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Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

As we shall learn from the materials gathered and presented below, Vijaykumar was a mid-level administrator in a Krishnamurti school in India; he began to have some spiritual experiences and then, more problematically, began to have inflated ambitions to become a leading guru-figure himself..

我们将从下面收集的材料中了解到， Vijaykumar 从前在印度的一所克里希那穆提学校做中层行政人员；他开始有了一些灵性经验，然后，比较有问题的是，他开始有了要成为一个领军的大师级人物的膨胀的野心。

It began to transform into the active continental marginin the Late Proterozoic and by the Early Paleozoic, it began to be subducted andslipped sinistrally.

海西末期华北－西伯利亚两大板块已缝合，进入超碰撞阶段，区内花岗岩大量侵位并形成了一系列推覆构造。

Metaphysics popular during this period, Chinese scholars advocating elegant Tetrapanax, paintings and other works on the history of painting began to appear, landscape painting, flower began to sprout, this period focus on the mental state of the art of characterization and the temperament of the performance to the theme of literature, painting an increasingly popular .

这一时期玄学流行，文人崇尚飘逸通脱，画史画论等著作开始出现，山水画、花鸟画开始萌芽，这个时期的绘画注重精神状态的刻画及气质的表现，以文学为题材的绘画日趋流行。

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果：hMSCs诱导前为纺锤形，诱导后第2天部分细胞即开始发生形变，呈球形或短棒状，1周后胞浆中颗粒增多，约20～30%细胞边缘呈毛刷样变化；hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义，CD90未诱导时表达呈弱阳性，诱导后明显增高(P.05)；ANP和CX43在诱导前无表达，诱导后第2周开始表达且表达随时间逐渐增强，但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加，低浓度诱导组低于标准浓度诱导组，组间差异有统计学意义(P.05)，G_0/G_1期细胞比例诱导后较对照组显著减少(P.05)；β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强，在第6周时均达到高峰，第8周时表达开始衰减，Bcl-2、Bax基因表达呈时间依赖性变化，hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

I not only thought these the pure Productions of Providence for my Support, but not doubting, but that there was more in the Place, I went all over that Part of the Island, where I had been before, peering in every Corner, and under every Rock, to see for more of it, but I could not find any; at last it occur'd to my Thoughts, that I had shook a Bag of Chickens Meat out in that Place, and then the Wonder began to cease; and I must confess, my religious Thankfulness to God's Providence began to abate too upon the Discovering that all this was nothing but what was common; tho' I ought to have been as thankful for so strange and unforseen Providence, as if it had been miraculous; for it was really the Work of Providence as to me, that should order or appoint, that 10 or 12 Grains of Corn should remain unspoil'd,(when the Rats had destroy'd all the rest,) as if it had been dropt from Heaven; as also, that I should throw it out in that particular Place, where it being in the Shade of a high Rock, it sprang up immediately; whereas, if I had thrown it anywhere else, at that Time, it had been burnt up and destroy'd.

当时，我不仅认为这些谷类都是老天为了让我活命而赐给我的，并且还相信岛上其他地方一定还有。于是，我在岛上搜遍了我曾经到过的地方，每个角落，每块岩石边我都查看了一遍，想找到麦穗和稻秆，可是，再也找不到了。最后，我终于想起，我曾经有一只放鸡饲料的袋子，我把里面剩下的谷壳抖到了岩壁下。这一想，我惊异的心情一扫而光。老实说，我认为这一切都是极其平常的事，所以我对上帝的感恩之情也随之减退了。然而，对发生这样的奇迹，对意料之外的天意，我还是应该感恩戴德的。老鼠吃掉了绝大部分谷粒，而仅存的十几颗竟然没有坏掉，仿佛从天上掉下来似的，发生这样的奇迹难道不是天意又是什么呢？再说，我把这十几颗谷粒不扔在其他地方，恰恰扔在岩壁下，因而遮住了太阳，使其很快长了出来；如果丢在别处，肯定早就给太阳晒死了，这难道不是天意吗？

I started learning how to change habits a few years ago. Since then I've s witched to a vegan diet, began exercising every day, started writing new articles every day, began waking up earlier and trying some wacky experiments to improve my life.

我从几年前开始改变我的习惯，现在我已经养成以素食为主的用餐、每天锻炼身体、每天写新文章、早起等等好习惯，并且还不断尝试着新习惯来进一步提高我的生活。

Since then, he gave up on the studying for a while and entering 'Hapdong Film Company' in order to have experiences with dream to be an actor, he began as the junior staff going through planning department, directing department, and production department, etc., And then, challanging after seeing the public notice on the audition for KBS drama 'Salut d'Amour' directed by Yoon Seokho PD in 1994, as he was cast as the main character, he began to walk a path of full-scale actor.

从此之后，他有一阵子放弃升学，为了要体验演员梦而进入了& Hapdong电影公司&，他以资深工作人员的身份开始，待过企划部、导演部、制作部等等。后来，1994年尹锡瑚执导的KBS连续剧&爱的问候&公开试镜，裴勇俊挑战试镜成为主角之后，他就开始了全方位演员之路。

The remarkable idea that our universe simply began from nothing has not always been accepted with the conviction it is today and, from fiercely disputed leftfield beginnings, took the best part of the 20th century to emerge as the triumphant explanation of how the universe began.

&宇宙生于一无所有&这一伟大猜想直到今天还没被普遍承认（有极具争议的左派开端的原因），它逐渐成功解释了宇宙的开端使它成为二十世纪的骄傲。

The remarkable idea that our universe simply began from nothing has not always been accepted with the conviction it is today and, from fiercely disputed leftfield beginnings, took the best part of the 20th century to emerge as the triumphant explanation of how the universe began.

&宇宙生於一无所有&这一伟大猜想直到今天还没被普遍承认（有极具争议的左派开端的原因），它逐渐成功解释了宇宙的开端使它成为二十世纪的骄傲。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失