue cultures for examining their biological activities.The experimental results were as follows:in the cultures inoculated with 25～50μg of membrane protein ,the cytopathogenic effect was considerable.in the cul- tures inoculated with ...

用低渗解体及反复冻融裂解的方法，制备了猪喘气病病原霉形体膜，将所制备的膜制品接种兔肾原代单层细胞，作生物学活性观察，在接种量按蛋白含量计算为25～50微克膜蛋白的细胞管中观察致细胞病变作用，在接种17.5微克膜蛋白的细胞管中作用不明显，接种6微克膜蛋白的细胞管无致细胞病变作用。

The expressed product was purified by affinity chromatography and identified by Western blot, then determined for enzyme activity by Micrococcus lysodeikticus turbidimetric method and for biological activity by heat shock method.

表达产物经亲和层析纯化后，进行Western blot鉴定，并用溶壁微球菌比浊法检测酶活性，热激法检测其生物学活性。

The detection method comprises the steps that: conditions used for an Oxford cup method to determine polyxin B are established, standard solution of the polyxin B is used to draw a linear regression curve under the condition of biological detection, and then an Oxford cup is utilized to identify the size of a bacteriostatic ring produced by zymotic fluid at each stage, thereby measuring the content of the polyxin B in the zymotic fluid.

本发明公开了一种多氧霉素B快速定量分析的检测方法，该检测方法包括：建立牛津杯法测定多氧霉素B的条件，并用多氧霉素B的标准溶液在生物检测条件下绘制一条线性回归曲线，然后利用牛津杯鉴定各阶段的发酵液所产生的抑菌圈大小，从而测得多氧霉素B在发酵液中的含量。

Separation of them and synthesis, biological activities of their derivatives are of great signfication in development of advanced-processed products of resin.In this paper, Abietic acid with 90.9% and 97.5% purity were obtained using A amine salt method and B amine salt method for the first time.

本文提出两种提纯高纯度枞酸的新方法，即A胺盐法和B胺盐法，A胺和B胺均为自制品，通过A胺盐法和B胺盐法可分别得到纯度为90.9%和97.5%的高纯度枞酸，成本仅为文献报道常用的二戊胺盐法成本的1/5和1/3。

Based on morphological and biological studies, 36character transformation series of 10 species of the genu Apanteles Foerster and 2 species of the genu Dolichogenidea Viereck were recognized. Through analysis of character polarity using the method of outgroup analysis, two and multiple character data matrixe were obtained. The data is analysed through PHYLIP(Phylogeny Inference Package, version 3.6) software based on the method of cladistics, a majority rule consensus tree and a strict consensus tree constructed at last.

在本研究中，描述了10个种，做出了中国绒茧蜂属Apanteles Foerster种类雌蜂检索表，以绒茧蜂属的10个种作为内群，以长颊茧蜂属Dolichogenidea的2个种为外群，基于形态学和生物学分析建立了36个性状演系列，通过外群比较法，得到一个由二性状和多性状数据矩阵，再用phylip(系统发育推断软件3.6版)处理数据矩阵，最后得到一个多数规则合意树和一个严格合意树。

Its a practical method to classify pituitary adenoma with a combination of multi-measures: immunohistochemistry, ultrastructure and endocrinal characteristics; Nm23, Ki-67 and microvessel density play an important role in the biological behaviour of pituitary adenoma, and there exist co-relations between the three. The examination of the three helps to determine the biological behaviour of pituitary adenoma, as well as to supervise treatment and prognostic evaluation.

应用免疫组化激素检查、电镜超微结构观察并参考临床内分泌特征对垂体腺瘤进行形态和功能分类为具有实际应用价值的分类方法；nm23、Ki一67和MVD在垂体腺瘤的生物学行为中起重要作用，并且三者在垂体腺瘤中的表达有一定的相关性，三者联合表达的检测有助于判断垂体腺瘤的生物学行为及临床治疗和预后评估。

Four aspects of research achievements include:(1)several imaging correction methods and strained estimation methods for biological tissues elasticity imaging were investigated as well as elasticity reconstruction and phantom, in vitro and in vivo elasticity imaging experiments;(2)in the fields of biological microsystem and perfusion evaluation for microvascular system, three kinds of microbubbles whose shell are albumin, sugar ester and surfactant respectively were designed and produced, then the methods to estimate the thickness of their shells were presented, moreover, the interacting mechanism of ultrasound contrast agents and tissues, as well as protein drug delivery under the condition of ultrasound sonification were studied in the molecular level;(3) At the aspect of high intensity focused ultrasound therapy system, we designed and constructed the 256-element HIFU transducers using 1-3 composite materials. And furthermore we developed the integrated HIFU system with 256-element HIFU transducers. And we made preliminary and important progresses in the research of HIFU bioeffect, lesion estimation, techniques of monitoring and imaging and characterizing of structure of HIFU transducer;(4)The real-time method of voice source regeneration based on compensative regulation of physiological parameters was investigated in the field of medical rehabilitation. We addressed the physiology and biophysics of vocalization, modeled voice source regeneration based on compensative regulation mechanism, and put forward an integrated evaluation about the speech rehabilitation and the regenerated voice source; Sixty-one archived journal papers were published, as well as ten in international journals, and eighteen publications were embodied by SCI.

成果包括：（1）在生物组织弹性成像方面，研究了多种图像校正方法和应变估计方法，进行了弹性重构和仿体、离体及在体超声弹性成像实验研究；（2）在生物微系统和微血管血流灌注成像方面，制备了三种不同包膜超声造影微泡，研究了纳米包膜厚度检测与估计方法以及不同纳米包膜造影微泡断裂与药物释放机制，并在分子层次上开展了超声与生物组织相互作用机制以及蛋白质药物超声乳化微囊包裹的研究；（3）在阵列式高强聚焦超声治疗与监控成像技术方面，设计制作了新的HIFU换能器，解决了256阵元单焦点和多焦点合成HIFU治疗系统关键技术问题，并在HIFU生物效应与创伤性评价方法、HIFU过程监控成像技术和薄层复合结构与HIFU 换能器界面特性评价等方面的研究取得了初步而重要的进展，具有重要应用前景；（4）在语音听觉系统特别是嗓音康复方面，研究了基于发声系统补偿调节机制的实时多模式嗓音源再生方法和发声生理，建立基于神经、肌肉和空气动力系统的综合补偿调节模型，提出了相应的评价系统。

Four aspects of research achievements include:(1)several imaging correction methods and strained estimation methods for biological tissues elasticity imaging were investigated as well as elasticity reconstruction and phantom, in vitro and in vivo elasticity imaging experiments;(2)in the fields of biological microsystem and perfusion evaluation for microvascular system, three kinds of microbubbles whose shell are albumin, sugar ester and surfactant respectively were designed and produced, then the methods to estimate the thickness of their shells were presented, moreover, the interacting mechanism of ultrasound contrast agents and tissues, as well as protein drug delivery under the condition of ultrasound sonification were studied in the molecular level;(3) At the aspect of high intensity focused ultrasound therapy system, we designed and constructed the 256-element HIFU transducers using 1-3 composite materials. And furthermore we developed the integrated HIFU system with 256-element HIFU transducers. And we made preliminary and important progresses in the research of HIFU bioeffect, lesion estimation, techniques of monitoring and imaging and characterizing of structure of HIFU transducer;(4)The real-time method of voice source regeneration based on compensative regulation of physiological parameters was investigated in the field of medical rehabilitation. We addressed the physiology and biophysics of vocalization, modeled voice source regeneration based on compensative regulation mechanism, and put forward an integrated evaluation about the speech rehabilitation and the regenerated voice source; Sixty-one archived journal papers were published, as well as ten in international journals, and eighteen publications were embodied by SCI.

中文摘要：成果包括：（1）在生物组织弹性成像方面，研究了多种图像校正方法和应变估计方法，进行了弹性重构和仿体、离体及在体超声弹性成像实验研究；（2）在生物微系统和微血管血流灌注成像方面，制备了三种不同包膜超声造影微泡，研究了纳米包膜厚度检测与估计方法以及不同纳米包膜造影微泡断裂与药物释放机制，并在分子层次上开展了超声与生物组织相互作用机制以及蛋白质药物超声乳化微囊包裹的研究；（3）在阵列式高强聚焦超声治疗与监控成像技术方面，设计制作了新的HIFU换能器，解决了256阵元单焦点和多焦点合成HIFU治疗系统关键技术问题，并在HIFU生物效应与创伤性评价方法、HIFU过程监控成像技术和薄层复合结构与HIFU 换能器界面特性评价等方面的研究取得了初步而重要的进展，具有重要应用前景；（4）在语音听觉系统特别是嗓音康复方面，研究了基于发声系统补偿调节机制的实时多模式嗓音源再生方法和发声生理，建立基于神经、肌肉和空气动力系统的综合补偿调节模型，提出了相应的评价系统。

Biological degumming is a new developing way, it contribute to descending the fineness of bamboo fiber, and it endow fiber with tenderness , enduring grinding and enduring bending. So biological degumming is an advanced and green degummed method.

5生物脱胶作为一种新近发展的脱胶方法，对竹纤维的细度的减小有一定的贡献，而且它赋予纤维柔软、耐磨和耐弯曲的特点，是一种较先进的绿色脱胶方法，在竹纤维的制取过程中是一种较好的辅助脱胶手段。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。