查询词典 binding clasp jaw

Expression analysis with RT-PCR revealed that PcLGBP gene was exclusively expressed in hepatopancreas and the expression could be up-regulated by heat-killed Listonella anguillarum. The binding specificity of PcLGBP to ligand were subsequently examined by recombinant expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3) and ELISA approach.

为分析PcLGBP基因的功能，通过半定量RT-PCR方法研究了其在组织中的分布，并用荧光定量PCR技术分析了其对细菌刺激的响应，结果表明PcLGBP基因是肝胰腺特异表达的基因，能响应热致死鳗弧菌刺激而增强表达，说明PcLGBP基因可能参与螯虾的细菌防御反应。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

And in my opinion the female's high-heel shoes nowadays are the extension foot-binding culture of feudal dynasty.

女性的高跟鞋，我总觉得是封建裹脚文化的延伸

Highly conserved sequence from G46 to D104 existed in the middle of the translated a mino acid sequence, including an iron-binding motif (H3-D-H-L-V-C-L-D-C-G) with rich Histidines, No 'Fur-box' homology to the E.

在编码序列的上下游，RBS序列、-10序列、-35序列和二个潜在的转录终止子分别得到确定，但没有发现存在于大肠杆菌fur基因中的&铁盒子&。

In what way do these residues contribute to the strong binding of histones to DNA?

这些氨基酸残基是怎样为其与DNA的结合作出贡献的？

In what way do these residue s contribute to the strong binding of histones to DNA?

这些氨基酸残基是怎样为其与DNA的结合作出贡献的？

Protein phosphatases are crucial for cell living. Protein phosphatase 2A (PP2A) is an important member of serine/theronine protein phosphatase family, which is involved in almost all biological processes in eukaryotic cell. The 3D structure of PP2A core enzyme and holoenzyme was solved in 2006. These results are significant instructions for us to further understand PP2A structure, interactions between PP2A subunits, and also interactions between PP2A and its binding proteins.

蛋白磷酸酶在细胞的生命活动中起着十分重要的作用，蛋白磷酸酶2A(protein phosphatase 2A， PP2A)作为蛋白磷酸酶家族中十分重要的一员，它几乎与所有真核细胞的生命活动都有密不可分的关系。2006年，PP2A核心酶和全酶晶体结构的陆续破解对于深入了解PP2A自身的结构和亚基之间的相互作用，以及其与结合蛋白作用的机制都有重大的影响。

In this study, DRG-1 was identified to be a conserved gene in metazoans and share NF-κB binding sites with its homologues in their promoters.

在本实验中，我们发现DRG-1是一个在后生动物中保守的基因，而且在同源基因的启动子区域都含有一个NF-κB的结合位点。

Capillary electrophoresis frontal analysis and affinity CE methods were also developed to determine the unbound drug concentration and to investigate the protein-binding of clozapine and imipramine.

本研究还建立了CEFA和亲和毛细管电泳法测定游离药物浓度，研究了氯氮平和丙咪嗪等与蛋白的结合作用。

If this site changes then the virus will kill itself because it needs the exact structure of the IRES to remain the same for the binding of the ribosome.

要获得这样的对人体试验的许可是一个很长的申请过程。包刮要提供动物实验的数据，和毒理实验的数据。然后在合格的医生的严格操作下进行。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。