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Fifty-three primer combinations were found to amplify nuclear DNA of Chinese cabbage, properly. They amplified the fragments up to 4 381 with an average of the 83. Among them, 9 markers derived from 9 primer combinations were linked to the clubroot resistance gene.

筛选出的适宜53对引物组合共扩增出4381条带，占总扩增片断的33.50%，平均每对扩增出83条，其中9个组合扩增出与抗根肿病基因连锁标记。

The result indicated that 237 combinations amplified a total of 13 079 AFLP fragments with an average of 55 fragments, the average of 1.30 markers per 1 000 AFLP fragments were found to be linked to clubroot resistant gene; the amplified polymorphic fragments were 3 167 between the two parents, account for 24 percent of polymorphic frequency; the number of amplified fragments and the number of polymorphic fragments was in positive linear correlation; GC content of AFLP primer combinations significantly affects the number of amplified fragments and the number of polymorphic fragments, indicating negative linear correlation between them.

结果表明：平均每个组合检测到55条带，共扩增13079条带，平均每1000条带检测到1.30个根肿病抗性基因连锁标记；多态性带数为3167条，双亲间多态性频率约为0.24；扩增带数与多态性带数呈线性正相关，引物组合中的不同GC含量与扩增带数和多态性带数存在显著性差异，并表现出线性负相关。

Mthods: The serum levels of IL-15 were measured by a double-antibody sandwich enzyme-linked immunosorbent assay in 47 severe hepatitis B patients and 20 healthy subjects. Parameters of liver function including alanine aminotransferase and total bilirubin were detected by automatic biochemistry analyzer, meanwhile prothrombin activity were measured by automatic coagulator. In the same time, the hepatitis A, B, C, D and E virus markers were examined in all patients.

方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎患者和20例体检健康者血清IL-15的水平，并通过全自动生化仪检测血清丙氨酸转氨酶、总胆红素，全自动血液凝固仪检测凝血酶原活动度，同时采用ELISA方法检测甲、乙、丙、丁、戊型肝炎病原学。

One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group.Immune indexes were examined by using immune staphytococcal protein A rosette test,indirect enzyme-linked immunosorbent assay,cellular culture technique and MTT method.

将180只14日龄雏鸡随机分为毒害艾美球虫初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。

Identification of the markersin F2 plants of 95-5383×HB1 showed that the codominant marker 〓 isclosely linked to the resistance gene in 95-5383,with genetic distance of 2.1cM.

用该引物分析95-5383×HB1的F2个体，〓与95-5383抗病基因的遗传距离为2.1cM。

By bulked segregant analysisin F2progenies of 95-5383×HB1,we have identified one codominant RAPD marker〓 closely linked to SMV resistance gene.

利用BSA法对95-5383×HB1的F2代进行鉴定，筛选出与SMV抗病基因紧密连锁的共显性RAPD标记〓。

The cause of vaginal cancer is closely linked to cervical cancer, and HPV infection seems to be a necessary cofactor in most cases.

引起阴道癌的原因与子宫颈癌密切相关，HPV感染似乎是多数案例的共同必要因素。

Students in the hearts of closely linked, and this is the cohesion of the class!

62名学生的心紧紧连在一起，这就是班级的凝聚力！

It may be linked to a substance called collagen that helps to hold the bones together in a joint.

它可能与一种物质的所谓胶原蛋白有利于举行的骨头一起在一项联合。

The results show that the synthesized electrophoretic coating has overcome the weaknesses of electrophoretic coatings cured by applied cross-linked agent and has strengths such as colorability, high corrosion resistance and decorative results.

该阴极电泳涂料克服了外加交联剂固化电泳涂料的缺点，具有可着色性、高耐蚀性和装饰性等优点。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。