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PC12 cell ; subculture ; amitosis ; differentiation

PC12细胞；传代培养；无丝分裂；细胞分化

On the morphological differentiation,by inducing of fetal calf serum,it wasshown that the cytotrophoblast cells underwent aggregation first,and the adjacentcytoplasm membranes became tightly contacted through desomosomes andcytoskeleton.Then the adjacent cytoplasm membranes disintegrated fragmentallyto form fusion pore-like structure.Finally the multinuclear cells formed and inwhich the intercellular desmosomes disappeared.Immunocytochemical stainingfurther demonstrated that the formation of syncytium was through cell membranefusion rather than the amitosis process.

细胞形态分化的研究结果表明：早孕胎盘来源的细胞滋养层细胞在血清诱导下，首先发生聚集，并通过桥粒和细胞骨架使相邻的细胞膜紧密接触；之后细胞膜发生片段断裂，形成融合孔样结构；细胞间桥粒消失，最终形成多核的合体细胞；免疫细胞化学分析进一步证实合体化是通过细胞膜融合而非无丝分裂（amitosis即细胞核分裂而细胞质不分裂）实现的。

I'm gonna need a piece of this guy you got here in this Amman cell.

我需要个你从阿曼组织弄来的那样的家伙

The effect of pore structure in the anode catalyst layer on the performance of direct methanol fuel cell was investigated using ammonium carbonate as a pore former .

采用碳酸铵作为造孔剂，研究了阳极催化层孔结构对直接甲醇燃料电池性能的影响。

Objective: To investigate the possibility of amnion as cell scaffold in tissue engineering of temporomandibular joint.

目的：研究羊膜作为颞下颌关节组织工程支架材料应用的的可行性。

ObjectiveTo study a possibility of human amnion membrane as a substratum for culture of calf retinal glial cell .

目的 研究在具有完整基底膜的人羊膜上培养牛视网膜神经胶质细胞的可能性。

Limbus cornea stem cell autograft transplantation with fresh amnion is a safe and effective method.

自体角膜缘干细胞移植联合羊膜移植治疗复发性翼状胬肉是安全有效的治疗方法。

Objective: To evaluate the effect of limbus cornea stem cell autograft transplantation with fresh amnion in treatment of recurrent pterygium.

目的：观察自体角膜缘干细胞移植联合羊膜移植治疗复发性翼状胬肉的临床疗效。

We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚；(4)SDS-PAGE电泳发现，花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似，蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白，并且后两种蛋白几乎同时表达。

This is the standard used by cell phone companies, among others, to measure levels of radiation.

这是标准所使用的移动电话公司，除其他外，衡量的辐射水平。

London based fashion photographer Matt Irwin might be is the next big thing in fashion photography.

总部位于伦敦的时装摄影师马特欧文可能会是下一件大事的时装摄影。

Based on IMC system,the response would be better if the model matched more satisfactorily.

由于系统基于内模控制设计，故模型匹配度越高，系统响应越好。