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The preculture time of 2-4 days was beneficial to improve transformation frequency.Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium.The bacterial concentration of OD_(600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草莓叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD600nm值为0.4的菌液侵染10min效果最佳。

If Saritha wanted best out of Ramesh she should have given what is best for him.

如果萨莉莎想得到一个最好的拉梅什，她必须付出她最好的东西。

For them the best punishment is to do their best, I believe its own refueling ..

对他们最好的惩罚就是把自己做得最好，相信自己吧。。

"Of course, you don't want your goalkeeper to be the best player on the pitch, but it's reassuring that when he is called upon, Reina is the very best."

&当然，你不想你的门将成为球场最忙碌的人，但是需要他的时候，他必须可靠，雷纳往往是这方明的最佳。&

Through single factor experiment to seek the best carbon sources、nitrosen sources ,and orthogohal test to select the best conditions for producing floculants by strain Rhodococcus erythropolis.

通过单因素实验研究红平红球菌产絮凝剂的最佳碳源、氮源，并通过正交设计实验确定菌株红平红球菌产絮凝剂的最佳条件。

Speak of to why be fourth Lei be not being shown when hill is not roric alone alone medium he, xu Xin speaks of, we were done whole the research that made a trade, he should be a tertiary, but when I see Ding Lei, I say your company, he says us the first ah, I am best, although he is that, I feel he is not resembling of come to an agreement or understanding is to be being shown off, he is genuinely and sincerely feels he is best, I feel entrepreneur has this kind of mind also is very important, say you have so an ideal becomes soldier of industry file leader with this great ambition, I feel this is quite important.

谈到对于为何在丁磊不显山不露水时独独相中他，徐新谈到，我们做了整个做了行业的研究，他应该是排第三的，但是我见到丁磊的时候，我说你们公司，他说我们第一啊，我是最好的，虽然他那个，我觉得他并不是说好像是在炫耀，他是真心实意觉得他是最好的，我觉得企业家有这种精神也是很重要的，就说你有这么一个理想跟这个雄心去做行业排头兵，我觉得这个是挺重要的。

The nursery water was the best, when microencapsulated diets of "OK" and rotifera were blended at the percentage of 80% and 20%, the nursery water quality was the best during the rearing period, the highest concentration of NH3-Nt (NH3-Nm) was 1.025mg/L (0.0836mg/L),and the highest concentration of NO2-- N was 0.0236mg/L. When larvae emerged, the concentration of COD was 1.526mg/L, Puyrrop Hta was 1000 Entries/ml, and the metamorphic rates of all periods exceeded symmetrically 97%, and the survival rates also higher than the control group fed pure crude diets. The emergence status in P7 period was described as follows: the metamorphic rate: 73.33%; the average tail weight: 2mg; average body length: about 7.604mm; which were 18.30%, 19.3%, 8.4% higher than that of control group respectively.

其中80％欧开牌微胶囊饲料和20％卤虫配合使用的育苗效果最好，在整个育苗期间该实验组水质较好，NH_3-N_t(NH_3-Nm)含量最高为1.025mg／L(0.0836mg／L)，NO_2~--N_2含量最高为0.0236mg／L，出苗时COD为1.526mg／L，甲藻类含量为1000个／ml，幼体各期的变态率较稳定均衡，均大于97％，成活率也高于只投喂天然饵料的对照组，至仔虾第7天(P_7)出苗时，出苗率为73.33％，平均尾重为2mg，平均体长达7.604mm，分别比天然饵料的对照组高出18.30％、19.3％和8.4％，各项出苗指标均明显高于对照组(P＜0.05)；且虾苗活泼健壮、体表洁净、均匀度高。

The result indicated the black beans make black beans juice to be advantageous for the increase. And the best increase is in saccharify, the best recruitment is 8％, We can product black beans beer's original wheat juice density is 10.5％, total acid is 1.60mL/100mL,spirit content is 3.3%, the non-biological stability is good and the feeling in the mouth is neat, the taste is gentle.

结果表明，将黑豆制成黑豆汁便于添加，其最佳添加方式为糖化时添加，最佳添加量为8％，可制得总酸为1.60mL/100mL、原麦汁浓度为10.5％、酒精含量为3.3%的黑豆啤酒，非生物稳定性良好，口感清爽，口味柔和。

I have been very impressed with the leadership of Salian No.24. No.24 is the number one school in Dalian and this did not happen by accident. A school must have a strong leader to be the best in a city and Mr.Hou has shown to me that he is not only progressive,but that he cares very much about the students and strives to give them the very best education possible.

与此同时，这所学校的领导班子也给我留下了深刻的印象，大连24中学是大连市最好的高中，这绝不是偶然的，一个学校必须有一个领导有方的校长，才可能使学校成为全市最好的学校，24中的侯校长不仅有远见卓识，而且非常关心学生的成长，尽力给学生可能得到的最好的教育。

We've scoured the Internet and put together a list of what we feel represents the "best of the best" in web hosting companies today.

我们已经冲刷互联网和放在一起的名单，我们的感受代表了&最佳中的最佳&，在网页寄存公司今天。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新