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In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

To help improve Henan Netcom Company's ability of competition, we also make the environmental analysis, the strategy analysis and the competitive analysis; distinct it's external internal strength as well as weakness opportunity, threat. We assure that the core competition abilities include advanced telecommunication network, large numbers of subscribers and various services. Then this text analysis the competitors, the potential competitors, substitutions, equipment providers and customers by using Porter theory, compare each competitor's ability and the field that they compete drastically. Through conforming the costumer's utilitarian and personal services requirement, it discover that the key factor of development is holding the group subscribers. By Boston Matrix we find out key services, staring services, problem services, then study which service is the most important for us and which one should be throw off. Base on analysis above, this article concludes the general strategy and the service strategy of Henan Netcom: enforce the stable development strategy for certain period, and perform expansion strategy when time is fit.

在对河南省通信公司的发展战略的分析中，以战略管理理论战略分析为基础，进行了河南省通信公司的内部的优势和劣势以及外部机遇和威胁分析，确定了河南网通公司的优势是先进的通信网络、庞大的客户资源和丰富的业务；运用波特五种竞争力分析方法，通过竞争对手分析、潜在竞争对手分析、替代品分析、供应商分析、用户分析，探讨了几个竞争者实力对比和相互竞争激烈的领域；明确了用户需求是在方便和实用的同时，追求个性化服务，得出了今后竞争的重点是保住关键的大客户；运用波士顿矩阵分析法，找出了河南省通信公司的金牛业务、明星业务和问题业务，研究了公司各种业务产品的发展重点和取舍战略。

In accordance with the internationalization of professional service standards, as well as at the effective implementation of domestic experience, we can provide customers with a single contact, the internal services and external service take into account the complete service delivery model to help clients reach business decision-making, and limited resources will be the most rational and effective utilization.

按照国际化的的专业服务标准，以及在国内有效实施的经验，我们能为客户提供单一联络、内部服务和外部服务兼顾的完整服务模式，协助客户达成业务决策，并使有限资源得到最合理有效的利用。

Heard of other people s children can sleep the whole three months of sleep, and some milk at night to eat can be 1,2 times, and I only eat the 宝宝 two or three times before the past two weeks, every night is necessary to every one and a half hours to eat one, exclusive breastfeeding, and do not feed him he s not hungry, eat the hands, and then began to cry not, in any case do not feed him he will not allow, much less sleep I tried to pacify him,喂水, or not, one night I have to do to feed his 6,7 times, urine is also very much about my dire strait, and during the day to take care of him, basically what睡不成, I will go on like this collapse, we quickly think of a way to help me, my first time when the mother, lack of experience, I hope you give me the ideas!!!

三个多月宝宝晚上总要奶吃怎么办？急！！！听说别人的宝宝三个月就可以睡整觉了，有的晚上吃1、2次奶就可以了，我的宝宝以前还只吃两三次，最近两个星期，每天晚上每隔一个半小时就要吃一次，纯母乳喂养的，不喂他他就饿的不行，就吃手，再不行就开始哭闹，反正不喂他他就不让，更不睡，我试过安抚他，喂水、还是不行，一宿我要喂他6、7次才行，尿的也非常多，我快要困死了，白天还要照顾他，基本上睡不成什么，再这样下去我会崩溃的，大家快帮我想想办法吧，我第一次当妈妈，没有经验，希望大家多给我出出主意！！！

Wheaton is part of a unique experiment that will help determine whether voluntary measures can be as effective as quotas in feminising the white, middle-aged male profile of top boards .

惠顿参与了一项独特的试验，帮助确定自愿举措是否与配额制同样有效。这里的配额制，就是在充满中年白人男性的董事会中，规定女性董事必须达到一定比例。

Can modify whole station program namely at most, 2007, I did my individual website, content of it doesn't matter, the page is simple, do not have discharge at all, because do not have time, also not was in charge of a website all the time, 08 year arrived, I have enough time bubble to be on the net, begin to care the station that has me to come, made the best of time to arrange the website one time, made some of self-help chain, sent dot rubbish to stick promotion to other forum, ah, do not see effect, wander in a few Ip everyday, really pained, the night of Yuanxiao, a person is dull, net of all night on preparation, admire to comparative on the net according to the door in the discovery on the net innocently fire, I had not heard of this thing before, do not follow to go up tide, do not have method ~~, one is searched on the net, stick in Baidu good found a few pieces of relevant pictures not easily, ah, stick to my forum immediately.

顶多就是能修改一下整站程序，2007年，我做了我的个人网站，没什么内容，页面简单，根本没流量，由于没时间，也就一直没管网站，08 年到了，我有充足的时间泡在网上，开始关心起我的站来，抓紧时间把网站整理了一番，做了些自助链，到其他论坛发了点垃圾贴推广，呵呵，不见成效，天天徘徊在几十个ip，真是苦恼，元宵之夜，一个人无聊，预备上通宵网，无意在网上发现&艳照门&在网上相当火，之前我还没听说这事，跟不上潮流，没办法~~，在网上搜索了一把，在百度贴吧好不轻易找到了几张相关图片，呵呵，马上贴到我的论坛上。

The place for the item in HongSe Village, HongKou, Town, Du Jiangyan City lie on the height of 1100 meters. It is fit to plant the two kinds of kiwis of Red Sun and Sea Water, especially the Red Sun, it has a kind of unique flavor, and it can help to be more beauty, avoid the cancer, and digest.

都江堰虹口乡红色村，项目用地位于海拔1100米的地方，非常适应种植&红阳&和&海沃特&两种猕猴桃，特别是&红阳&，它有独特的风味，吃后能美容，抗癌，帮助消化的功效。

Motherfukkers wanna act loco, hit em wit, numerous shots with the fo'-fo' Faggots runnin to the Po-Po's, smoke em like cocoa Fuck rap, coke by the boatload Fuck dat, on the run-by, gun high, one eye closed Left holes through some guy clothes Stop your bullshittin, glock with the full clip Motherfukkers better duck when the fool spit One shot could make a ***** do a full flip See the ***** layin shocked when the bullet hit I hate my high youth, no niggaz wanna buy you But see me I wanna fuck for free Now I gotta let her take this ride, make you feel it inside your belly, if it's tight get the K-Y Jelly All night get you wide up inside the telly Side to side, til you say Jay-Z you're too much for me I am a little bit of loneliness A little bit of disregard Handful of complaints But I can't help the fact That everyone can see these scars I am what I want you to want, what I want you to feel But it's like no matter what I do I can't convince you to just believe this is real So I let go watching you Turn your back like you always do Face away and pretend that I'm not But I'll be here 'cause you're all that I got Got a condo with nothin but condoms in it The same place where the rhymes is invented So all I do is rap and sex, imagine how I stroke See how I was flowin on my last cassette?

motherfukkers不愿扮演买卖，击中在束手无策，无数镜头与fo'佛' faggots runnin向蒲圃的，烟在想可可他妈的说唱，焦炭，由船他妈的数据，对我们来说处，枪高，睁一眼闭一眼，左孔，通过一些家伙衣服停止你的bullshittin ，格洛克与充分剪辑motherfukkers更好的鸭当傻瓜随地吐痰一杆可以做出*****做了充分倒装看到***** layin震惊时，一颗子弹击中我恨我高青年，没有niggaz想购买，但你看我，我想他妈的免费现在，我的宝贝，让她在此转乘，使你感到这里面你的肚子，如果它的紧迫得到健扬果冻一整夜，你得到了广泛的内telly方方， TIL的，你说的Jay - Z你太多，我我感到一点点的寂寞一点点的漠视少数投诉不过，我可以帮不了事实，即每个人都可以看到这些伤疤我什么我要你想，我想你的感觉，但它的，不管是什么，我我无法说服你只是相信这是真实的，所以我放手看着你谈谈你的背部一样，你总是做脸去，假装我不是但是，我可以在这里'的事业，你都是我的一个共管与nothin但是避孕套在这同一个地方，而语言是发明所以，我要做的就是Rap及性别，想象我如何中风，看看如何，我flowin对我的最后卡式？

I was put into a jail once on this account, for one night: and, as I stood considering the walls of solid stone, two or three feet thick, the door of wood and iron, a foot thick, and the iron grating which strained the light, I could not help being struck with the foolishness of that institution which treated me as if I were mere flesh and blood and bones, to be locked up.

我还一度为此进过监狱，关了一夜。当我站在牢房里，打量着牢固的石壁，那石壁足有二、三尺厚，铁木结构的门有一尺厚，还有那滤光的铁栅欗。我不由地对当局的愚昧颇有感独。他们对待我，就好像我不过是可以禁锢起来的血肉之躯。

I was put into a jail once on this account, for one night: and, as I stood considering the walls of solid stone, two or three feet thick, the door of wood and iron, a foot thick, and the iron grating which strained the light, I could not help being struck with the foolishness of that institution which treated me as if I were mere flesh and blood and bones, to be locked up.

我还一度爲此进过监狱，关了一夜。当我站在牢房里，打量着牢固的石壁，那石壁足有二、三尺厚，铁木结构的门有一尺厚，还有那滤光的铁栅欗。我不由地对当局的愚昧颇有感独。他们对待我，就好像我不过是可以禁锢起来的血肉之躯。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。