查询词典 be of no effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The content of each part follow as: In the first chapter, as start point and base of the paper, this part focuses on the basic study ofdefinition characteristic of NO, and the existing base of NO-E-Commerceenvironment; In the second one, this part studies the theory base of NO comprehensively applying the theories of core competence competent strategy and transaction cost; Chapter three studies the NO from the coordination of NO, and gives the structure clarification and characteristic of NO firstly, at the same time, put forward the concept of Virtual Enterprise Cluster; Based on such conclusion, studies the model of NO from life cyc organization level process and value chain, and operational mode; In Chapter four, a theoretical explanation was addressed on the above structure by modeling NO with game theory and graphic theory; In the fifth chapter, on the bases of analysis of NO operational risks, coordination mechanism of NO was studied by individually modeling the NO without core and NO with core, and then put forward the solution for coordination mechanism of NO; As an important component of coordination mechanism of NO, Chapter six explored some basic concept of trust and importantly put forward the way of how to build trust in NO, especially investigated the supporting function of valid reputation mechanism of NO for the trust building, importantly an operational method on building reputation mechanism and evaluation method in NO were given; The last chapter applied theconclusion of the paper to investigate the famous trade Web-SUNBU.

全文共分为七章，主要内容如下：第一章作为全文的理论出发点和基础，围绕网络组织的定义、特征以及网络组织生存基础--电子商务环境等方面对网络组织的基本概念进行了阐述；第二章综合运用核心能力、竞争战略和交易费用理论对网络组织产生的理论基础进行阐述；第三章首先从组织协调的角度对网络组织进行了研究，给出了网络组织的结构，分类和特征，同时并给出了虚拟企业群簇；然后在此基础上分别研究了网络组织的生命周期模型、层次模型、过程模型、价值链模型，以及运行模式；第四章综合运用博弈论、图论的相关知识，通过构建网络组织的模型，对上一章所研究的网络组织结构的形成机理给出了一种理论解释；第五章在分析网络组织运行风险的基础上，分别建立无盟主网络组织的博弈论模型和有盟主网络组织的博弈论模型，详细研究了网络组织的协调机制，然后给出了网络组织协调机制的解决方案；第六章作为网络组织协调机制的重要组成部分，本章在讨论了网络组织中建立信任机制的必要性的基础上，研究了网络组织信任关系的类型，提出了在网络组织中如何建立信任机制。

Two merging methods of Bézier curves are generalized to tensor product Bé zier surfaces, we propose two methods to approximate two pieces of adjacent tensor product Bézier surfaces by a piece of tensor product Bézier surface. We offer the explicit formulas of the merged tensor product Bézier surfaces with all kinds of merging conditions.

同样，Bézier曲线的两种合并方法也被成功的推广到张量积Bézier曲面上，得到把两片相邻张量积Bézier曲面合并成一片张量积Bézier曲面的两种合并方法，并给出在各种合并条件下的合并曲面控制顶点的显示表示式。

The research indicated that,the 36°Bè xylose mother liquor was used as material,9.60% catalyst M-16 was added,and after caramelized 180min at 120℃,a caramel...

研究表明，在36°Be′的木糖母液中，加入9.60%的M-16催化剂，于120℃下焦糖化反应180min，得到色率为2.8万EBC单位，红色指数达6.3以上的焦糖色素。

I can ride my bike with no handlebars No handlebars No handlebars I can ride my bike with no handlebars No handlebars No handlebars Look at me, look at me hands in the air like it's good to be ALIVE and I'm a famous rapper even when the paths're all crookedy I can show you how to do-si-do I can show you how to scratch a record I can take apart the remote control And I can almost put it back together I can tie a knot in a cherry stem I can tell you about Leif Ericson I know all the words to "De Colores" And "I'm Proud to be an American" Me and my friend saw a platypus Me and my friend made a comic book And guess how long it took I can do anything that I want cuz, look: I can keep rhythm with no metronome No metronome No metronome And I can see your face on the telephone On the telephone On the telephone Look at me Look at me Just called to say that it's good to be ALIVE In such a small world I'm all curled up with a book to read I can make money open up a thrift store I can make a living off a magazine I can design an engine sixty four Miles to a gallon of gasoline I can make new antibiotics I can make computers survive aquatic conditions I know how to run a business I can make you wanna buy a product Movers shakers and producers Me and my friends understand the future I see the strings that control the systems I can do anything with no assistance Cuz I can lead a nation with a microphone With a microphone With a microphone And I can split the atom of a molecule Of a molecule Of a molecule Look at me Look at me Driving and I won't stop And it feels so good to be Alive and on top My reach is global My tower secure My cause is noble My power is pure I can hand out a million vaccinations Or let'em all die in exasperation Have'em all healed of their lacerations Have'em all killed by assassination I can make anybody go to prison Just because I don't like'em and I can do anything with no permission I have it all under my command Because I can guide a missile by satellite By satellite By satellite And I can hit a target through a telescope Through a telescope Through a telescope And I can end the planet in a holocaust In a holocaust In a holocaust In a holocaust In a holocaust In a holocaust I can ride my bike with no handlebars No handlebars No handlebars I can ride my bike with no handlebars No handlebars

我可以坐我的自行车没有把手没有把手没有把手我可以坐我的自行车没有把手没有把手没有把手看我，看我手在空气中一样，它的良好的可活着和我是一个著名的说唱甚至当paths're所有crookedy 我可以告诉您如何做硅做我可以告诉您如何从头纪录我可以采取除了远程控制我几乎可以把它连同我可以配合一结在一个樱桃干我可以告诉你，约莱夫帕格ericson 我知道的所有字眼，以"德colores "和"我很自豪地成为美国"我和我的朋友看到一个鸭嘴兽我和我的朋友作出了漫画书和猜测多久才我可以做任何事情，我想cuz ，请看：我可以保持节奏，没有节拍器没有节拍器没有节拍器我可以看到你的脸就电话关于电话关于电话看我看我只是所谓的说，它的良好的可活着在这样的一个小世界我所有的卷曲与一本书，阅读我可以赚钱，打开一个勤俭建国的商店我可以生活小康杂志我可以设计一个发动机64 英里一加仑汽油我可以做出新的抗生素我可以使电脑的生存，水生条件我知道如何执行业务我可以让你想购买的产品提出动议的热点和生产者我和我的朋友们了解未来我看到的字符串控制系统我可以做任何事情，没有援助 cuz我可以导致一个民族，一个麦克风与麦克风与麦克风我可以分裂了原子的分子一个分子一个分子看我看我驾驶，我不会停止和感觉那么好要活着就顶端我的达成是全球我的大楼安全我的事业是高尚的我的权力是纯我可以伸手万疫苗接种或let'em都死在无奈 have'em全部愈合他们的割伤 have'em所有杀害暗杀我可以作出任何人去监狱只是因为我不和like'em 我可以做任何事情，没有许可我一切都在我的指挥因为我可以引导导弹卫星通过卫星通过卫星我可以击中目标，通过望远镜透过望远镜透过望远镜我可以结束的星球在大屠杀在大屠杀在大屠杀在大屠杀在大屠杀在大屠杀我可以坐我的自行车没有把手没有把手没有把手我可以坐我的自行车没有把手没有把手

The effect of treatment is negatively correlated with the course of disease, generally, the effect is much worse with the course of disease is much longer; The effect is positively correlated with the course of treatment, it is better if the course of treatment is longer.The group of more than one year is much excellent than the group of less one year. The ex-electroencephalogram before treatment is normal, on edge and a little abnormal, the effect is better. As regards CT or MR result, there is difference between the effective group and the ineffective group. The result of CT and MR is negative, the effect is better, and the effect to demyelination and leukoencephalopathy is most worst.However,for the tipe of much abnormality, the effect is the most worst.The effect is related to course of disease.The effect is better if the course is shorter.There is no difference in the tipe of disease the symptoms before episode, the frequency of episode, the inducing reasons, the age, sex and so on.

病程的长短与疗效存在负相关关系，病程越长疗效越差；疗程与疗效存在正相关关系，疗程越长疗效越好；疗前脑电图的异常程度与疗效存在负相关关系，正常、边缘及轻度异常者，疗效较好，重度异常者疗效最差；无效组与有效组在CT及MR检查结果上有差异，有阴性者疗效好，阳性者疗效差，脱髓鞘及脑白质病疗效最差的趋势；疗效与性别无相关性；无效组与有效组在发作类型、既往病史、辨证分型、发作先兆、疗前发作频率、诱因、发病年龄、就诊前所用药物方面无差异。

No cracks - No corrosion - No rust - No dents - No material defects - No lacquer defects - No surface defects - No deformation - No dirt and dust pins - No malfunction - No sharp edges - No dangerous materials - No colour differences or defects - No wobbling - Bucklings, dents, bumps and runny perpendiculars are not allowed - All materials, especially those which are exposed to mechanical load, have to be free of tension cracks - No surface defects, bubbles, cracks, scratches, deformation.

无裂缝，无腐蚀无锈无金属桶无材料缺陷无缺陷漆器-无表面缺陷，不变形无污垢和灰尘pins无故障没有锋利-任何危险材料，没有肤色的差异或缺陷，不抖动- bucklings ，金属桶，卜薪和流淌perpendiculars不允许所有材料，尤其是那些暴露于机械负荷，房维生素E可以自由张力裂缝，无表面缺陷，气泡，裂纹，划痕，变形。

As a whole, this article investigates the investment effect problem from a new view of regional economics. It covers the economic, social and ecological effect of regional investment; it defines the concepts of investment effect and regional investment effect, deciphers the connotation, classification and influential factors of investment effect, and establishes a basic theory framework of investment effect research; constructs a fire-new regional investment effect evaluation model that could evaluate comprehensively the economic, social and ecological effect of regional investment, including evaluation index system and evaluation method, which supplies the gap of regional investment effect evaluation in academic community.

总体上看，本文从区域经济学这一新的视角研究投资效应问题，内容涵盖了区域投资的经济效应、社会效应和生态环境效应；界定了投资效应、区域投资效应的概念，解读投资效应的内涵、分类与影响因素，建立了投资效应研究的基本理论框架；构建一个对区域投资的经济效应、社会效应和生态环境效应进行综合评价，包括评价指标体系和评价方法在内的全新的区域投资效应评价模型，弥补了学术界在区域投资效应评价方面的空白。

I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Said you're going with your boys today and "Don't worry cause I'll be home late" But I know you're creepin' out there, cheatin', You've been freakin' babe So let me tell you how it's going to be And you can run game but not on me boy So who the hell are you trying to kid You know this is what I did I called your pager and your two-way boy but no response Start looking and I found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Baby boy I know you'll never find No stuff as good as mine She can't work you like I did You can look but you know I got that good shit I know that you're hating it But you better stay with the one you're with You made your bed and now you have to sleep in it baby I called your pager and your two-way boy but no response Start looking and found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door I ain't gonna take this no more I ain't gonna take this no more Bounce baby out the door Bounce baby out the door Get up and move Get up and move Don't make me act a fool Don't make me act a fool Just bounce baby out the door Wait a minute Sarah, give me a chance to explain I was just talking to shorty, I don't even know her name I ain't looking for no one to come and take your place Ain't no one out there baby that can fill your space No need to find a nickel got a dime at home Plus I love every night the freaky way we bone I ain't trying to get gone, ain't trying to roll I'd rather stay and be a part of your green eyed soul Just bounce baby out the door Bounce baby out the door Just bounce I ain't gonna take this no more Just bounce Bounce baby out the door Just bounce Get up and move Just bounce Don't make me act a fool Just bounce

我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待出去门弹起当作婴儿般对待出去门我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑假定你是去用你的男孩今天和"做不担心原因我希望在家迟"但是我知道你是creepin'出去那里cheatin'你已经是freakin 婴儿那样让我说出你，它怎样正将是和你能变得勇敢了但是不是在我上那样地狱对是你试一试的男孩哄骗你的知道这个是我所做的我呼吁你的寻呼和你的双向的男孩但是没有看和我反应出发发现你在情妇手臂不努力尝试的一些中我有我的自己眼睛的仅仅把厌烦你的我被的材料男孩原因带到那里听你所有的谎言处理前面的男孩原因你我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑小男孩我知道你希望从不裁决不材料实际已经开采矿她不能工作你相似我是做你罐看但是你知道我得到那个好排粪我知道那个你是讨厌它但是你更好些留下用一你是用你制做你的床和现在你富人向睡觉在中它当作婴儿般对待我打电话你的寻呼和你的双向地男孩但是没有反应开始看和发现你在中一些情妇's手臂做不请尝试向前男孩原因我拉来拉去你那里用我自己的眼睛仅仅到达你的材料男孩原因我被使厌烦的听见你所有的谎言弹起当作婴儿般对待从里面门我 ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门弹起当作婴儿般对待从里面门到达沿着和移动到达沿着和移动做不制造我行动a开玩笑做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起等候a分钟萨拉让步我a机会向说明我是仅仅说话向矮子我做不平稳知道她名字我ain't寻找没有任何人向来和发生作用你的地方Ain't没有任何人从里面那里当作婴儿般对待那个罐充满你的是一部分你的绿色是从从门公正弹起里面门公正弹起弹起小里面盯着精神公正弹起小我ain't去把这没有更公正弹起弹起小带到从门里面仅仅弹起组织和移动公正弹起不使我装出一傻瓜公正弹起的样子

This does not cause any tension, as if the sea is the cradle of his childhood, and you are the children that sleep, no anxiety, no pain, no irritability, no bustling, no stuporous, no impulse, no temptation, no piles of work, no intrigues cling, there is no noise, no confusion, no frustrations, no flickering figures, there is no need to comply with the schedule.

这丝毫不会引起任何紧张，仿佛大海就是童年的摇篮，而你就是那个酣睡的孩子，没有焦虑，没有痛苦，没有烦躁，没有熙熙攘攘，没有纸醉金迷，没有冲动，没有诱惑，没有堆积如山的工作，没有勾心斗角的攀附，没有吵闹，没有迷惑，没有困顿，没有闪烁的数字，没有必须遵守的日程。

Salt is good, but if the salt becomes flat and tasteless, with what do you season it?

14：33 盐本是好的，盐若失了味，可用什么叫它再咸呢？

He reiterated that the PLA is an army of the people under the leadership of the Communist Party of China.

他重申，人民解放军是在中国共产党领导下的人民军队。