查询词典 binding energy

The macrocyclic polyamines are a kind of excellent ligands and have a substantially high binding affinity with transition metal ions. Macrocyclic polyamine metal complexes as enzyme models are widely used in chemical biology, medicine and gene therapy. 1, 4, 7, 10-tetrazacyclododecane is a representative macrocyclic polyamines compound and shows high binding affinity with transition metal ions, such as Zn, Cu and Co. Their metal complexes especially multinuclear complexes were widely used in DNA, RNA recognition.

大环多胺化合物是一类性质独特的优秀配体，具有良好的金属配位能力，其金属配合物作为人工酶模型广泛应用于核酸结构及功能研究、基因表达与调控、抗癌药物的研制等生物、化学和医学领域。1，4，7，10-四氮杂十二烷是典型的大环多胺化合物，对过渡金属离子Zn， Cu and Co有良好的配位能力，这些金属配合物特别是多核金属配合物被广泛应用于DNA识别中。

Using 〓-SCH 23390 (0.02-2 nM) and 〓-spiperone (0.02-2 nM) as ligands bound to 〓 and 〓 receptors in rat neostriatum, Scatchard curve showed aproximately a linear relationship for both 〓-SCH 23390 and 〓-spiperone binding, indicating they labeled separate one binding site.

应用〓和〓〓与大鼠新纹状体中〓、〓受体相结合，Scatchard作图显示均接近直线，表明分别标记受体的一个结合位点。

The data display sharp resolution of binding position [+/-50 base pairs], which facilitated our finding motifs and allowed us to identify noncanonical NRSF-binding motifs.

数据显示了结合位点灵敏的分辨率(+/-50bp)，使得我们寻找motif 变得容易，并允许我们确定以前未发现的 NRSF-结合motifs。

Upon binding ATP or a nonhydrolyzable analog such as AMPPNP, RecA undergoes an allosteric transition into a high-affinity DNA-binding form. When RecA combines with dsDNA to form RecA-dsDNA nucleofiliment, it can unwind dsDNA and change the dsDNA structure.

在ATP或其类似物的辅助下，RecA可以直接与双股DNA结合而形成核丝，并将双股DNA加以解螺旋，并改变双股DNA的结构。

Results: 125 I Mel binding sites in optomeninx was the most, in eptochiasm and sniff ball was next; GTPγS dose dependently inhibited the binding.

结果 ：视网膜 1 2 5 I- Mel特异结合量最高，视交叉及嗅球次之，其次为下丘脑、海马、脑干；动力学分析表明 Mel与其受体的结合为可逆性结合；特异性结合分析提示对 Mel呈高度特异性；10和 5 0μm ol/ L GTPγS使 1 2 5 I- Mel特异结合降低。

Objective: To observe the effect of plasmid DNA binding to sarcoplasmic reticulum non-nuclear DNA binding proteins on SR function.

目的：观察DNA与骨骼肌肌质网蛋白结合后对肌质网功能的影响。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Component binding information was attached to intermediate schedulingresults. During scheduling infeasible binding configurations were removed according touser specified cost constraints, and multi-objective optimization was achieved byutilizing user specified cost function.

为中间调度结果附加组件绑定信息，并在调度过程中根据用户代价约束排除不合理&配置&、利用代价函数对多个代价目标进行优化。

Heat shock factor binding protein 1(HSBP1) is a nuclear-localized, novel, conserved, low molecular weight (00 residues) transcriptional factor, which may repress the activity of the heat shock factor 1 (HSF1) by binding HSF1 active trimerization domain.

热激因子结合蛋白1（heat shock factor binding protein 1， HSBP1）是一种新发现的高保守、低分子质量、定位于核中的一种转录因子，它可抑制HSF1的转录域活性，并协同HSP70负调控热激反应。

The Scatchard analysis of the competitive binding equlibrium indicates that there exists one strong binding site of Cu and Mn in HAS and BSA,which is at the tripeptide segment of N-terminal of protein.

竞争结合平衡的Scatchard图分析表明，生理pH条件下，Cu和Mn在HSA和BSA中均有一个共同的强结合位点，极可能位于蛋白质分子的N—端三肽段，Cu对此位点的结合能力大于Mn。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48