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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明，乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当，并有逐年上升的趋向，已成为院内感染的重要条件致病菌[1]，对多种抗菌药物有较高耐药性[1，2]，为此，我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况，对14种抗菌药物的耐药性及耐药趋向，现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本，经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例，同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测，1998~2000年使用GNI鉴定卡，2001~2004使用GNI+卡。

The result indicated that: the amount of the excess HF formed direct ratio to the yield of phosphorus pentafluoride and the optimum amount of the excess HF used is about 35%; the trickling rate of oleum shouldnt make the temperature of hexafluorophosphoric acid exceed 25 ℃ and the amount of oleum is 110% to 125% that of stoichiometry so that the content of phosphorus pentafluoride can be achieved 90%; the time and temperature of heating hexafluorophosphoric acid influenced both the yield and the content of phosphorus pentafluoride, and the optimum time should be about 30 minutes and the proper temperature was 148 to 153℃; when alcohol used asrefrigerant, the content of phosphorus pentafluoride was 94.03%, and the content can be up to 97.38%, provided that using liquid nitrogen as refrigerant.

通过实验发现，氟化氢用量与五氟化磷的产率成正比，最佳过量百分率宜在35％左右；发烟硫酸的滴加速度不能使中间产物温度大于25℃，发烟硫酸用量大于理论量10％～25％时，得到的五氟化磷纯度高于90％；六氟磷酸的加热温度对五氟化磷的产率和纯度均有影响，宜在148～153℃的范围内，加热时间应在30分钟左右；用工业酒精做冷凝介质得到的五氟化磷纯度最高只能到达94.03％，使用液氮做冷凝介质可以使五氟化磷的纯度达97.38％以上。

Machining process and general information:· General Information – Tooling o Plan to have own engineering group as company expands o Hwasung is subcontracted to do engineering currently o Uses same cutting tools as in Korea facility – all diamond o Regrind tools if can o Hwasung has capability to surface grind, repair dies, and make molds with wire EDM process · General Information – Machining o Boundary samples are used: okay, borderline, not good o Quality room has separate windows for incoming and outgoing parts o New equipment will be coming in from FM LaGrange plant o Each operation has pictures and job instructions for what to check and which gage to use to check part o Parts are manually loaded into machine or pallet, depending on line o Auditor checks parts for verification: after operator checks part, puts in bin, auditor checks part and reloads back on line o Have tool management system o A pokeyoke check is used for orientation prior to loading o Machines are guarded o Mostly have CNC machines o Manual chip collection systems o If automated, have robots with grippers to load/unload parts from pallets o After final inspection, operator visually inspects and loads onto plastic dunnage with the crown up and loads into the washer o Repairs: filing down of burrs or sharp edges after anodizing the crown o Have 2 Excello machines for special machining of the pin bores o 2.7% scrap for gasoline pistons o Cycle time is 18 seconds for gasoline piston o Process – Wash o Spray system o 4 stations: heated wash, wash with degreaser, 2 rinse stations o Uses RO water o Operator blow dries piston and puts on dunnage with crown up o Parts wheeled into coating room o Needs major improvements here o Chemical analysis done for bath in chemical room o Comments/Observations: o Relatively small piston facility, but plans for additional space.

加工工艺和一般信息：·–的一般信息工具 o 有自己的工程集团，随着公司的扩展计划 o Hwasung 是分包做当前工程 o 用途相同的扦插工具，如在韩国设施–所有钻石 o 回收料工具如果可以 o Hwasung 已表面研磨，修复模具，和模具丝电火花加工能力过程·一般信息–加工 o 界限示例用于：好交界，不好 o 质量房间有单独的窗口传入和传出的部件 o 新设备会在来从调频拉格朗日植物 o Each 操作具有图片和工作说明的什么复选，计为使用检查部分 o 部件是手动加载到机器或托盘，取决于行 o 审计检查验证零件：置于 bin，运算符检查部分后，核数师检查部分并重新加载回行上 o 有工具管理系统 o 为 pokeyoke 复选用于在加载之前的定位 o 机被保护 o Mostly 有数控机床 o 手动芯片收集系统 o 自动，如果有与要加载/卸载部分从托盘 grippers 机器人 o 后最终检验，运算符将直观地检查并加载到与塑料衬垫冠了与到洗衣机负荷 o 修复：提交下毛刺或锋利的阳极氧化冠后 o 有 2 Excello 机特别加工的销孔 o 2.7%废料的汽油活塞 o 周期时间是为汽油活塞式 18 秒 o 过程–洗 o 喷射系统 o 4 站：加热洗、与脱脂剂洗、 2 冲洗站 o 使用反渗透水 o 运算符吹干活塞式并将了放冠衬垫 o 部件轮式进涂层房间 o 需要重大改善在这里 o 为在化学的房间里浴做的化学分析 o 注释/结论： o 相对小活塞式设施，但额外的空间的计划。

You dont miss your water till the well runs dry As I close my eyes, sit back while reminiscing Of when we used to fuss and fight but end up kissing There may be sad and painful times along the way But in my heart you'll always be everything and more to me For I know this love between us is growing stronger You can call me wheneer, from wherever just remember that I'll be there throught all the stormy weather Us break up never, no, we'll be together forever You dont miss your water till the well runs dry But i believe so strongly with you and i Can somebody answer me the question why?

你没有错过你的水到井枯竭在我结束我的眼睛，坐而回忆当我们用大做文章战斗，但最终接吻可能有悲伤和痛苦的时候沿途但在我心中，你永远都应该多给我我知道这爱情，我们之间日益强大你可以随时打电话给我，来自何处，只记得说反正我没有想到所有的风雨天气我们分手没有，没有，我们将永远在一起你没有错过你的水到井枯竭但我相信，如此强烈地与你和我有人可以回答我的问题，为什么？

Up till now, the main pump types used in CPB are roller pump and centrifugal pump, but these two kinds of pump both have their own disadvantages. When roller pump is used, the blood cells will be destroyed severely and when the pipeline is kinked, air will be transported into body or the conduit will be broken. The function of centrifugal pump is better than that of roller pump, but now, this kind of pumps are all designed for adults, and when the flow rate is low, the function of protecting the blood is not significant.

目前，滚轴泵和离心泵是心肺转流中的常用泵型，但是滚轴泵对血液有形成分的破坏较严重，而且有引起泵管的崩脱和大量气体泵入体内的危险性；离心泵虽然在性能上优于滚轴泵，但目前的离心泵大都是为成人设计的，而且在低流量时在血液破坏方面的优点不明显，此外它们还有一个共同的缺点就是预充量均大，因此两者都不适合在幼婴儿体外循环中应用。

The superstructure uses the standard drawing, the substructure has used the gravity T-shape abutment and the rectangular buttress, in the design carries on the size to the abutment and the bridge pier to draw up, simultaneously satisfies the design the principle, the abutment uses 9.3m, in satisfies the structure and under the operation requirements premise, the abutment uses 9.3m to be long, because the geological condition decided, the pillar body uses 12m to be high, the open excavation foundation, the process load computation and the load combination, has carried on the bridge abutment and the pillar bottom section stress separately to the abutment and the bridge pier examines calculated, examines calculated the result conforms to the standard requirement, the bridge span uses the straight line build, and established this bridge construction organization plan, including the engineering project characteristic, the construction plan, the job practice, the bridge building construction technology, the measure has drawn up the top cap and the tray structure and matches the muscle chart, finally has drawn up Shui Xigou the bridge overall and the horizontal plan.

上部结构采用标准图，下部结构采用了重力式T 形桥台和矩形桥墩，设计中对桥台和桥墩进行了尺寸拟定，在满足构造和使用要求的前提下，桥台采用9.3m长，由于地质条件决定，墩身采用12m高，明挖基础，经过荷载计算和荷载组合，对桥台和桥墩分别进行了台底和墩底截面应力的检算，检算结果符合规范要求，桥跨采用直线布设，并且编制了该桥的施工组织设计，其中有工程项目的特征、施工方案、施工方法、桥梁工程施工技术，措施绘制了顶帽及托盘构造及配筋图，最后绘制了水溪沟大桥的总体及平面图。

The superstructure uses the standard drawing, the substructure has used the gravity T-shape abutment and the rectangular buttress, in the design carries on the size to the abutment and the bridge pier to draw up, simultaneously satisfies the design the principle, the abutment uses 9.3m, in satisfies the structure and under the operation requirements premise, the abutment uses 9.3m to be long, because the geological condition decided, the pillar body uses 12m to be high, the open excavation foundation, the process load computation and the load combination, has carried on the bridge abutment and the pillar bottom section stress separately to the abutment and the bridge pier examines calculated, examines calculated the result conforms to the standard requirement, the bridge span uses the straight line build, and established this bridge construction organization plan, including the engineering project characteristic, the construction plan, the job practice, the bridge building construction technology, the measure has drawn up the top cap and the tray structure and matches the muscle chart, finally has drawn up Shui Xigou the bridge overall and the horizontal plan.

内昆铁路水溪沟大桥，中心里程为DK608+393.00，该桥的设计方案有三个，通过技术、经济比较，采用4×32m预应力混凝土简支梁桥方案，桥梁全长149.5m，I级单线铁路，设计时速为120km/h，设计荷载为中活载。上部结构采用标准图，下部结构采用了重力式T 形桥台和矩形桥墩，设计中对桥台和桥墩进行了尺寸拟定，在满足构造和使用要求的前提下，桥台采用9.3m长，由于地质条件决定，墩身采用12m高，明挖基础，经过荷载计算和荷载组合，对桥台和桥墩分别进行了台底和墩底截面应力的检算，检算结果符合规范要求，桥跨采用直线布设，并且编制了该桥的施工组织设计，其中有工程项目的特征、施工方案、施工方法、桥梁工程施工技术，措施绘制了顶帽及托盘构造及配筋图，最后绘制了水溪沟大桥的总体及平面图。

Star was the sword attack, flash, freezing quickly locked the other side, take off, this time to the general efforts to the feet, do not depression bound him, do not stop after immediately change the tree, if you caught the foot, you can celebrate, and Star was going to die, the sword, and so on up the tree to the end of a sleep, the time the foot把抓End mill, the trees and the stars in the sword of time sleeping, watching monster monster output decision is to run away or destroyed, if no other grasping feet bound CD case of skills, it is recommended先跑, his recovery is not possible to recover your death, if the other side of the grasping foot is also used to recover you, waiting for him, a few such as the attack was once again changed the scope of tree, distanced themselves from 15-20 Ma, delay + hell + fire cage fire burning, in general are run each other this time, do not run in general have been burned to death, after all, did not grasp the foot of the stars would like to catch a sword魔道is unlikely, if you go chasing it, chasing burning, ice is almost bound to be a good CD it, well used, like playing catch up with you.

被剑星偷袭，闪现，迅速锁定对方结冰，起飞，这个时候一般抓脚要来了，别忧郁束缚他，完也别停，马上变树，如果你被抓了脚，你可以庆祝了，这个剑星要死了，等树要结束了补个睡，把抓脚的时间磨完，在剑星树和睡的时间，看怪物血量决定是把怪物打掉还是跑开，如果对方没用抓脚，束缚技能CD的情况下，建议先跑，他追是不可能把你追死的，如果对方用过了抓脚还来追你，等着他，几如攻击范围就是再次变树，拉开距离15-20玛，延迟+地狱火+火笼灼烧，一般来说对方这个时候都是要跑，不跑的一般都被烧死，毕竟没有抓脚的剑星想抓到一个魔道，不太可能，跑的话你就追吧，追着烧着，结冰束缚也差不多要CD好了吧，好了就用，追上一下打一下。

Oh, perhaps to his own too much pressure on it, yes ah, the Shanghai is full of people looking forward to countless people and reverie of the city, on a subway will be able to feel like me who are trying to fight the Workers, used to get up very early (not hard, afraid to go to work late because of salary deduction), used to the unit within the Office of the breakfast (because of the way, all vehicles coming and going, the crowd rushed, there is no way you To free a hand to feed the stomach) used to join the crowded elevator, used to listen to the words do not know how to Shanghai ......

不敢想！唉，也许是自己给自己的压力太大了吧，是啊，上海这座让人充满无数憧憬并且让人遐想的城市，一上地铁就能感受到那些和我一样正在努力拼搏的打工者，习惯了很早起床（不是因为勤快，因为怕上班迟到扣薪水），习惯了在单位的办公室内早饭（因为路上、车上全是来来往往、急匆匆的人流，根本没有办法让你腾出一只手来喂饱肚子）习惯了一起挤电梯、习惯了听不大懂得上海话。。。。。。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。