查询词典 beat into sb.'s head

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Hey yo jim man why don't ya, you don't you kick some of that you know you know how you do it man it's a trip people don't even believe were together right now but tell your story you know the one i like say it for me riders on the storm riders on the storm into this house we're born (into this house we're born) into this world we're thrown (into this world we're thrown) like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah goin' off of this off of that with the lizard king bumpin' in the back how bout that driftin', liftin', swiftin', coastin', testaroastin' but the wheels won't stop 200 on the highway fresh up off the block he's a rider, na he's a killer dresses in all black but his hat says stealla pedal to the metal i gotta go hard drive by and say hello hey fredwreck you my mello now let me here what i sound like acapella wow ride dip swish now bring it back just like this like a dog with out his bone unlike a g with out his chrome it's hard to imagine the homey dog in a jag and he's checkin' for the checkered flag comin' in first never in last cause my car to fast i neva eva run out of gas cause i just to clean i do it upper class so fasten your seat belts its so hot it will even make heat melt so get a bowl and roll and ride slip through the slip and slide like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah need for speed i'm trying to take the lead hold on little homey before you run into the trees i've seen thinks that i would have never saw before hey yo jim let'em in, let'em in open up my back tire smokin' the whole street and now the police wanna flash there lights and chase the dogg all night but i won't pull over nor give up cause i just don't give a fuck yeah from the side boy where we was born and raised straight up to ride boy continuously, we get to an expeditiously keep the light on east side on snoop dogg and the doors and yeah we bout to ride on riders on the storm riders on the storm into this house we're born into this world we're thrown were thrown

嘿摇摇弯轨机人为什么不唉，你不你踢一些那你知道你知道如何你这样做人这是一次旅行人们甚至不相信是在一起此时但告诉你的故事你知道那个我像为我说它骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔像个狗没有一根骨头一个演员那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀去'离开对于这离开关于那和蜥蜴国王 bumpin'在后面如何拳击比赛那 driftin'，liftin'，swiftin'，coastin'，testaroastin'但是这个车轮不会停止两百在公路上新鲜向上远离块他的一个骑士，娜他的一个杀手衣服在纯黑但他的帽子说stealla 踩踏板金属我有去难驶过和问好嘿fredwreck 你我的梅洛现在让我这里什么我听起来像acapella 哇骑现在倾斜豪华带来它回来只是这样像个狗与出来像个狗没有一根骨头一演员借出的骑士在暴风雨上那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀需要速度我是试图着先鞭继续小像家一样在你未碰撞树木我已经看到想那我从来不锯在以前嘿摇摇弯轨机让'em 在，让'em 在开发我的后面疲劳smokin'一街一巷那么警察要闪光那儿灯光和追逐道格整夜但我不会拦截也不放弃造成我只是不给一个性交是呀自边男孩我们在那里出生和提升直直至骑男孩骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔我的翻译不太标准别见怪

The locking mechanism is configured to permit selective angular positioning of the head relative to the handle on the pivot axis and is operable between an adjusting mode in which the head is angularly positionable relative to the handle and a locking mode in which the head is locked in an angular orientation relative to the handle.

锁定机构的构形做成容许选择在枢轴线上头相对于柄的角度定位，并且可在头可相对于柄改变角度位置的调节模式和头锁定在相对于柄的角度位置上的锁定模式之间工作。

Then picked up electric razors, Banzhu sheep''s head start with the neck, and then to the abdomen, and then around to the back of the body, steering head, within a few minutes, you can cut down the entire wool, as if lifting a sheep under the same , shearing off the wool from ranchers by category, screening, head and other parts of the wool is short, thick, dirty, are beneath contempt, back, abdomen, fine wool, soft, long part of high-grade

之后操起电动剃刀，扳住羊头先从颈部，再到腹部，之后绕到违脊，转向头顶，几分钟之内，就可以把整张羊毛剪下来，就好象掀下一张羊皮一样，剪下来的羊毛由牧地主分门别类筛选，头上等部分的羊毛短，粗，脏，属于下品，违部腹部的羊毛细、软、长属于高档

As the hips are usually smaller than the head, so may be relatively rapid decline, but not for the head is also very easy to output, and consider it premature to production timing, so as to avoid birth trauma or head down in the final crisis of Health, If there is any way to extend the middle class must be an early Caesarean section.

然而，在目前的环境下，个人认为第二胎以上，没有剖腹产的孕妇，在胎儿不大，孕妇本身也没有并发症的情形下，臀位也就是"纯臀位"没有直立腿的情形，在产程正常时，还是可以考虑阴道生产，不过事前与主治医师讨论利弊后再下决定，是最佳之道。

On the basis of the research on shape of fish-head boudin and the difference between layer and matrix, and by means of the method of physical modeling, the author modeled different shapes of fish-head boudin, and came out the relationship between shape of fish-head boudin and viscosity ratio between layer and matrix, which can be called an experience rheological gauge.

在深入研究鱼嘴构造形态与能干层基质流变性差异的基础上，运用物理模拟的方法，模拟出不同形态的鱼嘴香肠构造，反演出能干层基质粘度比与香肠形态之间的关系，进而得出一种关於鱼嘴香肠构造的经验流变计。

This invention discloses a multi-queue sequence buffer management circuit and a method based on a pipeline applying a pipeline structure including: an arbitration circuit selecting one for process from read, write and distribution buffer requests, a buffer slot state module designing state of the slot requiring operation and queue numbers and assigning idle slots, a buffer slot filter module filtering the slot, a buffer slot filter module filtering the slot states not belonging to the current operation queues nor idle aligned in terms of the head pointer, a queue slot selection module computing continuous idle slot numbers from the slot pointed by the head pointer and refreshing the head pointer and selecting preparing slots, a queue slot prior queuing module refreshing the read pointer and result numbers of the current operation queues with the pointer of the first prepare slots and their numbers which can support multi-queue to share one buffer space, queues can access the buffer in overlap.

本发明公开一种基于流水线的多队列顺序化缓冲管理电路及方法，本发明电路采用流水线结构，包括：仲裁电路，从读、写、分配缓冲请求中选取一路进行处理；缓冲槽口状态设置模块，设置请求操作的槽口状态和队列号，分配空闲槽口；缓冲槽口滤除模块，滤除不属于当前操作队列且非空闲态的槽口状态，按头指针对齐；队列槽口选择模块，计算头指针指向槽口起的连续空闲槽口数并更新头指针，选出预备态的槽口；队列槽口优先排队模块，用第一个预备态槽口的指针和预备态槽口数分别更新当前操作队列的读指针和结果数；本发明可以支持多个队列共享一个缓冲空间，各类指令队列能对缓冲进行交叉访问，并对指令结果的写入读出进行顺序化管理。

An aerosol spray device which is capable of imparting a unipolar charge by double layer charging and charge separation to liquid droplets of a composition sprayed therefrom has a spraying head in the form of an insert in an actuator, the spraying head having a bore through which liquid is expelled having an outlet, preferably with a tortuous periphery, having an L/a ratio of at least 8 (preferably at least 10) where L is the length of the periphery defining the bore outlet in mm and a is the cross-sectional area of the bore outlet in mm2 and the apparatus being constructed such that the droplets are expelled from the spraying head at a flow ratio of at least 0.4 (preferably at least 0.5) grams per second and have a charge to mass ratio of at least +/-1x10-4 C/kg.

本发明还提供了一种喷射装置，它能够通过双层充电和电荷分离而向要喷出的组合物液滴施加单一极性的电荷，该喷射装置具有一个喷头，该喷头制成为致动器中的插入件的形式，喷头具有一个孔腔，液体能够通过该孔腔从装置中排出，该孔腔具有一出口，该出口最好具有曲线周边，并且L/a比率至少为8，其中L是以mm为单位的确定孔腔出口的周长，a是以mm2为单位的孔腔出口的横截面积，该装置构造成使得液滴在至少每秒0.5克的流动速率以及具有至少＋／－1×100C/kg的电荷／质量比的状态下从喷头排出。

After washing a head every time, damp head is clammily on the head really afflictive.

每次洗完头后，湿漉漉的头发粘在头上真难受。

Among them, in tumour of glue mother cell, visible head pledges fasciculus is interrupted in vain, its stop fiber to be able to be through corpus callosum; in meningeal tumour, visible tumour oppresses the head that cause pledges the fiber shift and remaining, fasciculus; that is departed is in in vain a tumour of cell of metamorphic star form is medium, DTT expression sends place head to pledge fasciculus is interrupted in vain formerly for tumor and tumor countershaft dash forward infiltration.

其中，在胶母细胞瘤中，可见脑白质纤维束中断，其终止纤维可通过胼胝体；在脑膜瘤中，可见肿瘤压迫引起的脑白质纤维移位以及残存、被分离的纤维束；在间变性星形细胞瘤中，DTT表现为肿瘤原发部位脑白质纤维束中断以及肿瘤对轴突的浸润。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。