查询词典 be of no effect

Objective To explore the effect of sildenafil on NO and NO synthetase after physical exercise at high altitude.

目的 探讨西地那非对高原人体运动一氧化氮及其合酶的影响。

In this exper iment,we took the advantage of RNAi technique,microinjected tinman and singless dsRNA into the early embryos in Drosophila respectively and got these two genes' RNAi phenotypes,which were very similar to that of their mutant,showing heart tube defects or no heart precursors formation.tinman dsRNA eve caused visceral mesoderm defects and the somatic muscles disruption,yet wingless dsRNA only affected heart precursors and had no effect on visceral mesoderm and somatic muscles,indicationg that the heart-related genes dsRNA interference worked effectively and exclusively in Drosophila.

实验运用RNAi技术，分别将tinman和wingless的dsRNA注入果蝇的早期胚胎，得到了这两个基因的dsRNA干扰表型，与两个基因的突变体表型非常相似，都表现为果蝇心脏前体细胞不能形成或心脏管缺失。尤其是tinman基因的dsRNA，还引起了肠中胚层缺失和体壁肌肉组织的紊乱，而wingless基因的dsRNA却只影响心脏的形成，而不影响肠中胚层，说明dsRNA干扰具有非常强的特异性，因而不失为研究果蝇心脏发育基因功能的有效方法。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

First the passivity of motor rotor flux subsystem is proved. Workless forces have no influence on the energy balance equation and have no effect on the asymptotic stability. Then it is discussed that the system can stably track the reference rotor flux without rotor flux observer. At last an adaptive PBC strategy is proposed with regard to the rotor resistance change. A clear and simple design for the speed control subsystem with inner current loops is presented.

证明了感应电机转子磁链子系统的无源性，找到系统能量耗散特性方程中的无功力，它不会影响系统的稳定性，得出无需转子磁链观测反馈即能稳定跟踪转子磁链的参考值，同时考虑电机转子电阻在运行中发生未知变化，设计自适应调节器使系统对转子电阻呈现鲁棒性，构建了带电流内环速度控制系统。

The supplementation of Zn from both ZnO and basic zinc chloride had no effect no fecal score when compared to that of the control group.

与对照组相比，从氧化锌中添加锌和从碱式氯化锌中添加锌对粪便评价没有明显效果

The expression of Bcl-2 was weak or negative; there is no difference among various groups.Research Conclusion: lCadmium can accelerate the apoptosis of GCs and is related with dose; 2Cadmium accelerate atresic of follicular; and increase the number of interstitial gland;3Cadmium enhance the expression of bax, and no affect obviously on bcl-2;4 Selenium restrained the effect of cadmium-induced apoptosis on granulosa cells, but it cant be interdicted completely.

实验结论：1 镉能促进小鼠卵巢颗粒细胞凋亡，并具有剂量效应关系。2 镉能促进卵泡闭锁，使卵巢间质腺数量增加。3 镉能增强颗粒细胞凋亡相关基因bax的表达，对bcl-2的表达则无明显影响。4 硒具有一定的抑制镉致颗粒细胞凋亡的作用，但不能完全阻断。

SIA degree in phacoemulsification with 3.2mm clear corneal incision has no connection with incision positions. Corneal curvature at incision meridian will be released, but another meridian has no distinct effect. The SIA analyzed by Jaffe/Clayman vector method will provide an important reference for toric IOL implantation surgery.

3.2mm透明角膜切口SIA的度数与切口位置无明显关系，切口对所在子午线角膜曲率有松解作用，对与切口垂直子午线角膜曲率无明显影响，矢量公式法计算所得的SIA值在散光型人工晶状体植入术中提供了重要的参考价值。

Study the activity of inducible nitric oxide synthase in the liver of rabbits infected with Eimeria stiedai, the concentration of nitric oxide in the serum、the coccidian oosysts per gram feces ,Score the pathologies according to anatomic structures of rabiits'livers and micoscopic pathological changes. So as to study the effect of exogenous NO and NOS of the rabbits infected with E.stiedai. The results showed that when the rabbits were infected with E.stiedai, the activity of iNOS in the liver and the concentration of NO in the serum gradually increased and they both reached the peak after 12 days in the control group , GTN and GSNO groups.

为了探讨外源性一氧化氮和一氧化氮合酶在家兔球虫感染时的作用，本试验以腹腔注射的途径给予感染斯氏艾美耳球虫的家兔NO供体硝酸甘油和亚硝基谷胱甘肽，对球虫感染家兔诱导型一氧化氮合酶活性、血清中NO的浓度、每克粪便的球虫卵囊数、肝的剖检和镜下病变及其病变计分进行了研究。

The operation is a direct approach road, and very good to dacryocystorhinostomy positioning, no tube, no facial scars and long-term effect is inspirer .

该手术具有进路直接，泪囊定位准确，无须常规置管，面部无瘢痕，远期效果好等优点。

Inside most present bathrooms, an exhaust fan is always used to draw out the wet air but with no drying effect; a heater or heating fan is used for keeping body warm in cold days but with no dehumidifying function; an electric heater is added in mirror to defog but with shortage of wasting energy and taking risk of shock; towels are used to dry the body but likely affecting germ.

在目前的浴室中，大多使用抽气风扇抽走湿气但无乾燥效果；使用加热器或热风扇来作冷天的身体保温但无除湿功能；在镜子以附加电热器作除雾，但有耗能和可能触电的缺点；使用毛巾来擦乾身体但容易传染细菌。

The witness also told the jury at the Royal Courts of Justice in London that he saw a paparazzo fighting with a member of the public who was trying to stop him taking pictures in the minutes before the emergency services arrived.

他还告诉在伦敦皇家法庭的陪审团，在急救服务到来之前，他当时看见一个狗仔队正和一群阻止他拍照的人打架。

The entire N／C program on a tape is made up of an accumulation of these successive data blocks.

纸带上的整个数控程序由这些连续数据单元连接而成。