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Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

This thesis applies default theory which is adaptive to change to the domain of requirements engineering, introduces partial requirements specification , which helps to avoid trivial solution and obtain locally effective result whose correctness can not be absolutely guaranteed. It is similar to our method that people deal with incomplete information world by commonsense.

本文将适应变化的缺省理论应用于需求工程领域，引入部分需求规格，有助于避免平凡解，可得出局部有效但不一定必然正确的结论，这和人类用常识处理非全知世界所采用的方法很相近。

The results of the analysis to complete 18S rDNA and partial 28S rDNA D 6-7 domain sequences of 30 collembolan species supported the monophyly of Poduromorpha and Symphypleona, Entomobryoidea is not a monophyly, familia Neelidae should be separated from Symphypleona. The situation about the superfamilia of Tomoceroidea and the familia Neelidae need further studies. The results of the further analysis to complete 18S rDNA and nearlly completely 28S sequences of 4 Protura, 6Collembola, 5 Diplura, 5 Insecta, 10 Crustacean, using 1 Myriapoda and 1 Chelicerata as outgroups indicated that Protura, Collembola and Diplura are monophy, respectively, Protura and Diplura have the nearest realationship, they make sister groups with Collembola and all of them assemble with Insecta to make a monophy cluster of Hexapod; The results support the monophy of Pancrusracea, Crustacean is not a monophy cluster and the realationships between them with Hexapod need further reseachers.

对我国的14科27属30种跳虫的18S rDNA全序列和28S rDNA部分序列(D6-D7)的基因信息数据进行分析，结果支持原目、愈腹目的单系性，长角目不形成单系群，支持短角科从愈腹目中分出，但鳞总科和短角目的分类地位有待于深入分析探讨；进一步比对4种原尾虫、6种跳虫、5种双尾虫、5种昆虫和10种甲壳动物的18S rDNA全序列和28S rDNA全序列，以1种多足动物和1种螯肢动物为外群，进行系统关系分析，结果显示：原尾纲、弹尾纲、双尾纲分别为单系群，原尾纲和双尾纲优先聚为一支，然后和弹尾纲构成姐妹群，以上三类又和昆虫聚为一支，支持六足动物的单系性，支持泛甲壳动物，但甲壳动物并未形成单系，其各类群和六足动物之间的亲缘关系尚须进一步确定。3。

A photonic crystal-air interface or line defect is utilized as a holophote or a partial reflective mirror to design a paralleled plane cavity, a single ring cavity structure and a multi-ring cavity structure. The method has simple design idea, good integration and wide application; by adopting the method, the self aligning photonic crystal lasers with different wave bands can be designed according to the luminescent characteristics of different materials; particularly, the utilization of semiconductor materials to design a light source of optical communication wave bands has more important practical value.

利用光子晶体-空气界面或线缺陷作为全反射或部分反射镜设计平行平面腔、单环形和多环形腔结构；该方法设计思路简单，集成性好，适用范围广，可根据不同材料的发光特性设计不同波段的自准直光子晶体激光器，特别是用半导体材料设计光通讯波段的光源器件具有更重要的实用价值。

Our studies indicate that the increased contents of glucose and accumulation of active metabolic intermediates due to metaboic disorder contribute to the cutaneous environmental alterations associated with diabetes mellitus.Thus,the cutaneous environmental alteration is one of the initial events for impaired healing.The succession of progressive injury in deep partial thickness burn wounds is carried out depending on the physiological outcome of parabiotic tissues.The loss of dernal template due to the damage to the integrity and the continuity of dermal tissue may be one of the leading reasons of hyperplastic scar formation.

通过对糖尿病合并创面难愈、烧伤创面的进行性损害以及增生性瘢痕形成的机制研究，我们发现：糖尿病代谢紊乱所致皮肤组织中糖含量增高和代谢产物蓄积引起的皮肤微环境改变，是导致糖尿病创面难愈的始动因素之一；烧伤后的组织进行性损害的发生发展取决于&间生态&组织的病理转归；真皮组织缺损导致的模板作用缺失可能是引起增生性瘢痕形成的始动环节。

This project is to make aeroconcrete through the high temp and high pressure to make it into poriferous AAC with the tail quartz sand as the main material, the cement and lime as agglutinant featuring wind block, warm-keeping, sound/heat insulation, water/humidity resistance, antifreezing, vibration proof, acid/alkaline resistance, partial load bearing, large size and weight light, shape formed optionally, moreover, it is a new building material of mast processing possibility since it can be sawed, drilled and nailed so as to save masonry sand and slurry and quicken the process and simplify the operation.

本项目加气混凝土主要是以石英尾砂为基础，以水泥和石灰为胶和剂，经高温高压养护而成的多孔状AAC。

The reporter is in new China discover in manual of raise capital by floating shares, at present business development faces this company a few risks, because collect endowment investment is new door inn amount is more, the likelihood because partial door inn is short-term cannot outstanding achievement of whole of company of gain be a burden on; In addition return inn of existence talent shortage, door and content to flow deserve to send central optional location, management place to rent expire cannot relet or the risk that increase hire.

记者在新华都招股说明书中发现，该公司现在业务发展面临一些风险，由于募资投资新门店数量较多，可能由于部分门店短期不能盈利拖累公司整体业绩；此外还存在人才短缺、门店与物流配送中心选址、经营场所租赁期满不能续租或提高租金的风险。

"Into the world " also bring risk and competition, terraqueous carrying trade is faced with the risk that road carrying trade is facing partial market to lose, part to be fallen into disuse to give the grim situation of the bureau and senior talented person the prediction of a person's luck in a given year of different level.

&入世&亦带来风险和竞争，道路运输业面临着部分市场丢失的风险、部分水陆运输业面临着被淘汰出局的严重形势以及高级人才不同程度的流失等。

And their fractal figures have been drawn. Meanwhile, to obtain a better visual effect and simulate actual natural scene, software adopts the real color and color palette to enrich figures, and color animated cartoon to change them. To show the self-similarity and infinitive tractility of fractal figures, partial zoom has been made on them. In addition, the software also provides several groups of graphic parameters. Very different fractal figure can be created through slightly changing such parameters.

本文运用分形理论实现多种分形算法，在计算机上生成Mandelbrot集，Julia集，L系统，IFS迭代函数系统等典型的分形图形，同时运用真彩色及调色板技术丰富图形的色彩，实现了色彩动画，使其更真实的模拟自然景物；运用鼠标编程技术实现对图形局部的放大和缩小，体现分形图形的自相似性和无限延展性；提供多组参数，利用分形图形的混沌特性，通过微小的参数变化，生成完全不同的分形图形。

Benefit: Devoted to the destruction of the undead, you gain 2 additional turning and remove disease attempts per rest,+1 to your level when turning, a +2 bonus on saving throws against spells and effects produced by undead, and all attacks you make have a chance of destroying undead instantly on what would be a Vorpal strike. Natural 20 followed by confirmation, Will DC: 30 partial, deals Light damage to targets immune to instant destruction effects* or those that save.

能力：倾尽全力于毁灭亡灵，你获得额外2次驱散和移除疾病，驱散时获得额外+1驱散等级，在对抗不死生物的法术和特殊效果时获得+2豁免加值，你的所有攻击都有机会瞬间摧毁命中的不死生物，（自然投出20点并确认重击，过意志DC30，对免疫即死*和通过豁免的敌人造成光属性伤害。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。