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On global optimal algorithms, a heuristic 0-1 Integer Liner Programming algorithm is achieved, which combines heuristic enumeration method and revised simplex method. Simulation experiments show that the degree and optimization efficiency in our algorithm is obviously improved compared with the MATLAB 7 bintprog function that is a 0-1 integer programming algorithm. Moreover our algorithm isn\'t relevant to the scale changes of Web services composition and candidate service sets, thus it is applicable for the scenes that the scales of Web services composition changed dynamically. But for Web services composition with service alliance, the algorithm is unable because the non-linear global optimal model of the fitness function and QoS constraints can\'t be expressed in linear mode.

在全局优化算法研究上，基于启发式0-1整线性规划算法结合了修正单纯形法和启发式枚举法，仿真实验表明该算法与MATLAB 7的0-1整数规划方法比较，在优化程度及优化效率上得到了显著改进，并且对服务组合和候选服务集合的规模变化没有明显的相关性，因而该算法可用于服务组合规模动态变化的场景，但该算法不能应用于存在服务联盟的非线性全局优化模型。

I suggest that call for of the class adviser, is to be forced but resign from the position but today , cardiodynia arrives at unable breath!

而今天我却提出辞去班主任的要求，是万不得已，心痛到无法呼吸！

At the same time, the education sphere of learning the research which relates to between the middle and elementary schools and the news media are also quite few, the middle and elementary schools pedagogues are unable in the concrete management activity to be under the effective theo...

同时，教育学术领域对中小学校与新闻媒体之间关系的研究也还比较少，中小学校教育者无法在具体的管理活动中得到有效的理论指导。为此，有必要对中小学校与新闻媒体的关系做一番研究，以帮助基层教育管理者智慧、科学地应对媒体，在信息社会中增强竞争力。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。