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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

You'll have to know how to scuba dive to reach your room, and guests without the mandatory certification must take a course at the hotel. Once you've reached the lodge, which sleeps up to six, you'll be close to angelfish, anemones, barracuda, oysters, and other creatures — each room is equipped with a 42-inch window, so you don't need to be suited up to keep an eye on the neighborhood.

你必须懂得如何潜水抵达你的房间，客人们没有义务保证必须按一定的航道到达酒店，一旦你抵达酒店就可以睡到六点钟，你可以接近扁鲨、海葵、梭鱼、牡砺和其他生物——每间房都装备了42英寸视窗，所以你没有必要穿上衣服留意你的邻居。

I didn't want to be bound up in any roadblock or be bound up with her, because I knew my boss would be bound to realize that I was not at work if this event was on the TV news.

我不想热中于任何道路封锁，也不想与她有什么密切关系，因为我知道如果这事上了电视新闻，我的老板一定会发现我没有上班。

I didn't want to be bound up in any roadblock or be bo und up with her, because I knew my boss would be bound to r ealize that I was not at work if this event was on the TV n ews.

我不想热中于任何道路封锁，也不想与她有什么密切关系，因为我知道如果这事上了电视新闻，我的老板一定会发现我没有上班。

Day, he one when hunting in the forest,he caughtsight of artemis'' fair maidens,the seven pleiades .hewas so attracted by their beauty that he started chasing them.indespair the charming maidens prayed to artemis for help and were sent up by the goddess to the heavens as a constellation .later he fell in love with another maiden,merope by name,butrash and impatient,he took the maiden by force and enraged her father,king oenopion of chios.the king made him drunk and put out his eyes .following the sound of the cyclopes'' hammer,he went to hephaestus,who sent a guide to travel with him to the east,where the sun rose.exposing his eyeballs to the rays of the sun,he was restored to his eyesight.he became aconstant companion of artemis and followed the goddess in the chase with his dog sirius.apollo did not approve of this love, however.one day he dared his sister to shoot at a dark spot moving in the sea.little did she suspect that she was aiming herarrow at orion,for the dark spot turned out to be the head of orion swimming in the ocean.artemis was extremely sad.as she could not pull him back from the grasp of death she sent him up to the sky as a constellation,with his hunting dog sirius be sidehim.

他是海神波塞冬漂亮的儿子。一天，他在森林中打猎，看到了阿尔特弥斯的仙女们——七颗明星。他如此爱慕她们的美丽，便追赶她们。困境中，美貌的仙女们向阿尔特弥斯求救。结果女神将她们都送入天空充当星座。后来奥利安爱上了另一少女墨洛珀。他鲁莽急躁地施暴于她。这激怒了少女的父亲俄诺皮耳国王。国王把他灌醉后，挖出了他的眼睛。随着"独眼巨人"大锤的敲响，他来到火神赫斐斯托斯跟前。火神派向导带他去太阳升起的东方。将眼球暴露于阳光后，他就恢复了视力，成了女神阿尔特弥斯永久的伴侣，并带着他的猎狗天狼跟随女神打猎。阿波罗并不赞成他们之间这种恋情。一天，阿波罗怂恿阿尔特弥斯向海中一移动的黑点射箭。她根本没想到自己是将箭瞄准奥利安。当看清黑点竟是在游泳的恋人奥利安的头时，阿尔特弥斯悲痛万分。因她无力使他起死回生，只好将他送入天空作一星座。他的猎狗天狼则蹲在他的身旁。

Institutional harmonize mechanism should be build up. Economics input and output calculation system ought to be applying to the mechanism. The benefit-guiding mechanism of family planning policy should be established in floating population as soon as possible, to set up the benefit-guiding mechanism of family planning policy dominant with endowment insurance project, and calculate exactly the quantity of the including people and finance, and ensure its function. This paper has some referenced effect.

针对这些存在的问题，作者提出了相应的政策建议，包括：确立计划生育利益导向给付制度在社会保障体系中的法律地位；建立更加制度化的以计划生育部门为主的协调运作机制；建立计划生育利益导向投入与产出的经济核算体系；建立针对流动人口的利益导向机制；逐步建立计划生育养老保障与医疗保障为主导的利益导向机制，突出养老与医疗保障的作用；准确测算目标人群的数量与今后资金的需求，加大保障力度。

An introduction is a must I bust these rhymes So now the time for this MC to go for mine All tied up with your girl is naughty Check it, change to an ultimate groove, and this DJ's got flow for you Flows in twos in fours straight rap combos Straight bumbin in my ride layed back eyes closed, Crusin through the sand-cadillac man I'm about to tell you what is the game plan Move from the valley up to the higher land Rock groove Seoul Bangkok and Japan Sippin Ginseng brew bro, I thought I told you Your girl was colockin the kid so he had to Ah, ah I want to drive like you I want to be down with your Cadillac crew My man's in the Eldorado Cadillac Picking up sisters milky like similak You best believe he got the shinny hub caps You best believe he got the gold teeth caps My man's in the fleetwood and it's all good Picking up actresses placing them on mattresses, Rolling over cactuses Running things like laxatives Cadilliac spedialist, the most spectacalist Southwest Cadillac rollin crew Kicking back as we bust these rhymws for you, low ridin in a Cadillac is a must, As we bimpin'straight beat from the back of the trunk

概论是我一定胸像这些儿歌所以现在时间是mc去矿井一切捆绑你的女孩是顽皮的检查，转变为一个最终槽， d这个dj的got流为你流twos会合直rapcombos直bumbin我坐la 李明回闭眼 crusin通过砂凯迪拉克男子，我要告诉你的是什么游戏规则，从河谷到上级土地岩坡汉城曼谷和日本sippin人参brew买，我笔休我告诉你一女童被colockin流星语于是只好啊，啊我要驾驶你一样，我想我们应该让你的卡迪拉克船员男子汉的在eld orado凯迪拉克拾乳熟姐妹一样similak你最好相信他抓到非煤枢纽头套，你最好相信他有金牙头套男子汉的在fleetwood和它的一切好拾演技使它们床垫，滚动cactuses办事情泻剂cadilliacspedialist，最spectacalist西南凯迪拉克rollin队员踢回，因为我们这些胸像rhymws你低 ridin在凯迪拉克是必须的，因为我们bimpin'straight击败来自背面的主干

The advantage of Servicemix: 1, be based on JBI standard; 2, can heat up deploy; 3, supportive Camel (can develop compositive technological process with DSL); The defect of Servicemix: 1, JBI standard was brought use those who go up is trival, and JBI standard did not gain too much favour, the future was not predicted; 2, depend on the configuration of XML too much; 3, because all messages want to undertake standardizing handling, be generated namely and analytic XML file, can bring about function to drop so; 4, frame of implementation of the need in developing a process is specific interface is received and handle afore-mentioned standard informations, invade a gender strong; 5, documentation is not quite diseased, clear; The advantage of Mule: 1, the framework is simple clarity, easy begin; 2, it has very extensive transmission implement, road by implement with converter, and expand easily; 3, mule does not need to change the word into unified format, and have transition when need only, improved performance; 4, mule code need not pay close attention to in developing a process, need to pass configuration to be able to expose the service only, decreased to invade a gender; 5, documentation is clear and perfect; The defect of Mule: 1, did not implement any ESB standards but abided by " Enterprise Intergration Patterns " with SEDA?

Servicemix的优点： 1，基于JBI规范； 2，可以热部署； 3，支持Camel（可以用DSL去开发集成流程）； Servicemix的缺点： 1，JBI规范带来了使用上的繁琐，且JBI规范没有得到太多的青睐，前途未卜； 2，过多依赖XML的配置； 3，由于所有消息要进行标准化处理，即生成和解析XML文件，所以会导致性能下降； 4，开发过程中需要实现框架特定接口接收和处理上述标准消息，侵入性强； 5，文档不健全、不够清晰； Mule的优点： 1，架构简单清晰、容易上手； 2，它有非常广泛的传输器、路由器和转换器，且易于扩展； 3，Mule不需将消息转换成统一的格式，而只在需要时进行转换，提高了性能； 4，开发过程中无需关注Mule代码，只需通过配置即可将服务暴露，减少了侵入性； 5，文档清晰而完善； Mule的缺点： 1，没有实现任何ESB规范（但遵循了《Enterprise Intergration Patterns》与 SEDA？

Asked for help a 3 D with stronger PSP function, the machine body of this time's setting up a mold would be very outstanding, currently 《the 皇 card air battle X 》of show up a machine body an amount to still can not know so far, but DSuperTomcats F-14, TornadoF3,, F2-a and Foxhounds MIG-31s 4 will be definitely to show up collectively.

借助了PSP较强的3D机能，本次的机体建模将会十分出色，目前为止《皇牌空战X》的登场机体数量目前还不得而知，但F-14DSuperTomcat，TornadoF3，，F2-a以及MIG-31Foxhound四架集体将会铁定登场。

And her daughter can get the piano keys to chat, and then go left, jump armor available, if we do not jump to the left take the hit BOSS, the focus of this strange change in his will, with the corresponding color of magic to hit it, hit Duct End later, at the same time the left side of the castle church door opened, and then move into the right part of the road, the first door below, and then come to the bottom, jump up the right side of the first scene, the far right can be破墙320 ore, and then be able to return to the road and walk up to the Secretary for Education and experience, and his words can have only the sewer, while chest is 250 ore, and finally take the right side of the door, where there are leave, the old lady to take the jack-o'-lantern Baozhu, we can see the true path, to shield jump available, the most right is the BOSS, is a wolf, the attention of multi-running, over BOSS, be cross badge, now go to the castle Chapel, and

和女儿聊天可以得到钢琴键，然后往左走，跳下去可得盔甲，如果不跳下去继续往左走就打BOSS，此怪重点在他会变色，用相应颜色的魔法才能打到它，打完以后得到风管，同时城堡礼拜堂左边的门打开了，继续前进，进入右边岔路部分后，先到下面的门，再走到最下面，右边第一场景往上跳，最右边破墙可以得到320个矿石，然后就可以回到岔路了，往上走可以遇到司教，和他说话可以得只有下水道，途中宝箱是250个矿石，最后走右边的门，这里的路有假，带上老太太给的鬼火宝珠，可以看到真实道路，往跳下可得盾牌，最右边就是BOSS，是一只狼，注意多跑动，打完BOSS，得到十字徽章，现在去城堡礼拜堂了

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失