查询词典 binding course

SDS-PAGE and Western blot of culture supernatant showed that hsIL-1RⅠ was secretively expressed. Recptor-ligand binding assays showed that the recombinant hsIL-1RⅠ has receptorligand binding activity.

发酵液经SDS-PAGE和Western blot证实分泌表达了sIL-1RI，经蛋白印迹受体配基结合反应证实分泌表达的sIL-1RI具有配基受体结合的生物学活性。

Upon initiation of PCR after the denaturation phase, the single-stranded DNA fragments flanked by inverted terminal repeats may form either self-annealing "pan-like" structures (preventing the primer binding to its complementary binding sites and suppressing the PCR) or DNA/primer hybrid structures.

经聚合酶链反应开始后，变性阶段，单链 DNA片段两侧末端反向重复序列可能两种自我退火"泛，如"结构（防止引物结合到其互补性结合位点和制止的PCR ）或DNA /引物混合结构。

The calculated amino acids sequence matches the known 30aa peptide sequence which shows high homology with some monocotylous mannose-binding lectin from Epipactis helloborine, Listera ovata and snowdrop . Such results shows that GAFP maybe a kind of new mannose-binding lectin of Gastrodia elata Bl.

根据GAFP与火烧兰、二月兰和雪花莲中的单子叶甘露糖结合凝集素的序列高度相似这一现象，我们可以推测GAFP可能是天麻中的一种甘露糖结合凝集素。

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关，一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力，FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现，目前已有很多的研究发现FIS与DNA结合的机转，然而这些实验的数据仅能显示推测性的结果，进而利用这些结果推论出FIS与DNA聚合体结构的模型，本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节，我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型，并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域，我们也分析FIS与DNA之间的氢键，并且与实验的资料作比较，最后我们计算FIS与DNA之间的结合自由能评估模拟的结果，并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

Ca2+ and EGTA-containing gel electrophoresis, combined with the corresponding terbium fluorescence on the basis of selective occupation of calcium binding sites, were applied to identify the potential calcium binding domains in PS II particles.

采用含Ca2+和含EGTA的凝胶电泳，结合竞争性取代钙结合位点的钙离子的铽荧光的方法检测光系统II中的钙结合蛋白。

According to the fluorescence measurement results from mixed solution, the association constant estimated for thioridazine hydrochloride-BSA binding and the number of the binding site with Scatchard analysis were 1.2×104 L/mol and 0.98, respectively.

将盐酸硫利达嗪与牛血清白蛋白以不同比例混合并在37 ℃下培养，以流动注射透析采样技术从混合液中分离出游离盐酸硫利达嗪，在线氧化为强荧光化合物后测定其浓度；应用Scrathard方程分析所测数据，求得盐酸硫利达嗪和牛血清白蛋白的结合常数为1.2×104 L/mol，结合位点数为0.98；根据热力学参数推断，盐酸硫利达嗪与牛血清白蛋白之间以疏水作用力为主；进一步基于荧光猝灭现象和Fster非辐射能量转移理论，得到盐酸硫利达嗪小分子与牛血清白蛋白之间的能量转移效率( E =0.497)和结合距离( r 0=3.27 nm)。

The fluorescence quenching of bovine serum albumin by thiouracil was investigated. The binding ratio between BSA and TU and the binding constant were found to be 1:1 and 1.35X 105 L/mol,respectively.

利用硫脲嘧啶对牛血清白蛋白内源荧光的猝灭，发现两者结合比为1∶1，结合常数为1.35×10~5L/mol。

The physical and chemical properties of DF from the white trumpet lily and tiger lily were analyzed to be water-binding capacity 4.1mL/g and 3.5mL/g, dilatability 6.5mL/g and 6.1mL/g, combining water capacity 7.1 g/g and 6.5g/g, exchangeable cation capacity 0.73 mmol/g and 0.83 mmol/g, and fat binding capacity 4.4mL/g and 5.2mL/g, respectively.

麝香百合和卷丹膳食纤维的理化特性分别是：持水力4.1mL／g和3.5mL／g，溶胀性6.5mL／g和6.1mL／g，结合水力7.1g／g和6.5g／g，阳离子交换能力0.73mmol／g和0.83mmol／g，结合脂肪能力4.4mL／g和5.2mL／g。

When we applied Trawler to data from yeast and mammals, 83% of the known binding sites were accurately called, often with other additional binding sites, providing hints of combinatorial input.

我们用Trawler对酵母和哺乳动物的数据进行分析，结果显示83%的已知结合位点被精确识别，而且有其他结合位点，提供了组合输入的暗示。

In addition, because these receptors have more binding points, they have better binding properties for anions than the molecular tweezer receptors based on thiourea we reported last time.

与以前我们报道的受体化合物相比，由于此类分子钳受体化合物具有更多的阴离子识别位点，因此具有更好地阴离子识别性能。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48