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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

To begin shaping your nails, start by trimming them with a quality clipper.

开始塑造你的指甲，开始微调他们提供一个高质量的帆船。

Architect Roj then proceeded to demonstrate how Progetto successfully put into practice these principles with a series of examples, most notably the Garibaldi Towers, located in Milan, that have been awarded the Gold LEED certification, and the Cobra Automotive Headquarters and Production Plant, located in Beijing, for which construction work is scheduled to begin next month and which also aims to obtain a Gold LEED Certificate.

罗伊先生还通过一系列的项目案例展示了波捷特公司如何将这些原则付诸于实践，最特别的就是座落在米兰的加里波第塔，已经获得了金质LEED绿色认证，以及位于北京的科博莱汽车总部及研发基地，该项目建筑工作将于下月开始，也将努力获得金质LEED绿色证书。

Our style is to begin each line in the comment with an asterisk, thus indicating that the entire range is part of a multi-line comment.

我们的风格是在注释的每一行以星号开始，指明整个范围是多行注释的一部分。

To add a comment, begin a line with an exclamation mark.

要增加注解，以一个惊叹号开始一列。

Now we begin to draw a circle with compasses.

现在我们开始用圆规画一个圆。

I knew, I should search a new cake. 8 years to China it is a great Olympic Games year, to Internet, I consider the year that also is an advance rapidly, everybody thinks the website oneself buys a concern with the Olympic Games wildly approximately, of course my steel tube alliance is not exceptional also, the website that I also think to let me is in Olympic Games year have a new breakthrough, I begin to make news of a few Olympic Gameses, add picture of a few Olympic Gameses.

我知道了，我应该寻找一个新的蛋糕了。08年对于中国来说是一个伟大的奥运年，对于互联网，我想也是一个突飞猛进的年头，大家近似疯狂的想把自己的网站跟"奥运"扯上关系，当然我的钢管联盟也不例外，我也想让我的网站在"奥运年"有一个新的突破，我开始做一些奥运新闻，加一些奥运图片。2个多月了，我发现我做的这些事情没有带来我想要得结果。

However , it is wise to conjoin your paid traffic efforts with organic efforts that way you can begin building those relationships and other alternatives to traffic .

不过，这是明智的 conjoin 您的付费交通的努力与有机的努力，这样，您就可以开始建设，这些关系和其他替代交通。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新