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As for the ester exchange reaction on phosphorus, it was found that N-phosphoryl histidine the fastest one among all of the N-phosphoryl aminoacids. The results studied by MNDO method indicate that due to theparticipation of imidazole group, the formation of hexa-coordinatephosphorus intermediate made the iso-propoxyl which is opposite toimidazole group more active than the other ones and much easier to leave.When iso-propoxyl left, a new penta-coordinate phosphorus intermediate wasformed, and it is sterically favorable to be attacked by an alcohol from theopposite direction of imidazole.

对于磷上酯交换反应,实验中发现N-磷酰组氨酸反应速度明显高于其它N-磷酰氨基酸,理论研究表明,侧键咪唑基参与下六配位磷中间体形成后,位于咪唑基对面的异丙氧基反应活性大大提高,即从咪唑基的对面位置发生异丙氧基的离去和另一分子醇的进攻,在能量上和空间上都是有利的。N-磷酰丝氨酸的磷上酯交换反应有着类似的特点。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,自9例慢性HBV感染患者血清中扩增HBV X基因,克隆入pGEM Teasy质粒,随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守,但每个序列均不一致。X区除了存在广泛的碱基点替换突变外,序列的缺失突变占测序克隆总数的51.4%(19/37);氨基酸缺失及移框突变多发生于123位氨基酸残基之后,可导致X蛋白多种羧基端形式。

According to the understanding of this unconfused union, we confess the Blessed Virgin to be Theotokos, because the Word of God was incarnate and made man, and through her conception united to Himself the temple He received from her.

据了解,这unconfused工会,我们承认圣母将子,因为天主的圣言是体现了人,并通过她的观念统一自己的寺庙,他收到了她。

Last year a friend of mine sent me some very special Ume from Osaka and I made my own Umeshu, 5 litres to be precise, it takes about 6 months to become ready to drink.

去年,我的一个朋友送我一些非常特殊的梅从大阪和我做我自己的Umeshu,5升确切地说,它需要大约6个月已准备好饮料。

Last year a friend of mine sent me some very special Ume from Osaka and I made my own Umeshu, 5 litres to be precise, it takes about 6 months to become ready to drink.

去年,我的一个朋友给我一些非常特殊的梅大阪我和我自己的Umeshu,5升确切地说,它需要大约6个月已准备好饮料。

To correctly grasp the immense contribution that the masses have made during the social history s development,obey the mass line,set up the correct mass view,insist on and explore the mass working methods in our Party s new period,realize the mass working system,be on behalf of most masses essential interest permanently,and absorb the unexhaustive strength from the masses to go ahead.

正确认识人民群众在社会历史发展中的巨大作用,走群众路线,树立正确的群众观点,坚持和探索党在新时期的群众工作方法,实现群众工作制度化,始终代表最广大人民群众的根本利益,从人民群众中汲取前进的不竭力

Therefore, it is of great urgence for retail enterprises to make good use of the opportunity that made by electronic business and information technology and to make out appropriate strategy so that the core competitive capacity can be immediately improved to meet the challenge from foreign competitors ,which serves as an exceedingly practical subject for research.

因此对于我国零售企业来说,怎样利用电子商务、信息技术所提供的机遇,制定适当的网络营销策略,通过恰当的方法提升零售企业的竞争力,迎接国外竞争的全面挑战,是一个非常现实的研究课题。

When you come to Pieria, you have got to taste the fresh and tender sea bass from the Aegean Sea, the roasted and succulent mouton wrapped in cabbage leaves, the home-made-like battered fried courgette, and special flavored pickled olives. And for sure, these should be accompanied by the fragrant Tsipouro or fennel taste Ouzo.

来到比爱利亚,一定要尝一尝爱琴海新鲜肥嫩的海鲈鱼、用蔬菜包着烤得汁水淋漓的小羊肉、宛若邻家大婶做的炸西葫芦饼、口味独特的腌橄榄,当然,还要佐以芬芳馥郁的齐普罗酒或茴香口味的乌宙酒。

And shaq'n from the look to the as i say hover man with more heart than hallmark on valentine's day i'm the one that you've been raven about, like ray lewis i think it hard to go and change your route cause you don't know if i'm blitzin or if i'm sittin and readin waitin for you to go and trip, drop back and throw up a brick, man ain't no way they can - blow, ain't no way they can - blow ain't no way they can - blow, ain't no way they can - blow ain't no way they can stop me now nelly cause i'm on my way, i can feel my ring comin it's the blood of a champion, pumpin deep inside my veins, too much pride to be runnin i'ma get what i can and more, even if my blood, my sweat, and my tears don't mean nothin it's the heart of a champion (it's the heart of me)(it's the heart of a..) in me it's like a big time decision made on how i can twurk it if hard work pays off then easy work is worthless my work habit ain't no habit man, i do it on purpose i push myself to the limit so my talent'll surface so now it's, curtains and drapes, on anybody who hates dislikin what i'm recitin, bitin wh

和shaq'n 从外观到,正如我所说悬停男子更多的心比标志,在情人节那天我就是您一直在乌鸦约,像射线刘易斯我觉得很难去改变您的路线原因你不知道如果我blitzin或如果我sittin和readin waitin为您去访问,下拉回,并投掷了一个砖,男子是不是没有出路,他们可以-打击,是不是没有出路,他们可以-打击是不是没有出路,他们可以-打击,是不是没有出路,他们可以-打击是不是没有出路,他们可以阻止我现在娜丽事业,我对我的方式,我可以感受到我的戒指comin 它的血液中的一个冠军, pumpin 我内心深处,我的静脉,太多的骄傲要runnin 我得到什么,我可以和更多的,即使我的血,我的汗水,和我的眼泪,并不意味着nothin 它的心脏,一个冠军在我它就像一个大的时间作出的决定,关于如何,我可以twurk它如果辛勤工作,付出过那么容易的工作是毫无价值我的工作习惯,是不是没有习惯男子,我这样做的目的 i推自己到了极限,所以我talent'll表面所以现在它的,窗帘和窗帘,对任何人谁不喜欢 dislikin什么我recitin , bitin褔利

We believe and confess that Jesus Christ, who is the end of the law, hath made an end, by the shedding of His blood, of all other sheddings of blood which men could or would make as a propitiation or satisfaction for sin; and that He, having abolished circumcision, which was done with blood, hath instituted the sacrament of baptism instead thereof, by which we are received into the Church of God, and separated from all other people and strange religions, that we may wholly belong to Him whose ensign and banner we bear, and which serves as a testimony unto us that He will forever be our gracious God and Father.

我们相信,并坦白地说,耶稣基督的人,谁是最后的法律,祂所作出了年底,由换掉他的血液,其他一切sheddings的血液,官兵们还是会作为一个propitiation或满意度为单,并认为他因废除割礼,这是做血液,祂所提起圣事的洗礼,而不是时,是由我们所收到的成神的教会,并脱离所有其他人,奇怪的宗教,我们能完全属於自己的旗和一面旗帜,我们的承受能力,并提供了一个证词所不欲,我们说,他将永远是我们的慈悲上帝和父亲。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。