查询词典 binding course

In the titration experiment of NMR and IR spectroscopy, we can understand how sensors binding with metal ions: the two carbonyl group and crown ether group cooperated in binding with metal ions. Furthermore, by altering the "oxygen" atoms of crown ether to "sulfur", we can change the binding affinity to the soft metal: cupper. It shows 60-fold enhancement of fluorescence.

在化合物2中，我们引进了硫原子在冠状醚中，则应对电子密度较低之金属有较好的结合能力，实验结果显示了此分子对铜离子有相当好的选择性和结合能力，萤光增加可达60倍；同时由红外光吸收光谱和核磁共振光谱滴定实验可确知，香豆素衍生物上的两个羰基对金属离子的选择性及结合上扮演相当重要的角色。

For the former, the binding energy is small and the size is large, with the typical example being the deuteron. For the later, the binding energy is large. d〓 is a typical example, whose binding energy is hundreds of MeV, Even taking into account the theoretical errors, it is still a stable dibaryon candidate.

前者的束缚能较小，半经相对较大，典型代表是氟核d；后者有较深的束缚势阱，最典型的例子是d*，它的束缚能达到数百MeV，即使考虑到理论模型的误差，它仍然可能是紧束缚的双重子态。

Mutation of the gARID1 binding site in the cwp1 promoter resulted in a decrease of promoter activity and mRNA levels during vegetative growth and encystation, suggesting that the gARID1 binding sites are positive cis-acting elements of the cwp1 gene promoters in both trophozoites and encysting cells. Using cotransfection assay, we also found overexpession of gARID1 can increase luciferase activity and mRNA levels, while mutation of the gARID1 binding sites of the cwp1 promoter resulted loss of this transactivation.

我们也用萤光酵素基因接上cwp1基因启动子，如果将其cwp1启动子上的gARID1结合位置突变，也可以发现萤光酵素和讯息RNA的表现有明显的下；再用cotransfection assay，可发现gARID1会使萤光酵素和讯息RNA表现明显上升，然而如果将其cwp1启动子上的gARID1结合位置突变，这种转活化则消失。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The binding enthalpy change, the binding molar ratio and the binding equilibrium constant of harmine with hexamer are obtained.

而对d〓与潜在的抗肿瘤药物骆驼蓬碱的相互作用研究，已得到harmine与d〓结合的摩尔结合焓变，结合常数与结合摩尔比。

Atty acid binding proteins, a group of small molecular weight proteins with high binding affinities for fatty acids, are members of a superfamily of lipid-binding proteins, and occur intracellularly in vertebrates and invertebrates.

ABPs属于脂结合蛋白超家族成员，是一类分子量较小而对脂肪酸有高亲和力的蛋白质，广泛地存在于脊椎动物和非脊椎动物的细胞质中。

Were extracted by different extracting solvents in lab, the results indicated that with the increasing of Mn concentration levels, the speciation of Mn in their roots transform from H2O-extractable species to HCl-extractable species and NaCl-extractable species, showed increases of Mn of oxalate binding and pectate binding or prontein binding in cell wall of roots; Mn of H2O-extractable species were 34.55～50.26% of total Mn in stems and increased with the increasing of Mn concentration levels, which benefited upward transport of Mn; Percentage of Mn of H2O-extractable species were 38.55～44.19% and Mn of HCl-extractable species were 20.66～28.74% to total Mn in leaves, suggesting that most Mn in leaves were the speciation of H2O-solubility organic salts or manganese oxalates. 5. Chlorophyll in leaves of Polygonum hydropiper L.

不同的提取剂对水蓼根、茎、叶的提取结果表明，随着锰处理浓度增加，水蓼根中Mn由水提取态向盐酸提取态和氯化钠提取态转化，说明根细胞壁结合的草酸盐和果胶酸盐或蛋白质结合态的Mn含量随锰处理浓度的增加而增加；在茎中，水提取态锰含量占总锰的34.55-50.26%，且随锰处理的增加其水提取态锰的比例增加，这有利于锰向上运输；水蓼的叶片中，水提取态锰占总锰的38.55-44.19%，盐酸提取态锰占总锰的20.66-28.74%，说明叶片中的锰大部分以水溶性有机盐或草酸锰的形态存在。

The results show that this pyrano[4,3-b] pyran derivative observably quenches the fluorescence of BSA in a static quenching mode. The binding constants and the numbers of binding sites at different temperatures were obtained and the effects of some metal ions on the binding constant of the derivative and BSA were also discussed.

求得不同温度下二者的结合常数和结合位点数，探讨了微量金属离子对实验体系结合常数的影响，并根据热力学参数确定了这种吡喃并[4，3-b]吡喃衍生物与牛血清白蛋白之间的作用力类型。

Moreover, we have successfully applied this model to predict the relative binding free energies for four binding modes of EGFR/quinazoline. The most favorable binding mode identified by MM/PBSA could also be correctly recognized by MM/SAWSA.

我们把SAWSA替代PBSA计算用于结合自由能的计算，结果表明在计算效率大大提高的情况下，MM／SAWSA计算能得到和MM／PBSA计算可比的结果。

From the calculations, it can be found that the van der Waals interactions, the hydrophobic interactions, as well as the H-bonding interactions are crucial for the ligand binding. The 4-phenylamino group can produce strong van der Waals adn hydrophobic interactions with the nonpolar side chains of the residues deep in the binding cleft. The R^1 and R^2 substituents on the bicyclic chromophore can also produce strong van der Waals and hydrophobic interactions with the residues located at the exterior part of the binding pocket. Moreover, the two N atoms of the quinazoline can form H-bonds with EGFR, which will produce significant contribution to biological activities. The calculated nonbonded interactions between anilinoquinazolines and EGFR, as well as the information obtained from the predicted complexes, can interpret the structure-activities of the inhibitors well, which can afford us important information for structure-based drug design.

从模拟结果得到的抑制剂和靶酶之间的相互作用模式表明范德华相互作用、疏水相互作用以及氢键相互作用对抑制剂的活性都有重要的影响，抑制剂的苯胺部分位于活性口袋的底部，能够与受体残基的非极性侧链产生很强的范德华和疏水相互作用，抑制剂双环上的取代基团也能和活性口袋外部的部分残基形成一定的范德华和疏水性相互作用，而抑制剂喹唑啉环上的氮原子能和周围的残基形成较强的氢键相互作用，对抑制剂的活性有较大的影响，计算得到抑制剂和靶酶之间的非键相互作用能以及抑制剂和靶酶之间的相互作用信息能够很好地解释抑制剂活性和结构的关系，为全新抑制剂的设计提供了重要的结构信息。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48