查询词典 binding clasp jaw

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

Each of these sequences is important for the binding of rna polymerase and initiation of rna transcriptio ...

本文综述了dna疫苗的意义，特点，作用机理及研制技术，并展望了dna疫苗的应用前景。

In this paper, based on the tight-binding Su-Schrieffer-Heeger model and by using a nonadiabatic molecular dynamic method, we investigated some unclear problems of polymers in details, such as polaron dynamic properties in different systems, the inelastic scattering processes of oppositely charged polarons driven by an external electric field,and the influence of electron-electron interactions on the dynamics of a charge polaron in conjugated polymers.

本论文基于一维紧束缚的Su-Schrieffer-Heeger模型，采用非绝热的分子动力学的方法，针对高分子聚合物中一些尚未清楚的问题，如极化子在不同体系中的动力学性质、正负极化子的非弹性散射过程，以及电子-电子相互作用对极化子动力学性质的影响等问题进行了较详细的研究。

In this paper, we have studied the binding energies of donors and the ground state energies of a polaron in a symmetric triangular quantum well.

本文主要研究了三角形量子阱系统中的类氢杂质态结合能和自由极化子基态能量的问题。

Study of the structure and function of the plasmin binding protein is in progress.

内皮细胞的接合体和文献中其他细胞接合体分子量类似，目前更进一步要研究此43kDa蛋白质的特性、结构及功能。

The specific receptor of plasmin was first studied by binding on the SDS-PAGE of the endothelial cell membrane.

因此细胞磷酸化程度的改变是经由何种机制仍待进一步研究。

It was demonstrated that the actin could be polymerized into discrete filaments of tree-like branch structure, random coil filaments cluster and long filaments with different diameters in F-buffer besides random aggregates. These polymerized filaments clearly exhibited the structural polymorphism and showed obvious difference from those assembled under the regulation by actin binding proteins or phalloidin.

研究发现，actin在体外通过自装配过程除了形成无序的蛋白堆积物之外，还能够聚合形成复杂的离散结构，包括树状分支的纤维丛、无规卷曲的纤维簇以及具有不同直径的长纤维等；这些大尺度纤维复合物明显不同于在ABPs或过量F-actin稳定剂参与下形成的由单根微丝和微丝束构成的聚合结构。

Monoclonal phages were picked randomly to detect receptor binding by ELISA assay, 80% of them c

三。重组人淋巴毒素突变体的特性鉴定

Northern blotting analysis indicated that 5 genes, nitrate transporter gene, nitrite reductase gene, ammonium transporter gene, ATP-binding cassette transporter gene, and purine permease gene, were significantly up-regulated under nitrogen starvation.

Northern blotting验证其中5个基因，包括硝酸盐转运蛋白基因、铁氧化还原蛋白亚硝酸还原酶基因、铵盐转运蛋白基因、结合ATP盒的转运蛋白基因和嘌呤透过酶基因，在缺氮诱导条件下转录水平有明显上调。

Ten of them were new sequences and the other 7 were duplicated sequences. These sequences represented respectively: depeptide ABC transporter periplasmic dipeptide-binding protein, permease protein, et al. Conclusion, Gene fragments specific to MTZ-resistant H.

对以上17个差异片段进行单向测序，根据最高同源性比对结果，分别代表二肽ABC载体渗透酶、二肽ABC载体结合蛋白等10个基因的同源基因片段在耐药株中存在高拷贝数。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48