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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

Section in situ hybridization demonstrated that the Fstl1 transcripts in cervico-lumbar spinal cord were distributed in the gray matter neuro-cells and ependymal cells. hybridization signals were present widely in dedifferentiated cells and mesenchyme cells at 2 wks and 3 wks while also in cartilaginous cells at 3 wks of regenerating blastema after tail amputation.

原位杂交结果显示，多疣壁虎脊髓中Fstl1的阳性信号主要存在于灰质神经元细胞及室管膜细胞；而在断尾再生2~3周尾芽中，Fstl1阳性信号广泛存在于再生尾芽的新生区未分化细胞及间充质细胞，3周尾芽中，阳性信号还存在于软骨生成区。

Was presented in 12 cases (24%) by bacterial isolation. By in-situ hybridization, PCV2 signals were chiefly distributed in interstitial and necrotic lesions, and the positive signals could be found in macrophages-like cells and necrotic debris. PRRSV signals were mainly located at interstitial lesions and alveolar wall, and the positive signals could be found in macrophages-like cells and epithelial cells of alveoli. Swine influenza virus could be found in interstitial and necrotizing lesions, and the signals could be found in macrophages-like cells, and epithelial cells of terminal bronchiole and alveoli.

另运用原位杂交法对PCV2、PRRSV及猪流行性感冒病毒进行检测，结果发现PCV2主要分布於PNP的间质及坏死病灶区，并能於肺泡及终末细支气管上皮细胞、巨噬细胞与坏死细胞碎片皆可见有病毒核酸；PRRSV则多分布於间质病灶区及肺泡壁处，能於巨噬细胞与肺泡上皮细胞见到病毒核酸；SIV则存在於间质及坏死病灶区，且能於肺泡及终末细支气管上皮细胞与巨噬细胞见有病毒核酸。

Results (1)The agger cells existed in 99% of the cases, the development of the agger cells in 9～16 years old children, and adult had no difference;(2) The morphology of agger cells differed widely, but its relationship to the anterior border of the middle nasal concha, bulla ethmoidalis and nasolacrimal canal was quite stable;(3) The upward pneumatization of the agger cells was not related to deviation of nasal septum, but closely related to the development of the frontal sinus;(4) The anterior nasosinusitis often influenced the agger cells and might result in hyperosteogeny.

结果 ①绝大多数病例（99%）有鼻丘气房，儿童（9～16岁）鼻丘发育与成人相比无差异性；②鼻丘形态变化很大，但与中鼻甲前端、筛泡和鼻泪管的关系是恒定的；③鼻丘向上气化程度与额窦发育关系密切，鼻中隔偏曲对其发育无明显影响；④前组鼻窦的广泛炎症易波及鼻丘，并可出现鼻丘骨质增厚。

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果：1、白藜芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长，同时促进细胞的分化和凋亡：白藜芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化，UW228-3、Med-3细胞向神经元细胞方向成熟分化，以100μM作用48小时效果显著；2、四个细胞系均有Fas和Caspase-3表达，但无Fas-L基因表达和蛋白活性产生，因此难以形成Fas相关性死亡通路；3、白藜芦醇处理前后CYP1A1基因的表达在各细胞系略有不同：UW228-1，-2，-3三个细胞系白藜芦醇以剂量相关性方式上调CYP1A1的表达，而Med-3细胞系，白藜芦醇却抑制CYP1A1的表达；四个细胞系在白藜芦醇处理后CYP1B1的表达均受到抑制；4、UW228-3细胞系经白藜芦醇作用后细胞周期有较大改变，表现在G0/G1期在整个细胞周期中所占的比例增加，而其它时期则相应减少。

On December 30, 2005, Daystar completed shipments of its TerraFoil and TerraFoil-SP solar cells. Nearly 500, 10cm x 10cm TerraFoil cells were shipped to Titan Energy in India, the contract PV module manufacturer of DayStar's first commercial customer, Blitzstrom GmbH, in Germany. A second shipment of over 300 cells, including a variety of smaller size TerraFoil-SP solar cells, was provided to Micro Energy Group of Zuhai, China, DayStar's second commercial customer. These cells will be used to produce a series of unique PV module prototypes for use in MEG's expanding line of solar powered consumer products.

截止2005年12月30日，日星科技完成了"特瑞伏"和"特瑞伏-SP"的生产计划，包括为印度太阳神能源公司和德国的Blitzstrom GmbH公司制造的50010厘米X10厘米的特瑞伏电池，后者是日星的第一个签约商用客户；此外为中国珠海的微能源集团公司供应300单元的光伏电池，包括各种小尺寸的"特瑞伏-SP"，以适应该公司拓展各种用途的太阳能消费产品的需求

Exogenous E2 alone has no effect on cell viability and apoptosis of H9C2 cells, but it can decrease the sensitivity of H9C2 cells to H_2O_2,hence,protect cells against apoptosis caused by oxidative stress.2Exogenous E_2 has downregulation effect on TβRⅠand TβRⅡof H9c2 cells,blocking ER may delete this effect of estradiol.H9c2 cells express ERβonly,suggests that E_2 may exert modulating effect on TGF-βpathway through ERβ.

外源性E2本身并不能影响H9c2细胞凋亡，但能够降低H9c2细胞对H_2O_2的敏感性，保护细胞抵抗氧化应激引起的凋亡。2外源性E2对H9c2细胞的TβRⅠ以及TβRⅡ具有下调作用，阻断ER可消除E2对TβRⅠ和TβRⅡ的下调作用；E2可进一步通过TGF-β通路降低受体后磷酸化smad2/3的水平；H9c2细胞仅表达ERβ，提示E2可能是通过ERβ发挥其对TGF-β通路的调控作用。

At last, the classification method is discussed in accidence and a classification method based on decision-tree is presented. In the method, the characteristic parameters of cells are extracted through different methods such as the morphologic, chroma and texture of cells, and then normal cells are eliminated gradually by the means which screens out normal cells from roughly to accurately, and in the end suspicious tumor cells are sorted according to their dubiety.

最后，对细胞的分类方法进行了深入的研究，提出了一种基于判别树的层次分类方法，采用细胞的形态学、色度学和纹理等不同方法获取的细胞特征参数，采取"由粗到精、逐步筛选"的策略逐步剔除正常细胞，并根据可疑程度对疑似肿瘤细胞排序，辅助病理专家的诊断工作。

Xinjiang Daye'is higher than male sterile lines. 2、The pistil characters of the male sterile plants BC1-2、BC1-19、BC1-21、BC1-22、BC1-23 are improved. Hairy cells of stigma of the male sterile plants enwrap loose mastoid cells. The area of mastoid cells exterior is larger. Hairy cells of stigma of the male sterile plants are longer and abduction.Their Style is larger and wide. The cubage of the epidermal cell is larger and the arrangement of passage cells is tight.

BC1-2、BC1-19、BC1-21、BC1-22、BC1-23的雌蕊的性状得到改良，改良的杂交后代不育株的柱头毛状体细胞将中心的乳突细胞包裹较疏松，乳突细胞明显突起且裸露面积较大，毛状体细胞较原不育系MS-4的相对长而外展且分泌物较多，；改良的杂交后代不育株的花柱较原不育系的花柱宽且长，表皮细胞的体积较大，通道细胞排列较紧密。

Strain AR-15 penetrated the exodermal passage cells through the velamen cells or root hairs and further infected the cortical cells. There were a number of spores and hyphae in plant roots that was damaged by strain AR-15. Study on the ultrastructure of D. nobile root and its endomycorrhizal formationshowed that the wall of velamen cells of D. nobile root was thin, and there were waxylayers outside of velamen. Cells of exodermis were arranged tidily, having thick wallsand chloroplast.

金钗石斛根及其菌根的超微结构研究表明，金钗石斛根的根被细胞薄壁，在最外层根被细胞之外覆盖着蜡质层，外皮层细胞排列整齐，细胞壁厚，内含叶绿体，靠近外皮层的皮层细胞含叶绿体、线粒体，皮层细胞壁薄，细胞核明显，周围有叶绿体围绕，细胞膜附近有小泡结构。

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我们普遍有大祸降临的感觉。