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The thesis consists of six chapters. In the first, the technologies of atomic force microscopy and of the measurement elasticity of biomolecules were introduced. In the second, the validity of VSPFM was confirmed by lift mode atomic force microscopy. In this chapter, the height of DNA was measured by lift mode atomic force microscopy, which demonstrated that the method of height measurement of biomolecules by VSPFM was correct and established the foundation of the method of measurement elasticity of biomolecules by vibrating mode scanning polarization force microscopy. In the third chapter, detailed work has been illustrated on the foundation of the method of measurement elasticity of biomolecules by VSPFM. And the compressive elasticity of DNA was measured. In fourth and fifth chapters, the method was applied in the measurement elasticity of proteins. Two proteins elasticity, fibre-like protein α-synuclein and global protein IgG, were measured by VSPFM, through which the method wound its way to the application of biomolecules. In last chapter, the final part of the thesis was a summary. A conclusion of the thesis and a self-comment on my work as a PhD candidate have been made, and expectation about the further works has been addressed.

本论文共分为六章，第一章，引言部分主要介绍了原子力显微镜技术及生物大分子弹性测量技术；第二章主要是VSPFM方法的正确性论证，介绍抬高模式原理，利用抬高模式原子力显微镜对DNA的高度进行测量，论证振动模式扫描极化力显微镜测量生物大分子的高度的正确性以及准确性，从而为振动模式扫描极化力显微镜测量生物大分子的弹性方法的建立奠定基础；第三章以脱氧核糖核酸为例详细介绍了振动模式极化力显微镜测量生物大分子弹性的方法的建立，对DNA的压弹性进行了初步的测量和分析；第四章和第五章介绍了振动模式扫描极化力显微镜在蛋白质弹性测量中的应用：α-synuclein和IgG分别是纤维状蛋白和球状颗粒蛋白，通过振动模式扫描极化力显微镜测量这两种蛋白质的弹性，摸索振动模式扫描极化力显微镜在蛋白质弹性测量中的应用；第六章对全文进行了总结，在对论文的工作进行归纳和自我评价之后，还对进一步的工作进行了展望。

In chapter three, the trait and composing of self-study ability have been analysed in brief, and the report of inquisition has been carried through about actuality of students self-culture, whose significance has been set forth in the last.

第三章简要地分析了自学能力的构成和特点，并对大学普物教学中学生自学能力的现状进行了调查和分析，最后阐述了培养学生自学能力的意义。

The folk culture ,as the part of the traditional culture has been payed more attention. The folk fine arts ,as the folk cultural important components, have been mentioned more .Therefore, the shadowgraph has also been included in the national non-material inheritance protection name list.

作为传统文化中一部分的民间文化被热烈的关注着，民间美术是民间文化中的重要组成部分，对于民间美术的整体关注度也越来越高，于是，皮影被列入了国家非物质遗产保护的名单中。

The domestic applying enterprises have been in good management conditions, their management of costs and quality has been strict, and there hasn't been any sign of bad operation or management.

国内申请企业的管理状况良好，成本、质量管理严格，没有经营管理不善的迹象。

The simple random sampling plan and stratified random sampling plan has been introduced and the application means and effects of which also has been presented,it has some characteristics such as material precision and obvious reduce of sample size,and rational advice has been presented.

介绍了简单随机抽样和分层随机抽样方法及其在检测中的应用方法和效果。

The paper aims to find out the motivation of franchisees for choosing the convenient store franchise chain. Based on the review of relevant literature, the possible motivation has been educed, and theoretical support has been found. Also, a simple random sampling method to questionnaire survey has been adopted.

在文献探讨的基础上推导出加盟商可能的加盟动机，并从已有的连锁体系形成理论中寻找相应的理论支持，并针对广泛采用连锁加盟的新型零售业态，连锁特许加盟便利店进行实证研究。

Hence, in this dissertation, the electrochemical measurement methods have been studied and a simple system for electrochemical impedance spectrum has been designed and the general program of electrochemical measurement software has been written.

因此，本文对微机化电化学测试方法进行了研究，设计了化学阻抗谱测试系统，编写了电化学测试软件的通用程序。

A slightness pipe inner surface TiN coating system has been designed and equipped, with this system, coating experiment on the inner surface of 2000mm long 86mm diameter stainless steel pipe had been done with well experiment results, with these experiments, a technique of TiN coating has been achieved.

和国际先进水平相比，采用辅助真空室设计方法使镀膜装置极为简单。

And so the Snowdrop was put back into the book, and felt equally honored and pleased to know that it was a token in the glorious book of songs, and that he who was the first to sing and to write had been also a snowdrop, had been a summer gauk, and had been looked upon in the winter-time as a fool.

这朵"夏日痴"于是便又被放到书里去了。它感到很荣幸和愉快。因为它知道，它是一本美丽的诗集里的一个书签，而当初歌唱和写出这些诗的人也是一个"夏日痴"，一个在冬天里被愚弄的人。

In study of SOFM, from basical SOFM structure and algorithm, the relationship between neighborhood function and sequential maps has been first analysed by defining the energy function, then learning converge of the network has been discussed and it has been proved that for the SOFM there is a reliable solution to presenting feature vector distribution.

对自组织特征映射的研究，从基本的网络结构和算法入手，首先通过定义能量函数分析了邻域函数与网络有序映射的关系，进而分析了网络的学习收敛性。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。