查询词典 be unable to get by

There is a kind of monkey trap used in Asia, a coconut is hollowed out and attached by a rope to a tree or stake in the ground, at the bottom of the coconut a small slit is made and some sweet food is placed inside, the hole on the bottom of the coconut is just big enough for the monkey to slide in his open hand, but does not allow for a closed fist to pass out, the monkey smells the sweets, reaches in with hand to grasp the food and is then unable to withdraw it, the clenched fist won't pass, through the opening, when the hunters come, the monkey becomes frantic but cannot get away, there is no one keeping that monkey captive, except the force of its own attachment, all that it has to do is to open the hand, but so strong is the force of geed in the mind that is a rare monkey which can let go, it is the desires and clinging in our minds which keep us trapped, all we need to do is to open our hand, let go of our selves, our attachment and be free.

在亚洲某些偏远地区﹐流传著某种传统捉猴子的陷阱﹐他们把椰子挖空﹐然后用绳子绑起在树上或固定在地上﹐椰子上留了一个小洞﹐洞内放了一些美饵﹐洞口大小恰好只能让猴子空著手伸进去而无法握著拳伸出来﹐於是顽猴闻香而来﹐将手伸进去抓拿美食﹐而后理所当然的﹐手便伸不出来﹐紧握的拳头伸不出洞口﹐当猎人到来时﹐羣猴惊慌失措﹐但﹐就是逃不掉﹐并没任何人捉著猴子不放﹐它﹐只是被本身的执著所俘虏﹐它只需将紧握的手拳头松软放开便可伸出来﹐但是﹐就是内心中贪恋的欲念所致﹐鲜有顽猴能放下﹐因内心中的欲望与执著﹐使得我们一直受縳﹐面对这逆境﹐我们唯一要做的﹐只是将双手释然放下﹐放下自我执著的坚持﹐就能消遥自在了。

Ajax was a man of giant stature,daring but slow.when the trojan war was about to break out he led his forces from salamis to join the greek army at aulis.as one of the trustiest champions of the greek cause,he was given thejob of guarding one end of the greek camp near troy.he was noted in the battlefield for his bravery and courage.after achilles'''' death he became one of the two hot contestants for the dead hero''''s shield and armour,the other being odysseus.when the weapons were finally judged to his rival,ajax went mad for grief.unable to get over the hardships,he took his own life.when,in order to seek the advice of,odysseus came to visit the lower world,the shade of ajax frowned uponhim.in the lower world ajax chose to be a lion,guided clearly by the bitter recollection of his former life.

埃杰克斯是位身材魁梧、骁勇善战、但头脑迟钝的人。当特洛伊战争即将爆发时，他率领军队从萨拉米斯到达奥尔墨斯加入了希腊军队。作为一名为希腊事业奋斗的最可信赖的斗士，他被派去保卫靠近特洛伊的希腊军营边界。战场上，他因作战勇猛而出名。阿基里斯死后，他成为两个最有可能获得阿基里斯的盾甲的有力竞争对手之一。另一名对手是奥德修斯。最后，他的对手胜利了，并得到了盾甲。埃杰克斯悲伤至极，无法自拔，最终结束了自己的生命。奥德修斯为了得到盲人底比斯的忠告而来到阴间；埃杰克斯的幽灵冲着他直皱眉头。在阴间，由于受到前世痛苦记忆的折磨，埃杰克斯选择了做一头狮子。

Ajax ajax was a man of giant stature,daring but slow.when the trojan war was about to break out he led his forces from salamis to join the greek army at aulis.as one of the trustiest champions of the greek cause ,he was given thejob of guarding one end of the greek camp near troy.he was noted in the battlefield for his bravery and courage.after achilles'' death he became one of the two hot contestants for the dead hero''s shield and armour ,the other being odysseus.when the weapons were finally judged to his rival,ajax went mad for grief.unable to get over the hardships,he took his own life.when ,in order to seek the advice of,odysseus came to visit the lower world,the shade of ajax frowned uponhim.in the lower world ajax chose to be a lion,guided clearly by the bitter recollection of his former life.

埃杰克斯埃杰克斯是位身材魁梧、骁勇善战、但头脑迟钝的人。当特洛伊战争即将爆发时，他率领军队从萨拉米斯到达奥尔墨斯加入了希腊军队。作为一名为希腊事业奋斗的最可信赖的斗士，他被派去保卫靠近特洛伊的希腊军营边界。战场上，他因作战勇猛而出名。阿基里斯死后，他成为两个最有可能获得阿基里斯的盾甲的有力竞争对手之一。另一名对手是奥德修斯。最后，他的对手胜利了，并得到了盾甲。埃杰克斯悲伤至极，无法自拔，最终结束了自己的生命。奥德修斯为了得到盲人底比斯的忠告而来到阴间；埃杰克斯的幽灵冲着他直皱眉头。在阴间，由于受到前世痛苦记忆的折磨，埃杰克斯选择了做一头狮子。

Ajax was a man of giant stature,daring but slow.when the trojan war was about to break out he led his forces from salamis to join the greek army at aulis.as one of the trustiest champions of the greek cause,he was given thejob of guarding one end of the greek camp near troy.he was noted in the battlefield for his bravery and courage.after achilles'' death he became one of the two hot contestants for the dead hero''s shield and armour,the other being odysseus.when the weapons were finally judged to his rival,ajax went mad for grief.unable to get over the hardships,he took his own life.when,in order to seek the advice of,odysseus came to visit the lower world,the shade of ajax frowned uponhim.in the lower world ajax chose to be a lion,guided clearly by the bitter recollection of his former life.

埃杰克斯是位身材魁梧、骁勇善战、但头脑迟钝旳人。当特洛伊战争即将爆发时，他率领军队从萨拉米斯到达奥尔墨斯加入le希腊军队。作为─名为希腊事业奋斗的最可信赖的斗士，他被派去保卫靠近特洛伊的希腊军营边界。战场上，他因作战勇猛而出名。阿基里斯死后，他成为两个最有可能获得阿基里斯的盾甲的有力竞争对手之一。另一名对手是奥德修斯。最后，他的对手胜利了，并得到了盾甲。埃杰克斯悲伤至极，没有办法自拔，最终结束了自己的生命。奥德修斯为了得到盲人底比斯的忠告而来到阴间；埃杰克斯的幽灵冲着他直皱眉头。在阴间，由于受到前世痛苦记忆的折磨，埃杰克斯选择了做一头狮子。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。