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In partⅤof the study,the specific PCR detection method of dog cestodes was firstly established in China.When done specific PCR detection of dog cestodes,the best annealing temperature was 65℃and the best MgCl_2 concentration was 3.0 mM.

本研究的第五部分在国内首次建立了犬绦虫特异PCR检测方法，确定了对犬绦虫进行PCR检测时的最佳退火温度为65℃，最佳MgCl_2浓度为3.0mM，此时，检测条带亮且没有非特异性条带出现。

I cannot choose the best.the best choses me.

我不能选择最好的。最好的选择我。

Modeling functions for seven higher alcohols and six esters in cider were established,which have major and decisive contributions to the flavor of cider.5~# was selected out of eight ciders as the best one by fuzzy comprehensive evaluation methods,and the next best was 6~#.

长期以来，国内外的生产和科研工作者们普遍采用简单根据感官品评的方法去评判苹果酒质量的优劣，由于评酒人员感官上存在一定的生理差别和个人喜好的不同，导致某些酒样的部分重要信息被掩盖[2]。

Unfortunately, the best game designers are usually not the best coders.

不幸的是最好的游戏设计人员往往不是最好的程序员

The American Royal Rodeo invites the best cowboys and cowgirls from across the country to participate in its competition. Plus, there's a barbecue cook-off, featuring some of Kansas City's best grillmasters.

全美各地的最佳牛仔及女牛仔，都会受邀参与美国第一等牛仔竞技会的竞赛项目；除此之外，现场也会举办烤肉烹饪大赛，以堪萨斯市当地一些技艺出超的烤肉大师做为号召。

It dishonours the long series of Englishmen who during the past hundred years have gladly taken over what was best, and not only what was best, in German thought.

它污辱了为数众多的一系列英国思想家，他们在过去100年中心悦诚服地接受了德国思想中最好的、而不仅只是最好的东西。

Jimmy was not only one of the world's best drummers, but more importantly he was our best friend and brother.

吉米不仅是世界上最好的鼓手之一，但更重要的是他是我们最好的朋友和兄弟。

In my opinion, he's got one of the four or five best fastballs in the big leagues, the best slider in the big leagues, and he's got a good curveball and changeup and he's got good command.

&我认为他是大联盟中4到5位最好的速球投手之一，他有大联盟中最好的滑球，突出的滑球和变速球，他的控球也很稳。&

"Mayweather's fighting one of the best in Juan Manuel Marquez but he's not fighting the best in his weight class," Mosley said. If he can't come down a few pounds to meet Marquez halfway that means he's a full-bodied welterweight.

&梅威瑟对阵最棒的拳手之一----马奎兹，却不去挑战他那个级别的王牌，&莫斯利说，如果他不杀掉几磅体重，即在两人体重级别的中间独斗马奎兹，那他就是彻头彻尾的次中量级拳手。

He wants the best for his family, which can best be displayed by his fleet-footed returns home when his wife calls in the latest emergency.

他想要给他家人最好的，从他太太只要一打电话说有紧急事件他就会马上飞奔回家这件事情就看得出来。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。