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This article consists of a three-chapter text, a brief instruction and an epilogue: achievements of the research on bel canto and thefolk singing as well as their relation in China were concluded briefly in the introduction. In chapter 1, the origin and characteristicsof bel canto and the folk singing, together with their fusion processin present China was illustratively revealed though historical review. In chapter 2, bel canto and the folk singing are analyzed in technical aspects like breath, vocalization, pronunciation, and resonancetogether with comparison on their similarities and differences.; Chapter three is concerned with the application of bel canto in folk singing and nationalization of bel canto as well as the feasibility oftheir fusion from technical aspect. Moreover, it is illustrated thatthe fusion of these two vocal arts in china not only conform to social development and mass aesthetic requirement, but also has great influence on the education and development of Chinese vocal arts. Epilogue is to express wishes to future Chinese vocal arts based on the trend of multi-culture fusion and innovation.

本文共由三章正文和简短的绪论及结语构成:绪论粗略总结国内关于美声唱法、民族唱法及二者关系的研究已有的成果;第一章尝试通过历史回顾直观展现美声唱法、民族唱法之由来、特性及二者在当代中国对话融合之进程;第二章侧重从呼吸、发声、吐字、共鸣等技巧层面分析美声唱法、民族唱法各自的技巧特点并比较二者之间的异同;第三章除着重探讨美声唱法的民族化、民族唱法的美声化及两者融合共促于技术层面之可行性外,更试图说明此二种声乐艺术的融合发展在中国当前不仅是符合社会发展趋势和大众审美要求,更将对中国声乐艺术的教育和发展具有重要影响;结语则就多元文化融合创新之大趋势表达对未来中国声乐艺术的希望。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

结果:酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间,给药剂量为80μg/kg/d时疗效最显著,达到69.24%,在20μg/kg/d-80μg/kg/d剂量范围内生命延长率为50-70%,给药剂量与荷瘤鼠生存时间呈现一定量效关系;酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长,给药剂量为160μg/kg/d时疗效最显著,抑制率为44.03%,并且在40-320μg/kg/d剂量范围内抑制率为25-50%,给药剂量与肿瘤抑制率呈现一定量效关系;酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用,在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显,其中浓度为100μg/ml时抑制率达19.36%;酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤:体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强,与效应细胞对照组相比有显著性差异(P<0.05)杀伤率分别达到35.58%、61.2%;体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强,与生理盐水对照组相比有显著性差异(P 。05),杀伤率分别达到21.39%、47.63%;裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强,与生理盐水对照组相比有显著性差异(P.05),最高杀伤率分别达到32.86%、73.07%;酪丝亮肤能增强单核巨噬细胞系统的吞噬功能,吞噬指数与生理盐水组比较有显著性差异(P.05);酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO,与效应细胞对照组相比有显著性差异(P.05);酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO,与生理盐水对照组相比有显著性差异(P.05);酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO,IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰,酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能,而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。

Kinds of culture in 21st century are becoming compatible and fusional. Vocal art is of no exception. Based on the current situation andtrend, this article applies methods such as Historical-Descriptive,culture perspective and theoretical analysis to the research of singing skills of bel canto and the folk singing in order to analyze the art characteristics and the real possibility of fusion between them. At the same time, successful examples are combined to work out the possible way for the application of bel canto in folk singing and nationalization of bel canto.

二十一世纪的文化是兼容并蓄、融合发展的文化,二十一世纪的声乐艺术亦如此,正是基于这一现状和趋势,本文旨在运用历史描述、文化比较和理论分析等方法,通过对美声唱法和民族唱法歌唱技巧的研究,分析两种唱法的艺术特性和两者融通的现实可能性,同时结合一些成功的实例试图给出民族唱法美声化和美声唱法民族化的可能途径。

This paper analyzes the present situation of Bel Canto in our country, explores and ponders over the problems of the scientific nature, nationality and contemporaneity of Bel Canto, and advances the author's own understanding.

分析了美声唱法在我国的现状,对美声唱法的科学性、民族化、时代性等方面存在的问题进行了探索与思考,提出了自己的认识。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法,人肝癌细胞株Bel-7402与雷公藤甲素(10μg/mL、5μg/mL及0.25μg/mL)共培养,MTT法检测其对Bel-7402细胞增殖的影响,TUNEL技术检测其对Bel-7402细胞凋亡的影响,免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

G/ml. After wt-p53 transfection and VCR treatment, the number of Bel-7402 cells was decreased; the cells were swollen severely with irregular projections; pyknosis, karyorrhexis and karyolysis were observed. After wt-p53 transfection, the expression of mdr1 and P-gp in Bel-7402 cells was down-regulated remarkably, and the expression of TopoⅡα was up-regulated.

形态学检查转染细胞,在VCR作用下细胞数明显减少,细胞散在不成片、细胞高度水肿、胞体不规则突起,可见核固缩、核裂解、核溶解。p53基因对Bel-7402细胞的mdr1/P-gp的表达有明显的抑制作用,对TopⅡα基因的表达有上调作用,对GSTπ、MRP基因表达没有影响。

According to the characteristics of the bel canto and the regulation of the Chinese characters pronunciation,discusses phonation and vomit word of the bel canto at the time of singing the Chinese work.

根据美声唱法的特点和汉字发音的规律,论述了美声唱法在演唱中国作品时的发声与吐字问题。

In 1990, Will Smith was cast in the NBC sitcom, The Fresh Prince of Bel-Air, which starred Smith as the "Fresh Prince," a young street-smart kid from Philly living in one of LA's wealthiest areas, Bel-Air.

他是生活在费城的一个富人区——贝莱尔的一个年轻而又有小聪明的孩子。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响,ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量,检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

To investigate the interaction between heat shock protein 70(HSP70) and alpha-fetoprotein in human hepatocellular carcinoma cell line BEL-7402,the expression and localization of HSPT0 and AFP in human hepatocellular carcinoma cell line BEL-7402 were determined by immunocytochemistry and indirected immunofluorescence cytochemicai staining.

甲胎蛋白是由胚胎肝细胞、卵黄囊和胚胎消化道微量合成的主要血清蛋白[1]。

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We got alerted a couple of times while we were down south that HETs were on the way to bring us back up north because things were going to go hot again, but it was just rumors.

南下的途中我们不只一次得到警告说重型装备运输车将拉着我们重新北上,因为局势正在变得重新紧张起来,但这只是谣传。

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雄蕊6,着生的在花被基部筒部,内藏;花丝短;花药基着子房球状卵球形,3室;胚珠数个每室。